| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,749,714 | A→T | 6.1% | D54V (GAC→GTC) | rpsM → | 30S ribosomal subunit protein S13 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,749,714 | 0 | A | T | 6.1% | 313.7 / 6.8 | 110 | D54V (GAC→GTC) | rpsM | 30S ribosomal subunit protein S13 |
| Reads supporting (aligned to +/- strand): ref base A (52/51); new base T (2/5); total (54/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 115 total aligned reads displayed.
AAGCCATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCATC > minE/2749645‑2749784 | aaGCCATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGa < 1:3468486/70‑1 (MQ=255) gCCATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGaca > 1:706859/1‑70 (MQ=255) cATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCCGTGAGCTGTCTGAAGGACAAATCGACACg < 1:2072679/70‑1 (MQ=255) cATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGc > 1:2335944/1‑71 (MQ=255) cTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGc < 1:2094434/70‑1 (MQ=255) ggCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGa > 1:3645370/1‑60 (MQ=255) ggCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGTCACGCAGCGTg > 1:573026/1‑71 (MQ=255) ggCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGTCACGCAGCGTg > 1:1966625/1‑71 (MQ=255) ggCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGt > 1:71163/1‑70 (MQ=255) ggCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGt > 1:3881014/1‑70 (MQ=255) cTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGTCACGCAGCGTgtc < 1:288538/71‑3 (MQ=255) tGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGTCACGCAGCGTgtc < 1:2833846/70‑3 (MQ=255) gCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg > 1:2418718/1‑70 (MQ=255) gCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg > 1:2414071/1‑70 (MQ=255) cAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg < 1:3240823/69‑1 (MQ=255) cAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg > 1:2837355/1‑69 (MQ=255) cAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg < 1:2148973/69‑1 (MQ=255) cAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACg < 1:711544/69‑1 (MQ=255) aGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATGGACACGCTGCGTGACGAAg < 1:3019186/71‑1 (MQ=255) aGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTTACGAAg < 1:5021/71‑1 (MQ=255) aGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:2809577/71‑1 (MQ=255) aGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:2646292/71‑1 (MQ=255) aGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:428194/71‑1 (MQ=255) gCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg > 1:1717313/1‑70 (MQ=255) gCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGt > 1:1122014/1‑71 (MQ=255) cGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGtt > 1:3830955/1‑71 (MQ=255) cGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGtt < 1:1582229/71‑1 (MQ=255) cGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGtt < 1:3613400/71‑1 (MQ=255) cGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGtt < 1:815326/71‑1 (MQ=255) aTCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGa > 1:1230098/1‑59 (MQ=255) aTCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGa > 1:3168334/1‑59 (MQ=255) aTCGATGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTg > 1:3736098/1‑54 (MQ=255) tCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCaa < 1:689771/70‑1 (MQ=255) tCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCaa < 1:3374609/70‑1 (MQ=255) gCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAAtt > 1:1517959/1‑71 (MQ=255) cTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTg < 1:829069/50‑1 (MQ=255) cTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGtt > 1:2679075/1‑62 (MQ=255) cTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAAttt < 1:1952777/71‑1 (MQ=255) gAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGa > 1:2082643/1‑41 (MQ=255) gAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:1173689/1‑71 (MQ=255) gAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:1071050/1‑71 (MQ=255) gAAGATGATAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACg > 1:3866869/1‑45 (MQ=255) aaGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTg < 1:3325803/69‑1 (MQ=255) aaGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTg < 1:151582/69‑1 (MQ=255) aGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTc < 1:1606536/70‑1 (MQ=255) aGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCg > 1:3272576/1‑71 (MQ=255) gATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:961603/1‑68 (MQ=255) gATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:3846610/1‑68 (MQ=255) gATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:3851132/1‑68 (MQ=255) aTGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGTCACGCAGCGTg < 1:2837524/48‑1 (MQ=39) gTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:2079273/52‑1 (MQ=255) gTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:649664/52‑1 (MQ=255) gTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:2169087/52‑1 (MQ=255) tAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAg > 1:1246784/1‑71 (MQ=255) aaGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAgg > 1:85942/1‑71 (MQ=255) aaGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAgg > 1:3140731/1‑71 (MQ=255) aGATCAGTGAGCTGTCTGAAGGACAAATCGACACg > 1:29678/1‑35 (MQ=255) aGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGt > 1:3354351/1‑64 (MQ=255) aGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGt > 1:213383/1‑71 (MQ=255) tCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGt < 1:3151476/46‑1 (MQ=255) cAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAg < 1:1491284/44‑1 (MQ=255) cAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATc > 1:207782/1‑71 (MQ=255) aGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCt > 1:1829717/1‑71 (MQ=255) gTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTg < 1:3838977/71‑1 (MQ=255) tGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgc > 1:1639501/1‑71 (MQ=255) gAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCt > 1:3253266/1‑68 (MQ=255) gAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgcg > 1:2109814/1‑71 (MQ=255) aGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgcgc > 1:1996705/1‑71 (MQ=255) aGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgc > 1:652402/1‑69 (MQ=255) gCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGcc < 1:118128/71‑1 (MQ=255) cTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAAt < 1:1326799/46‑1 (MQ=255) cTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGtt < 1:2153919/54‑1 (MQ=255) cTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTg < 1:808514/55‑1 (MQ=255) cTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTg < 1:1368287/66‑1 (MQ=255) cTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCg > 1:2947416/1‑71 (MQ=255) gTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGt > 1:3526038/1‑70 (MQ=255) gTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGt > 1:3101673/1‑70 (MQ=255) tCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTg < 1:1745772/46‑1 (MQ=255) tCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGt < 1:2744230/69‑1 (MQ=255) tGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt < 1:1794706/45‑1 (MQ=255) tGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGt < 1:118692/45‑1 (MQ=255) tGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTc > 1:3244550/1‑46 (MQ=255) tGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCt < 1:545038/60‑1 (MQ=255) tGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGaaa > 1:2594989/1‑71 (MQ=255) tGAAGGACAAATAGACACGCTGCGTGACGAAGTTGCCAAATTTGt > 1:3896152/1‑45 (MQ=255) aaGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgcg < 1:3154262/61‑1 (MQ=255) aaGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAAt < 1:709335/70‑1 (MQ=255) ggACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAg > 1:2335712/1‑71 (MQ=255) gACAAATCGTCACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTgc < 1:495048/57‑1 (MQ=255) gACAAATCGACACGCTGCGTGACGAAGTTGCCAAAt < 1:3817795/36‑1 (MQ=255) cAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCAGTGAAATCAGCAt < 1:849272/71‑1 (MQ=255) aaaTCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGa < 1:245524/43‑1 (MQ=255) aaTCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGa < 1:218795/71‑1 (MQ=255) aTCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCGGCATGAg > 1:2518215/1‑71 (MQ=255) aTCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAg > 1:564638/1‑71 (MQ=255) aTCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAg > 1:2665799/1‑71 (MQ=255) tCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGc < 1:1404363/71‑1 (MQ=255) gACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCAt < 1:315798/71‑1 (MQ=255) gACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCAt > 1:3909495/1‑71 (MQ=255) acacGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCATc < 1:235430/71‑1 (MQ=255) | AAGCCATCCTGGCTGCAGCGGGTATCGCTGAAGATGTTAAGATCAGTGAGCTGTCTGAAGGACAAATCGACACGCTGCGTGACGAAGTTGCCAAATTTGTCGTTGAAGGTGATCTGCGCCGTGAAATCAGCATGAGCATC > minE/2749645‑2749784 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |