| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,789 | A→T | 14.6% | F362I (TTC→ATC) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,789 | 0 | A | T | 14.6% | 213.4 / 26.6 | 82 | F362I (TTC→ATC) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (30/40); new base T (8/4); total (38/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCG > minE/311722‑311859 | gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:2214056/71‑1 (MQ=255)gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:1888094/71‑1 (MQ=255)gcAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:869617/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGTCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:75692/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:1157785/71‑1 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt > 1:2433711/1‑71 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt > 1:173217/1‑71 (MQ=255) aaGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCt < 1:96267/71‑1 (MQ=255) ggACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:1101363/1‑70 (MQ=255) gACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTg > 1:3538603/1‑69 (MQ=255) gACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa < 1:980473/71‑1 (MQ=255) aCGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:2153029/1‑71 (MQ=255) cGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAg > 1:1111932/1‑70 (MQ=255) gCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa < 1:2798570/71‑1 (MQ=255) aaGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:92396/1‑68 (MQ=255) aaGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGaa > 1:98439/1‑68 (MQ=255) aGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGtt < 1:1603528/58‑1 (MQ=255) ggTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGAt < 1:2509573/71‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa > 1:1460361/1‑58 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCa > 1:3491954/1‑58 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:1341076/71‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:3224834/71‑1 (MQ=255) ccGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt < 1:2069847/71‑1 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACAt > 1:1429650/1‑70 (MQ=255) cGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATa < 1:3872070/71‑1 (MQ=255) gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCTCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:119129/1‑71 (MQ=255) gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:3168855/1‑71 (MQ=255) gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:1819928/1‑71 (MQ=255) gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:2923309/1‑71 (MQ=255) gAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATaa > 1:3833828/1‑71 (MQ=255) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:571554/3‑71 (MQ=2) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:3808043/3‑71 (MQ=2) aacAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:1167036/3‑71 (MQ=2) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:1292292/1‑47 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGaa > 1:2579188/1‑47 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc < 1:1282483/71‑1 (MQ=255) aGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc > 1:2781428/1‑71 (MQ=255) gCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc > 1:3407060/1‑70 (MQ=255) acAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc < 1:1004605/69‑1 (MQ=2) cAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAAc > 1:542339/1‑69 (MQ=255) cAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCa < 1:1035660/71‑1 (MQ=255) cAACACCACGGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCAc > 1:861068/1‑69 (MQ=2) tACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:2039763/62‑1 (MQ=255) caccacgGGTACCGCAATGGTGGGGATCAGCGTTTCTCGCATATTCTGCAAGAACAGATACATCACCAgg > 1:198794/2‑70 (MQ=9) caccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGa < 1:1335111/70‑1 (MQ=9) caccacgGGTACCGCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGa < 1:1648415/70‑1 (MQ=9) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:2530623/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:1643001/1‑70 (MQ=255) ccaccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAac < 1:2281146/71‑1 (MQ=255) caccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa > 1:2588772/1‑69 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac < 1:1917300/71‑1 (MQ=255) accGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAA‑CAGGAacac > 1:376607/1‑70 (MQ=255) ccGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGa < 1:600954/66‑1 (MQ=255) cGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGt < 1:3039447/38‑1 (MQ=255) cGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAacac > 1:3109271/1‑69 (MQ=255) ccgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcat < 1:3386862/68‑1 (MQ=9) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:3751181/59‑1 (MQ=255) cGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatg < 1:2025039/71‑1 (MQ=255) ggCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatga < 1:361876/71‑1 (MQ=255) cgCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGcataa > 1:363357/2‑69 (MQ=9) gCAATGGTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACAtca > 1:726613/1‑50 (MQ=2) cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc > 1:2326278/1‑71 (MQ=255) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc < 1:1798241/70‑1 (MQ=255) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCg < 1:1150541/71‑1 (MQ=255) aTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACa < 1:2911672/40‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCt > 1:207882/1‑71 (MQ=255) ggTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCtt > 1:3127576/1‑71 (MQ=2) tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACa < 1:148009/42‑1 (MQ=255) tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCg < 1:2622455/71‑1 (MQ=255) aaTCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACg < 1:432043/52‑1 (MQ=255) cAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:2055783/43‑1 (MQ=255) cAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAgg < 1:2428334/43‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGaa < 1:3018264/44‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:3276463/70‑1 (MQ=255) aCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGgatgat > 1:3532092/1‑55 (MQ=255) aCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtt < 1:884341/71‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt < 1:3800745/71‑1 (MQ=255) gTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAg > 1:239906/1‑38 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:2852763/71‑1 (MQ=255) gcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1585492/62‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:568395/64‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt < 1:1908208/71‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt > 1:2785062/1‑71 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:243692/1‑70 (MQ=255) aaGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2326430/71‑1 (MQ=255) aGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCg < 1:576963/71‑1 (MQ=255) | GCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTACGGCAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCG > minE/311722‑311859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |