| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,708,913 | G→A | 49.4% | E54E (GAG→GAA) | yhfZ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,708,913 | 0 | G | A | 49.4% | 1.0 / 110.2 | 79 | E54E (GAG→GAA) | yhfZ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (18/22); new base A (19/20); total (37/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
CATAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGAAGA > minE/2708844‑2708983 | cATAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGt < 1:3616313/71‑1 (MQ=255) aGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATc > 1:2049760/1‑69 (MQ=255) aTGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGc < 1:3951921/71‑1 (MQ=255) tGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACTCAGGCCGCGTTGAAAACGCTGGAATCAAGCg < 1:2017810/71‑1 (MQ=255) tGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCg > 1:2101386/1‑71 (MQ=255) aGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCgg < 1:2854730/70‑1 (MQ=255) gCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAg > 1:977235/1‑71 (MQ=255) tGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGc > 1:2000076/1‑70 (MQ=255) tGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGc > 1:3625986/1‑70 (MQ=255) tGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGc > 1:3211219/1‑70 (MQ=255) gCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGAt < 1:102260/71‑1 (MQ=255) cAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATa > 1:2309048/1‑71 (MQ=255) aaaTGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGa > 1:604660/1‑64 (MQ=255) aaaTGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATAc > 1:1829160/1‑71 (MQ=255) aaaTGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGa < 1:1124701/64‑1 (MQ=255) tgaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGa > 1:1259597/1‑71 (MQ=255) gaatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGc < 1:1312312/62‑1 (MQ=255) gaatgCCGTTCATCCGTTGGCCTGACCCAGGCCGCGTTGAAAACGCTGGAATCAAGCg > 1:95530/1‑58 (MQ=255) aatgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCg < 1:1784948/57‑1 (MQ=255) atgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTg < 1:398286/71‑1 (MQ=255) atgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTg < 1:3847989/71‑1 (MQ=255) atgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTg < 1:1582384/71‑1 (MQ=255) tgCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTg > 1:2526555/1‑70 (MQ=255) ccGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGaa < 1:2199002/70‑1 (MQ=255) gctCATCTGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGAtt < 1:2033570/63‑1 (MQ=255) gTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATc > 1:2366586/1‑46 (MQ=255) ttCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGcc < 1:292496/71‑1 (MQ=255) ttCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATa < 1:648139/58‑1 (MQ=255) tCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACgg < 1:3686968/60‑1 (MQ=255) cATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt > 1:165308/1‑71 (MQ=255) aTCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTg < 1:1098904/71‑1 (MQ=255) aTCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt < 1:2135641/70‑1 (MQ=255) aTCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGt < 1:2617878/70‑1 (MQ=255) ccGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTggg > 1:772264/1‑71 (MQ=255) ccGTTGGCCTGACGCAGGCCGAGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTggg > 1:3771743/1‑71 (MQ=255) cGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTggg < 1:3143876/70‑1 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGaa > 1:1756631/1‑59 (MQ=255) ttGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGgcgc < 1:3705707/71‑1 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:2745416/1‑71 (MQ=255) ggCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCaa > 1:1914000/1‑71 (MQ=255) tGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:3718821/1‑70 (MQ=255) gACGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:1474434/1‑69 (MQ=255) gACGCAAGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATgg > 1:512586/1‑69 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGc < 1:2554354/69‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGc < 1:2312550/69‑1 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAg > 1:3191257/1‑71 (MQ=255) aCGCAGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCg > 1:3916229/1‑56 (MQ=255) gCAGGCCGCGTCGAAAACGCTGAAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt < 1:1024277/70‑1 (MQ=255) cAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGt < 1:1017076/69‑1 (MQ=255) aGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCg < 1:2847687/52‑1 (MQ=255) aGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTAt < 1:2863638/71‑1 (MQ=255) aGGCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTa > 1:1067459/1‑70 (MQ=255) ggCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATc < 1:3046734/71‑1 (MQ=255) gCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCt > 1:2076846/1‑71 (MQ=255) gCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCt > 1:732660/1‑71 (MQ=255) gCCGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCt < 1:2079903/71‑1 (MQ=255) ccGCGTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAg < 1:3006510/64‑1 (MQ=255) cgcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg < 1:1557632/71‑1 (MQ=255) cgcgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTgg < 1:1461942/71‑1 (MQ=255) gcgTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTggg < 1:1377665/51‑1 (MQ=255) cgTTGAAAACGCTGGAATCAAGCGGAGCGATACGGATTg < 1:2122340/39‑1 (MQ=255) ttGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCa < 1:955089/58‑1 (MQ=255) ttGAAAACGCTGGAATCAAGCGGAGCGATACGGAtt < 1:3466584/36‑1 (MQ=255) ttGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGc < 1:740281/57‑1 (MQ=255) ttGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTc > 1:1934384/1‑69 (MQ=255) tGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg > 1:571965/1‑71 (MQ=255) tGAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTg > 1:3545336/1‑65 (MQ=255) gAAAACGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAg < 1:165783/70‑1 (MQ=255) aaaaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgat < 1:1295086/71‑3 (MQ=255) aaaaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgat < 1:1944368/71‑3 (MQ=255) aaCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGaa > 1:3938680/1‑70 (MQ=255) aaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTgg > 1:867698/1‑42 (MQ=255) aaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATg > 1:570401/1‑50 (MQ=255) aaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATg > 1:2912391/1‑50 (MQ=255) aaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgatg > 1:314199/1‑67 (MQ=255) aaCGCTGGAATCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAgatg > 1:3496791/1‑67 (MQ=255) aCGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAat > 1:897188/1‑70 (MQ=255) gCTGGAGTCAAGCGGAGCGTTACGGTTTGAACGCCg < 1:987234/36‑1 (MQ=38) gAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGaa < 1:571382/71‑1 (MQ=255) gTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGAAGa < 1:3654814/71‑1 (MQ=255) | CATAGATGAGCTGGCAAATGAATGCCGTTCATCCGTTGGCCTGACGCAGGCCGCGTTGAAAACGCTGGAGTCAAGCGGAGCGATACGGATTGAACGCCGTGGGCGCAATGGCAGTTATCTGGTCGAGGAATATACGAAGA > minE/2708844‑2708983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |