Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,365	T→A	7.8%	M189L (ATG→TTG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,365	0	T	A	7.8%	254.7 / 11.3	90	M189L (ATG→TTG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand):  ref base T (45/38);  new base A (4/3);  total (49/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCA  >  minE/2677295‑2677435                                                                       |                                                                       aGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt                                                                        <  1:3508478/71‑1 (MQ=255) aGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt                                                                        <  1:328488/71‑1 (MQ=255)   cccTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt                                                                        >  1:2548791/1‑69 (MQ=255)   cccTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt                                                                        >  1:3544227/1‑69 (MQ=255)      tttGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGt                                                                   >  1:2225561/1‑71 (MQ=255)        tGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg                                                                 >  1:2652157/1‑71 (MQ=255)        tGATTTGTCTGTCATTAGCGTACCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcg                                                                 >  1:3460570/1‑71 (MQ=255)          aTTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTcgcg                                                               <  1:1070768/71‑1 (MQ=255)            ttGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGt                                                              >  1:2785377/1‑70 (MQ=255)             tgtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGtt                                                             >  1:2247712/1‑70 (MQ=255)              gtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTg                                                            <  1:3826493/70‑1 (MQ=255)              gtctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTg                                                            <  1:3830991/70‑1 (MQ=255)               tctgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCAt                                                                        <  1:372809/57‑1 (MQ=255)                 tgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACg                                                         >  1:1207380/1‑70 (MQ=255)                 tgtcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACg                                                         >  1:2071921/1‑70 (MQ=255)                   tcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGa                                                        <  1:2272721/69‑1 (MQ=255)                   tcATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAat                                                      <  1:452830/71‑1 (MQ=255)                      ttAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATg                                                   <  1:3720596/71‑1 (MQ=255)                        aGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGt                                                  <  1:289529/70‑1 (MQ=255)                         gCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATc                                                                       >  1:3371902/1‑48 (MQ=255)                         gCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGtt                                                 <  1:1957323/70‑1 (MQ=255)                           gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                              <  1:3890914/71‑1 (MQ=255)                           gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                              <  1:3886274/71‑1 (MQ=255)                           gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                              <  1:1832287/71‑1 (MQ=255)                           gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                              <  1:2824569/71‑1 (MQ=255)                             aTCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt                                                <  1:3770208/67‑1 (MQ=255)                             aTCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt                                                >  1:2200781/1‑67 (MQ=255)                              tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                           >  1:1232981/1‑71 (MQ=255)                              tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                            <  1:511991/70‑1 (MQ=255)                                 actactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAg                                                                     <  1:1519871/42‑1 (MQ=255)                                  ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                           >  1:1417220/1‑67 (MQ=255)                                  ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                       >  1:2723191/1‑71 (MQ=255)                                   tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGAc                                                          >  1:930502/1‑51 (MQ=255)                                   tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                      >  1:632769/1‑71 (MQ=255)                                   tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                      >  1:447278/1‑71 (MQ=255)                                   tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTAGACGAATATGTTTTTGAATTa                                        >  1:3738356/1‑69 (MQ=255)                                     ctTAATAAGCAGGCCATTTGCTCTACCAGCCCCATc                                                                       >  1:2592964/1‑36 (MQ=255)                                        aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                         <  1:1032001/63‑1 (MQ=255)                                        aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                   <  1:294496/69‑1 (MQ=255)                                        aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                   <  1:1573181/69‑1 (MQ=255)                                         ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                   <  1:620420/68‑1 (MQ=255)                                          taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt                                                >  1:1955693/1‑54 (MQ=255)                                          taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttt                                                >  1:2528655/1‑54 (MQ=255)                                          taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                 >  1:1006126/1‑69 (MQ=255)                                          taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                 >  1:1400013/1‑69 (MQ=255)                                            aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCagcagc                             >  1:659153/1‑71 (MQ=255)                                               aGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                           >  1:2557141/1‑70 (MQ=255)                                                ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                         <  1:1225575/71‑1 (MQ=255)                                                ggCCATTTGCTATACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                         >  1:2141876/1‑71 (MQ=255)                                                 gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagca                               <  1:2668072/64‑1 (MQ=255)                                                 gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                         >  1:2080422/1‑70 (MQ=255)                                                 gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                         >  1:2771993/1‑70 (MQ=255)                                                  ccATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGaa                                                       <  1:1324491/39‑1 (MQ=255)                                                   cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                         <  1:570166/52‑1 (MQ=255)                                                   cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                      >  1:3814505/1‑71 (MQ=255)                                                    aTTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAatat                                                    <  1:1439093/40‑1 (MQ=255)                                                     tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                          <  1:3856267/49‑1 (MQ=255)                                                     tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                          <  1:1020589/49‑1 (MQ=255)                                                     tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                    <  1:2162599/71‑1 (MQ=255)                                                     tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                    <  1:3210283/71‑1 (MQ=255)                                                      ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                   >  1:2944899/1‑71 (MQ=255)                                                         ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGt                <  1:700351/71‑1 (MQ=255)                                                          tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                             <  1:1340377/41‑1 (MQ=255)                                                          tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                   <  1:666806/51‑1 (MQ=255)                                                           ctACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                              >  1:2213496/1‑39 (MQ=255)                                                           ctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                       >  1:2489470/1‑46 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                   >  1:694205/1‑65 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct             >  1:3896256/1‑71 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct             >  1:1822442/1‑71 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct             >  1:2217154/1‑71 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct             >  1:99037/1‑71 (MQ=255)                                                            tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc              >  1:1251752/1‑70 (MQ=255)                                                              ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg            >  1:2229720/1‑70 (MQ=255)                                                              ccAGCCCCATCAGGTCGCGTTGACGAATATGATTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg            >  1:3393685/1‑70 (MQ=255)                                                               cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa          <  1:3872632/71‑1 (MQ=255)                                                                aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                  >  1:221171/1‑62 (MQ=255)                                                                aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt         >  1:269866/1‑71 (MQ=255)                                                                aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt         >  1:418647/1‑71 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                         <  1:1573021/38‑1 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg               <  1:229142/64‑1 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt         >  1:1292942/1‑70 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg        <  1:2960844/71‑1 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg        <  1:3542177/71‑1 (MQ=255)                                                                 gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg        <  1:2803257/71‑1 (MQ=255)                                                                  tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAtaa       <  1:3508644/70‑3 (MQ=255)                                                                  ccccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa       >  1:3398659/1‑71 (MQ=255)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                       >  1:2166678/1‑37 (MQ=21)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                       >  1:22136/1‑37 (MQ=21)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                       >  1:1909450/1‑37 (MQ=21)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                       >  1:1024970/1‑37 (MQ=21)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTcc                                   <  1:605972/41‑1 (MQ=25)                                                                    ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtc     <  1:1252999/71‑2 (MQ=255)                                                                     cATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAttt    >  1:3907307/1‑71 (MQ=255)                                                                     cATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt     >  1:1785837/1‑70 (MQ=255)                                                                      aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc   <  1:2651186/71‑1 (MQ=255)                                                                       tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag                              >  1:1485487/1‑43 (MQ=255)                                                                       tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                    <  1:1651933/53‑1 (MQ=255)                                                                       tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca  >  1:430257/1‑71 (MQ=255)                                                                       |                                                                       AGCCCTTTGATTTGTCTGTCATTAGCGTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCA  >  minE/2677295‑2677435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑