| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,608,096 | T→C | 21.0% | intergenic (‑206/+104) | pitA ← / ← nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,608,096 | 0 | T | C | 21.0% | 70.0 / 15.6 | 38 | intergenic (‑206/+104) | pitA/nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Reads supporting (aligned to +/- strand): ref base T (15/15); new base C (3/5); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCC > minE/2608028‑2608162 | tCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTcc > 1:3808374/1‑71 (MQ=255) ttCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Taa < 1:3823549/69‑1 (MQ=255) ttCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGtttt < 1:1140746/64‑1 (MQ=255) tCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTGACGccc > 1:2256533/1‑58 (MQ=255) ccTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag < 1:3135682/70‑1 (MQ=255) cTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGt > 1:3259354/1‑54 (MQ=255) tttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTAACGTCCGt > 1:1213125/1‑56 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAgaggag < 1:1210578/69‑1 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAgc < 1:563309/70‑1 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAgagga < 1:857298/69‑1 (MQ=255) tAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag > 1:1042874/1‑62 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg < 1:2252254/71‑2 (MQ=255) tatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcg < 1:1581266/70‑2 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcgtg > 1:3525982/1‑67 (MQ=255) gATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAgagg < 1:183097/57‑1 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggt > 1:687266/1‑70 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAACGTGGAAAggt > 1:2967704/1‑70 (MQ=255) tGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGttt < 1:1426431/41‑1 (MQ=255) tGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTa > 1:419066/1‑71 (MQ=255) ggTGCGACCACTGAGGAATCTTTACAATTCACGTCCGttt < 1:1555140/40‑1 (MQ=255) gTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTAt < 1:2763547/70‑1 (MQ=255) gACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga < 1:2232496/71‑1 (MQ=255) ccACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga > 1:170219/1‑69 (MQ=255) ctcTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTagagag < 1:3577709/69‑1 (MQ=255) cTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAAc < 1:810081/48‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGaa > 1:2410538/1‑71 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGa > 1:2146907/1‑70 (MQ=255) gAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAAt > 1:2999092/1‑71 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAgaggag < 1:478477/37‑1 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg < 1:1887432/71‑1 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAAATCGGATAGGGTATTTAGAGAGGAATCAg > 1:2107823/1‑71 (MQ=255) aaTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAgg < 1:1628187/71‑1 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:398495/1‑43 (MQ=255) ttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg < 1:126788/65‑1 (MQ=255) tACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg > 1:1249411/1‑64 (MQ=255) aCAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgt > 1:3401170/1‑65 (MQ=255) aaTTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAg > 1:516944/1‑41 (MQ=255) ttCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTgg > 1:2380378/1‑71 (MQ=255) cACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGcc > 1:3325786/1‑71 (MQ=255) aCGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTa < 1:2252844/44‑1 (MQ=255) | TCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCC > minE/2608028‑2608162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |