| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,747,793 | T→C | 14.7% | I103T (ATC→ACC) | rplO → | 50S ribosomal subunit protein L15 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,747,793 | 0 | T | C | 14.7% | 180.4 / 21.5 | 75 | I103T (ATC→ACC) | rplO | 50S ribosomal subunit protein L15 |
| Reads supporting (aligned to +/- strand): ref base T (32/32); new base C (2/9); total (34/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.80e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CCTGGCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGTGGCCTGCG > minE/2747728‑2747862 | ccTGGCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATc > 1:239432/1‑67 (MQ=255) cTGGCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATc > 1:486673/1‑66 (MQ=255) ggCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATc > 1:2136845/1‑70 (MQ=255) ggCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATc > 1:1670129/1‑70 (MQ=255) gCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGa < 1:1206183/71‑1 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGt < 1:904558/69‑1 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGt < 1:312703/69‑1 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTc > 1:118427/1‑71 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTc > 1:623214/1‑71 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCAGATCGAGt < 1:303240/69‑1 (MQ=255) aaGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCAGATCGAGt < 1:3286505/69‑1 (MQ=255) tAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGt < 1:3325084/66‑1 (MQ=255) tAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTcgcg < 1:492644/71‑1 (MQ=255) gAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaa < 1:623841/71‑1 (MQ=255) gAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGa < 1:2154339/70‑1 (MQ=255) aaGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaaa < 1:722342/71‑1 (MQ=255) ggCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGt > 1:1479299/1‑71 (MQ=255) ggCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGt < 1:133117/71‑1 (MQ=255) ggCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCa < 1:42025/53‑1 (MQ=255) ggCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCa < 1:1967081/53‑1 (MQ=255) tcGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATc < 1:294420/55‑1 (MQ=255) gCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCAGATCGAGTTCGCGAAAGt > 1:298118/1‑70 (MQ=255) ggTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGAt < 1:3933748/71‑1 (MQ=255) tgtAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGa < 1:3119482/62‑1 (MQ=255) tgtAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTACCCAGATCGa < 1:2935188/54‑1 (MQ=255) tagtagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaaa > 1:2253276/1‑62 (MQ=255) tagtagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCctg > 1:374336/1‑71 (MQ=255) gtagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCctggc < 1:2826717/71‑1 (MQ=255) gtagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCctggc < 1:215569/71‑1 (MQ=255) tagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTAt < 1:2869646/40‑1 (MQ=255) tagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCGAGATCGAGTTcg < 1:221649/54‑1 (MQ=255) tagACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCctggc < 1:2762356/70‑1 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCctggctgg > 1:877494/1‑69 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCtggctggc > 1:615620/1‑70 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCtggctggc > 1:3792270/1‑70 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCtggctggc > 1:3055467/1‑70 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCg < 1:3470962/71‑1 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCg < 1:1714685/71‑1 (MQ=255) ccTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCg < 1:1869568/71‑1 (MQ=255) cTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGAt > 1:3217629/1‑41 (MQ=255) tGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTcgcg < 1:1333043/50‑1 (MQ=255) gAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGa < 1:1160736/50‑1 (MQ=255) gAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAg > 1:1371193/1‑71 (MQ=255) aaCACGCTGAAAGCGGCTAACATTATCGGTACCCAGATCGAGTTCGCGaaa > 1:3726816/1‑51 (MQ=255) acacGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTa < 1:1648609/71‑1 (MQ=255) cacGCTGAAAGCGGCTAACATTATCGGTATCCAGATc > 1:2987342/1‑37 (MQ=255) cacGCTGAAAGCGGCTAACATTATCGGTATCCAGATc > 1:3715212/1‑37 (MQ=255) acGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAAc < 1:1796750/71‑1 (MQ=255) acGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAAc < 1:1463025/71‑1 (MQ=255) gCTGAAAGCGGCTAACATTATCGGTACCCAGATCGAGtt < 1:1092495/39‑1 (MQ=255) cTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaaa > 1:1458812/1‑45 (MQ=255) cTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaaa > 1:3407222/1‑45 (MQ=255) cTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGaaa > 1:2078544/1‑45 (MQ=255) cTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGaa > 1:2860689/1‑63 (MQ=255) gAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGAc > 1:2976228/1‑69 (MQ=255) aaaGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGt < 1:1603565/62‑1 (MQ=255) aaGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCg < 1:1201713/71‑1 (MQ=255) aGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTaa > 1:1229657/1‑62 (MQ=255) gCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGt < 1:2949329/71‑1 (MQ=255) gCGGCTAACATTATCGGTACCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGt < 1:3013451/71‑1 (MQ=255) ggCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTaa > 1:3465994/1‑71 (MQ=255) ggCTAACATTATCGGTACCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTaa < 1:519194/71‑1 (MQ=255) gCTAACATTATCGGTACCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTaa < 1:1777567/70‑1 (MQ=255) tAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTaa > 1:2669473/1‑68 (MQ=255) aaCATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGt > 1:1096887/1‑71 (MQ=255) aaCATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGt > 1:178238/1‑71 (MQ=255) cATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATc < 1:3926036/37‑1 (MQ=255) cATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTc < 1:2406771/71‑1 (MQ=255) aTTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCgg > 1:1115998/1‑61 (MQ=255) ttATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCg < 1:2723071/70‑1 (MQ=255) ttATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGt < 1:2668757/71‑1 (MQ=255) tATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAAc > 1:570453/1‑51 (MQ=255) gTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGt < 1:2542835/61‑1 (MQ=255) aTCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGTGGCCTGc > 1:1935109/1‑70 (MQ=255) aTCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGTGGCCTGCg > 1:3309554/1‑71 (MQ=255) aTCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGTGGCCTGCg > 1:1772614/1‑71 (MQ=255) | CCTGGCTAAAGTAGAAGGCGGTGTAGTAGACCTGAACACGCTGAAAGCGGCTAACATTATCGGTATCCAGATCGAGTTCGCGAAAGTGATCCTGGCTGGCGAAGTAACGACTCCGGTAACTGTTCGTGGCCTGCG > minE/2747728‑2747862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |