BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,677,390	T→C	13.0%	Q180Q (CAA→CAG)	yhgA ←	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,677,390	0	T	C	13.0%	238.8 / 23.4	92	Q180Q (CAA→CAG)	yhgA	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (41/39); new base C (5/7); total (46/46)
Fisher's exact test for biased strand distribution p-value = 7.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 103 total aligned reads displayed.

GTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAA  >  minE/2677321‑2677460

gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                       <  1:1832287/71‑1 (MQ=255)

gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                       <  1:2824569/71‑1 (MQ=255)

gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                       <  1:3890914/71‑1 (MQ=255)

gTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                       <  1:3886274/71‑1 (MQ=255)

   tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                    >  1:1232981/1‑71 (MQ=255)

   tCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGa                                                                     <  1:511991/70‑1 (MQ=255)

       ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGaa                                                                    >  1:1417220/1‑67 (MQ=255)

       ctactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                                                >  1:2723191/1‑71 (MQ=255)

        tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                                               >  1:632769/1‑71 (MQ=255)

        tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAAc                                                               >  1:447278/1‑71 (MQ=255)

        tactTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTAGACGAATATGTTTTTGAATTa                                                                 >  1:3738356/1‑69 (MQ=255)

             aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                  <  1:1032001/63‑1 (MQ=255)

             aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                            <  1:294496/69‑1 (MQ=255)

             aataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                            <  1:1573181/69‑1 (MQ=255)

              ataaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                            <  1:620420/68‑1 (MQ=255)

               taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                          >  1:1006126/1‑69 (MQ=255)

               taaGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcag                                                          >  1:1400013/1‑69 (MQ=255)

                 aGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCagcagc                                                      >  1:659153/1‑71 (MQ=255)

                    aGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                    >  1:2557141/1‑70 (MQ=255)

                     ggCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                  <  1:1225575/71‑1 (MQ=255)

                     ggCCATTTGCTATACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                  >  1:2141876/1‑71 (MQ=255)

                      gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagca                                                        <  1:2668072/64‑1 (MQ=255)

                      gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                  >  1:2771993/1‑70 (MQ=255)

                      gCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCa                                                  >  1:2080422/1‑70 (MQ=255)

                        cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                  <  1:570166/52‑1 (MQ=255)

                        cATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATAc                                               >  1:3814505/1‑71 (MQ=255)

                          tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                   <  1:3856267/49‑1 (MQ=255)

                          tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAt                                                                   <  1:1020589/49‑1 (MQ=255)

                          tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                             <  1:2162599/71‑1 (MQ=255)

                          tttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                             <  1:3210283/71‑1 (MQ=255)

                           ttGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                            >  1:2944899/1‑71 (MQ=255)

                              ctctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGt                                         <  1:700351/71‑1 (MQ=255)

                               tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTg                                                                      <  1:1340377/41‑1 (MQ=255)

                               tctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTcc                                                            <  1:666806/51‑1 (MQ=255)

                                ctACCAGCCCCATCAGGTCGCGTTGACGAATATGttttt                                                                       >  1:2213496/1‑39 (MQ=255)

                                ctACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTaa                                                                >  1:2489470/1‑46 (MQ=255)

                                 tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTc                                            >  1:694205/1‑65 (MQ=255)

                                 tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct                                      >  1:1822442/1‑71 (MQ=255)

                                 tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct                                      >  1:3896256/1‑71 (MQ=255)

                                 tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct                                      >  1:2217154/1‑71 (MQ=255)

                                 tACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgc                                       >  1:1251752/1‑70 (MQ=255)

                                   ccAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                     >  1:2229720/1‑70 (MQ=255)

                                   ccAGCCCCATCAGGTCGCGTTGACGAATATGATTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgctg                                     >  1:3393685/1‑70 (MQ=255)

                                    cAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCa                                   <  1:3872632/71‑1 (MQ=255)

                                     aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCg                                           >  1:221171/1‑62 (MQ=255)

                                     aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                  >  1:418647/1‑71 (MQ=255)

                                     aGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                  >  1:269866/1‑71 (MQ=255)

                                      gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAAtt                                                                  <  1:1573021/38‑1 (MQ=255)

                                      gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtg                                        <  1:229142/64‑1 (MQ=255)

                                      gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCAt                                  >  1:1292942/1‑70 (MQ=255)

                                      gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                 <  1:2960844/71‑1 (MQ=255)

                                      gCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATg                                 <  1:2803257/71‑1 (MQ=255)

                                       tcccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCAtaa                                <  1:3508644/70‑3 (MQ=255)

                                       ccccATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGa                                >  1:3398659/1‑71 (MQ=255)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                                >  1:1909450/1‑37 (MQ=21)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                                >  1:1024970/1‑37 (MQ=21)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                                >  1:2166678/1‑37 (MQ=21)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTaa                                                                >  1:22136/1‑37 (MQ=21)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTcc                                                            <  1:605972/41‑1 (MQ=25)

                                         ccAACAGATCGCGCTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtc                              <  1:1252999/71‑2 (MQ=255)

                                          cATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAttt                             >  1:3907307/1‑71 (MQ=255)

                                          cATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAtt                              >  1:1785837/1‑70 (MQ=255)

                                           aTCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtc                            <  1:2651186/71‑1 (MQ=255)

                                            tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCcagcag                                                       >  1:1485487/1‑43 (MQ=255)

                                            tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGt                                             <  1:1651933/53‑1 (MQ=255)

                                            tCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                           >  1:430257/1‑71 (MQ=255)

                                              aGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                          <  1:3591437/70‑1 (MQ=255)

                                                 tCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct                                      >  1:1834015/1‑55 (MQ=255)

                                                   gcgcTGACGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAAtctc                            >  1:1056936/1‑63 (MQ=255)

                                                   gcgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAg                    >  1:564050/1‑71 (MQ=255)

                                                    cgTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAgg                   <  1:2194101/71‑1 (MQ=255)

                                                     gTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcat                          >  1:878175/1‑63 (MQ=255)

                                                     gTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtca                           <  1:1240339/62‑1 (MQ=255)

                                                      ttGAGGAATATGTTTTTGAATTAACTCCAGCAgcgc                                                    <  1:3678936/36‑1 (MQ=255)

                                                        gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGtgct                                      >  1:640683/1‑48 (MQ=255)

                                                        gACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                >  1:224394/1‑70 (MQ=255)

                                                         aCGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa              <  1:219163/71‑1 (MQ=255)

                                                         aCGAATATGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCacc               <  1:1796969/70‑3 (MQ=255)

                                                           gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatca                        <  1:3860157/59‑1 (MQ=255)

                                                           gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatca                        <  1:3189829/59‑1 (MQ=255)

                                                           gAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa              >  1:2966083/1‑69 (MQ=255)

                                                            aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACgg           <  1:2968327/71‑1 (MQ=255)

                                                            aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACgg           <  1:418885/71‑1 (MQ=255)

                                                            aaTATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACgg           <  1:190843/71‑1 (MQ=255)

                                                              tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatca                        >  1:2373093/1‑56 (MQ=255)

                                                              tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACgg           <  1:1086455/69‑1 (MQ=255)

                                                              tatGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTg         <  1:1601212/71‑1 (MQ=255)

                                                              tatGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCTGCACCACGGta         <  1:1200709/71‑2 (MQ=255)

                                                               atGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatcat                       <  1:1737377/56‑1 (MQ=255)

                                                                tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGAt                               <  1:3559349/47‑1 (MQ=255)

                                                                tGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCACCGTCATGCCtgatgatga        <  1:3871552/70‑1 (MQ=25)

                                                                tGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGtaat       <  1:704824/71‑4 (MQ=255)

                                                                tGTTTCTGAATTAACTCCAACAGCGCCATTTTGCGGTGTTGCATAATCTCGTCATCCGGCACCACGGtaat       <  1:2468347/71‑4 (MQ=255)

                                                                 gTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACa      >  1:2046712/1‑71 (MQ=255)

                                                                  tttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat     >  1:1638542/1‑71 (MQ=255)

                                                                   ttttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACat     >  1:872287/1‑70 (MQ=255)

                                                                     ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTtcatca                        >  1:1941061/1‑49 (MQ=255)

                                                                     ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCAt                >  1:3762245/1‑57 (MQ=255)

                                                                     ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGa              >  1:2673901/1‑59 (MQ=255)

                                                                     ttGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACatcaa  >  1:556309/1‑71 (MQ=255)

GTATCCACTACTTAATAAGCAGGCCATTTGCTCTACCAGCCCCATCAGGTCGCGTTGACGAATATGTTTTTGAATTAACTCCAGCAGCGCCATACGTCGGTGCTGCATGATTTCATCATCAGGCATGACGGTGACATCAA  >  minE/2677321‑2677460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`