| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,673,919 | C→A | 22.7% | G207C (GGT→TGT) | gntT ← | gluconate transporter, high‑affinity GNT I system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,673,919 | 0 | C | A | 22.7% | 186.8 / 32.5 | 79 | G207C (GGT→TGT) | gntT | gluconate transporter, high‑affinity GNT I system |
| Reads supporting (aligned to +/- strand): ref base C (30/31); new base A (12/6); total (42/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01 | |||||||||||
GAGAGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > minE/2673850‑2673988 | gagagGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCt > 1:949460/1‑71 (MQ=255) ccAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa > 1:3169300/1‑71 (MQ=255) cAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAt < 1:2718459/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt < 1:1040604/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt < 1:2430961/71‑1 (MQ=255) aGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAAtt < 1:3931175/71‑1 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTg > 1:2301252/1‑71 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTg > 1:588030/1‑71 (MQ=255) gACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTg < 1:237913/71‑1 (MQ=255) cGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGc > 1:2394486/1‑71 (MQ=255) gACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGaa < 1:640423/62‑1 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCt > 1:2982773/1‑67 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATc > 1:886173/1‑71 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATc > 1:519016/1‑71 (MQ=255) aCGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATc < 1:2402020/71‑1 (MQ=255) gCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:1553719/1‑71 (MQ=255) ccAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGAt < 1:1452475/71‑1 (MQ=255) cAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATa > 1:3726731/1‑71 (MQ=255) aaaGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAc > 1:2615375/1‑71 (MQ=255) aGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCt > 1:3778163/1‑71 (MQ=255) aGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCt > 1:3002204/1‑71 (MQ=255) tCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAc < 1:713600/66‑1 (MQ=255) cGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTc > 1:174264/1‑70 (MQ=255) cGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTg > 1:3243236/1‑53 (MQ=255) ggCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCaa > 1:3830473/1‑71 (MQ=255) ggCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTc < 1:3598098/69‑1 (MQ=255) gCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAcc < 1:382656/64‑1 (MQ=255) gCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCtt > 1:3604472/1‑66 (MQ=255) aTCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAacac < 1:2067347/71‑1 (MQ=255) aTCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAaca > 1:1488750/1‑70 (MQ=255) tctcTTCTTCGCTGAAGGTTTTCGGGCTGTAGAGACCt < 1:1077829/38‑1 (MQ=255) ctctTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTcc > 1:1871778/1‑40 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGaa > 1:158786/1‑43 (MQ=255) tcttctTCGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGaa > 1:691040/1‑43 (MQ=255) cttcttCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAac < 1:2778028/65‑1 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACAcgc < 1:1390412/68‑1 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAg > 1:2560202/1‑71 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACATTcc > 1:147886/1‑37 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACATTc > 1:3666942/1‑36 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAg > 1:1717567/1‑71 (MQ=255) ttcttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAg > 1:3947897/1‑71 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGATCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGa < 1:956127/68‑1 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATAcc > 1:2824383/1‑55 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGa < 1:800900/68‑1 (MQ=255) cttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCg > 1:1582130/1‑71 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGa < 1:3242546/37‑1 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGa < 1:1071385/37‑1 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGa < 1:1985031/37‑1 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTa > 1:1567936/1‑46 (MQ=255) ttcGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTa > 1:3394083/1‑46 (MQ=255) cGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTa > 1:2214262/1‑70 (MQ=255) cGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTa < 1:1709633/70‑1 (MQ=255) cGCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGt < 1:3598677/69‑1 (MQ=255) cGCTGAAGGTTTACGCGCTGTAGAGACCTTCCGGAATTGGCt > 1:2356120/1‑42 (MQ=255) gCTGAAGGTTTTCGCGCTGTAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTaaa < 1:1483020/71‑1 (MQ=255) aaGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccgg < 1:1184811/71‑1 (MQ=255) aaGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccgg < 1:3622796/71‑1 (MQ=255) aaGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccg < 1:1256990/70‑1 (MQ=255) ttttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggaccg > 1:1702798/1‑71 (MQ=255) ttttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggaccg < 1:2910006/71‑1 (MQ=255) tttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGa > 1:2170510/1‑38 (MQ=255) ttCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggaccgg > 1:3714387/1‑70 (MQ=255) tCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc < 1:1349680/70‑1 (MQ=255) tCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc < 1:198360/70‑1 (MQ=255) tCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGc < 1:869428/70‑1 (MQ=255) cgcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCgaga < 1:3681465/71‑1 (MQ=255) cgcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCgaga < 1:3042338/71‑1 (MQ=255) gcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTa > 1:413615/1‑54 (MQ=255) gcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGaa < 1:989437/71‑1 (MQ=255) gcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGaa > 1:178030/1‑71 (MQ=255) gcTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGaa < 1:2630798/71‑1 (MQ=255) tGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAAt > 1:3641594/1‑70 (MQ=255) tAGAGACATTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATc < 1:6605/69‑1 (MQ=255) gaCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTc < 1:2196042/71‑1 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAaccggacc > 1:877193/1‑54 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCg > 1:473979/1‑71 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCg > 1:392403/1‑71 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCg < 1:1882776/71‑1 (MQ=255) aCCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCg > 1:3605153/1‑71 (MQ=255) ccTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCgg < 1:1014066/71‑1 (MQ=255) | GAGAGGTCCAGACGCTGACGCCAAAGCTCGGCATCTCTTCTTCGCTGAAGGTTTTCGCGCTGTAGAGACCTTCCGGAATTGGCTTATCGATACCTTTCAACACGCGAGCGTAAACCGGACCGGCGAGAATCACGGTCGG > minE/2673850‑2673988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |