| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,663,990 | G→A | 17.6% | G325E (GGG→GAG) | rtcB → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,663,990 | 0 | G | A | 17.6% | 114.0 / 17.4 | 51 | G325E (GGG→GAG) | rtcB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/27); new base A (6/3); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
TGTCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTAA > minE/2663921‑2664056 | tgtCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTggg > 1:3065008/1‑71 (MQ=255) gtCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGc > 1:195513/1‑71 (MQ=255) tCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGc < 1:983888/70‑1 (MQ=255) cgcgTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTg < 1:1476652/68‑1 (MQ=255) cgTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGaaa < 1:2232928/71‑1 (MQ=255) tGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATg < 1:3204735/71‑1 (MQ=255) tGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATg > 1:437851/1‑68 (MQ=255) ggTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGaa > 1:1871514/1‑69 (MQ=255) gTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAag < 1:2736640/69‑1 (MQ=255) cAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTc > 1:3940286/1‑71 (MQ=255) aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTTGGCTGGGAAATGAAGAGTCg < 1:1027827/71‑1 (MQ=255) aaTATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCg > 1:40308/1‑71 (MQ=255) tatGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGtt < 1:2214856/71‑1 (MQ=255) tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGtt > 1:2191142/1‑69 (MQ=255) tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCt < 1:3360339/71‑1 (MQ=255) tGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCt < 1:3007106/71‑1 (MQ=255) gAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt < 1:2792630/71‑1 (MQ=255) aaTTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt > 1:1388129/1‑70 (MQ=255) ttattCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAag > 1:1940886/1‑57 (MQ=255) ttCCCGCTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt < 1:2603657/65‑1 (MQ=255) cccGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAg > 1:1426356/1‑71 (MQ=255) ccGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCt > 1:3880088/1‑40 (MQ=255) ggTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGcc > 1:2480941/1‑70 (MQ=255) ttCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCa < 1:1269009/69‑1 (MQ=255) tGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt < 1:444547/53‑1 (MQ=255) tGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGt < 1:823017/53‑1 (MQ=255) tGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGcc < 1:1083902/71‑1 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTc > 1:3474021/1‑49 (MQ=255) gggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGt < 1:1812938/67‑1 (MQ=255) ggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTc > 1:2393166/1‑53 (MQ=255) ggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCa > 1:2992674/1‑58 (MQ=255) ggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg < 1:2500493/70‑1 (MQ=255) ggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcg < 1:2982745/70‑1 (MQ=255) aGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcgggc < 1:802019/71‑1 (MQ=255) gCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGc < 1:265659/54‑1 (MQ=255) gCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGc < 1:1382742/54‑1 (MQ=255) gCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCcgggcg < 1:2935270/71‑1 (MQ=255) aaaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGt > 1:1913938/1‑48 (MQ=255) aaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTaa < 1:2249705/71‑1 (MQ=255) aGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAAt > 1:2731358/1‑71 (MQ=255) gCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATg < 1:2819381/71‑1 (MQ=255) tttATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTa < 1:2857186/66‑1 (MQ=255) ttATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGc > 1:2402911/1‑71 (MQ=255) tATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAg > 1:3238509/1‑69 (MQ=255) tATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGcc > 1:2921123/1‑71 (MQ=255) tCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGa < 1:1596499/71‑1 (MQ=255) cGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCg < 1:1195964/69‑1 (MQ=255) gTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAAc > 1:354753/1‑71 (MQ=255) tCCGTTGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACt < 1:440708/71‑1 (MQ=255) cGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTaa < 1:2702686/71‑1 (MQ=255) cGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTa < 1:1203654/70‑1 (MQ=255) gTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTaa > 1:3208119/1‑70 (MQ=255) gggCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTa < 1:3174323/67‑1 (MQ=255) | TGTCTGCGCGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTAA > minE/2663921‑2664056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |