| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,605,504 | A→G | 23.9% | intergenic (‑83/‑308) | uspA ← / → yhiO | universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,605,504 | 0 | A | G | 23.9% | 115.8 / 38.0 | 67 | intergenic (‑83/‑308) | uspA/yhiO | universal stress global response regulator/predicted universal stress (ethanol tolerance) protein B |
| Reads supporting (aligned to +/- strand): ref base A (25/26); new base G (7/9); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
AGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > minE/2605441‑2605573 | aGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTgcgc < 1:3498905/71‑1 (MQ=255) ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgg < 1:1792918/71‑1 (MQ=255) ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:3549497/71‑1 (MQ=255) ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:951110/71‑1 (MQ=255) ttGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCgg < 1:3343757/71‑1 (MQ=255) gTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCggg > 1:1225345/1‑70 (MQ=255) tCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGt < 1:3585/62‑1 (MQ=255) tCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGc < 1:3233630/71‑1 (MQ=255) cGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgggg > 1:1183417/1‑69 (MQ=255) cGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCgggg > 1:92917/1‑69 (MQ=255) gATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCt < 1:1011969/70‑1 (MQ=255) aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTaa > 1:621440/1‑71 (MQ=255) aTGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGTCTaa < 1:2165794/71‑1 (MQ=255) tGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAc > 1:337017/1‑71 (MQ=255) gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:2451709/1‑57 (MQ=255) gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:870586/1‑57 (MQ=255) gACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGt > 1:2031162/1‑57 (MQ=255) aCTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAcc > 1:3744539/1‑70 (MQ=255) cTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCg > 1:823014/1‑54 (MQ=255) tGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCt < 1:3839152/64‑1 (MQ=255) ggCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGc > 1:281144/1‑71 (MQ=255) gCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAg < 1:1956480/69‑1 (MQ=255) cAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGt < 1:3888551/71‑1 (MQ=255) aGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTa < 1:1451055/71‑1 (MQ=255) gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTg < 1:595414/49‑1 (MQ=255) gCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTaa > 1:2958301/1‑60 (MQ=255) cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTa > 1:2673115/1‑69 (MQ=255) cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTaa > 1:2950010/1‑59 (MQ=255) cTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTa > 1:3051232/1‑69 (MQ=255) tAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTa < 1:1603554/57‑1 (MQ=255) aGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGGGAACTGCGt < 1:2951470/45‑1 (MQ=255) gCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGa < 1:1335566/70‑1 (MQ=255) gTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAAccc > 1:2541238/1‑57 (MQ=255) tttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCa < 1:3879602/71‑1 (MQ=255) ttCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAg > 1:1670213/1‑71 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCa > 1:1013183/1‑54 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtg > 1:70330/1‑71 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt < 1:2913864/70‑1 (MQ=255) cTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgt < 1:2605836/70‑1 (MQ=255) gtgCCGTTACGTCACCCGCGTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:1796126/70‑1 (MQ=255) cGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAgtgt < 1:1428150/65‑1 (MQ=255) gTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAcc < 1:852897/71‑1 (MQ=255) ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGtt < 1:3196860/64‑1 (MQ=255) ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAc < 1:2241418/69‑1 (MQ=255) ttACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGAc < 1:886030/69‑1 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGa > 1:635319/1‑66 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:2871342/1‑71 (MQ=255) aCGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:1847495/1‑71 (MQ=255) gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTaaa < 1:3068280/49‑1 (MQ=255) gTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct > 1:3886249/1‑71 (MQ=255) tCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctt < 1:272135/71‑1 (MQ=255) tCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcct > 1:3643296/1‑70 (MQ=255) cACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctta > 1:3523187/1‑71 (MQ=255) cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCAtcctttcctt < 1:2013333/71‑1 (MQ=255) cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctt < 1:2017736/71‑1 (MQ=255) cgcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacct < 1:2937843/70‑1 (MQ=255) gcgTCCTGCGAACTGCGTTCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATccttacctta < 1:496190/71‑1 (MQ=255) gcgTCCTGCGAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATcctta > 1:359422/1‑66 (MQ=255) gcgTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGAt < 1:2820465/45‑1 (MQ=255) cGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAcc > 1:3518070/1‑71 (MQ=255) gAACTGCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGAccc < 1:110906/71‑1 (MQ=255) gAACTACGTGCGCGGGGCTTACCCAGCCGTAAAGATTCAGTGTTAAGACCATc > 1:1993295/1‑53 (MQ=255) gAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTtat > 1:1615210/1‑63 (MQ=255) aCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCgg < 1:1378711/71‑1 (MQ=255) aCTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCg > 1:276241/1‑70 (MQ=255) cTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGga > 1:1309540/1‑71 (MQ=255) tACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:2233535/1‑71 (MQ=255) aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:1856896/1‑70 (MQ=255) aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:2267476/1‑70 (MQ=255) aCGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGgat > 1:1372975/1‑70 (MQ=255) | AGTTGTCGATGACTGGCCAGCTAGCGTTTCTTGTGCCGTTACGTCACCCGCGTCCTGCGAACTACGTGCGCGGGGCTAACCCAGCCGTAAAGATTCAGTGTTAAGACCATCCTTACCTTATAGCGACCCGGAT > minE/2605441‑2605573 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |