| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,636,243 | G→A | 10.2% | intergenic (+63/‑35) | ugpB → / → ugpA | glycerol‑3‑phosphate transporter subunit/glycerol‑3‑phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,636,243 | 0 | G | A | 10.2% | 134.8 / 5.6 | 49 | intergenic (+63/‑35) | ugpB/ugpA | glycerol‑3‑phosphate transporter subunit/glycerol‑3‑phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (19/25); new base A (2/3); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
AATCAGTGTAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCGCTGG > minE/2636179‑2636313 | aaTCAGTGTAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATaagcgaagcg > 1:2163439/1‑70 (MQ=255) cAGTGTAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATc > 1:3620971/1‑71 (MQ=255) cAGTGTAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATc > 1:1708105/1‑71 (MQ=255) atgcgacgctggcgcgtcttatcaggcctacacgctgcgatttTGTAGGCCGGATAAGCAAAGCGCATc < 1:11235‑M2/26‑1 (MQ=255) atgcgacgctgacgcgtcttatctggcctacacgctgcgatttTGTAGGCCGGATAAGCAAAGCGCATCCg > 1:1996752‑M2/44‑71 (MQ=255) tAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGca < 1:771700/71‑1 (MQ=255) cgctggcgcgtcttatcaggcctacacgctgcgatttTGTAGGCCGGATAAGCAAAGCGCATCCGgcacg > 1:1249894‑M2/38‑69 (MQ=255) tCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGtt < 1:2897187/71‑1 (MQ=255) gATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAg > 1:3150116/1‑71 (MQ=255) aTGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAgg < 1:1114380/71‑1 (MQ=255) gCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCAt < 1:2670788/54‑1 (MQ=255) gTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAgg < 1:3476419/67‑1 (MQ=255) ttatcaggcctacgcgctgcgatttTGTAGGCCGGATAAGCAAAGCGCAt < 1:2102570‑M2/25‑1 (MQ=255) ttCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCa > 1:3249574/1‑63 (MQ=255) tCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAGGCGCATcc > 1:1044490/1‑51 (MQ=255) tCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAAc > 1:3330253/1‑71 (MQ=255) tCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAAc > 1:3371114/1‑71 (MQ=255) tcaggcctacgcgctgcgatttTGTAGGCCGGATAAGCAAAGCGCa < 1:165201‑M2/24‑1 (MQ=255) cTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCa < 1:445078/59‑1 (MQ=255) cTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGTAATTaa < 1:360485/67‑1 (MQ=255) cTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTaa < 1:1258658/67‑1 (MQ=255) cTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTaa < 1:363219/67‑1 (MQ=255) tATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGaa > 1:2098829/1‑61 (MQ=255) gACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtca > 1:2819246/1‑71 (MQ=255) aCCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtcat > 1:2887644/1‑71 (MQ=255) aCCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtc < 1:2624337/69‑1 (MQ=255) cTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTaa > 1:2069101/1‑57 (MQ=255) cTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtcatca < 1:261595/71‑1 (MQ=255) cTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtcatca < 1:2084227/71‑1 (MQ=255) gCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTcatcat < 1:441376/69‑1 (MQ=255) gCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCatcatc < 1:3188620/70‑1 (MQ=255) aTCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtcat > 1:1123209/1‑64 (MQ=255) aTCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGtcat > 1:1496474/1‑64 (MQ=255) aTCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTcatcat < 1:2103294/67‑1 (MQ=255) gcgTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTaa > 1:856756/1‑49 (MQ=255) gcgTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGt < 1:2579761/53‑1 (MQ=255) gcgTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTaa < 1:3025269/55‑1 (MQ=255) cgTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCg < 1:384229/71‑1 (MQ=255) gtgtAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCg < 1:1624514/70‑1 (MQ=255) gtAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTaa > 1:3090183/1‑51 (MQ=255) gtAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGgtg > 1:2323368/1‑71 (MQ=255) tAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGgtgt < 1:3572628/71‑1 (MQ=255) ggCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGaa < 1:3855948/38‑1 (MQ=255) gCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATg < 1:798182/49‑1 (MQ=255) ccGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCatcatc < 1:3908814/56‑1 (MQ=255) ccGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCg > 1:3599549/1‑71 (MQ=255) cGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCg > 1:898029/1‑70 (MQ=255) cGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGc > 1:725980/1‑71 (MQ=255) ggATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCt < 1:3427387/71‑1 (MQ=255) gATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCgg < 1:1530378/61‑1 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGtca < 1:1040693/41‑1 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGt < 1:899027/39‑1 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCgctgg < 1:143449/71‑1 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCgctgg > 1:3054846/1‑71 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCgctgg > 1:2913820/1‑71 (MQ=255) gaagcgCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCgctgg > 1:2211473/1‑71 (MQ=255) | AATCAGTGTAATGTCGGATGCGTTTCGCTTATCTGACCTGGCATCGCGTGTAGGCCGGATAAGCGAAGCGCATCCGGCACAGTTCAGGAATTAACCGTAATGTCATCATCCCGTCCGGTGTTCCGCTCGCGCTGG > minE/2636179‑2636313 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |