| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,754,270 | A→G | 16.4% | V413A (GTG→GCG) | trkA ← | NAD‑binding component of TrK potassium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,754,270 | 0 | A | G | 16.4% | 164.2 / 23.5 | 73 | V413A (GTG→GCG) | trkA | NAD‑binding component of TrK potassium transporter |
| Reads supporting (aligned to +/- strand): ref base A (36/25); new base G (8/4); total (44/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CATAATTACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTGCT > minE/2754200‑2754340 | cATAATTACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTacca > 1:1803417/1‑71 (MQ=255) aTTACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTaccacc > 1:1869572/1‑69 (MQ=255) aTTACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCg > 1:2100340/1‑70 (MQ=255) ttACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGc > 1:2165132/1‑70 (MQ=255) ttACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCt > 1:3147507/1‑71 (MQ=255) tACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTc > 1:2868800/1‑71 (MQ=255) aCGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCt > 1:125415/1‑69 (MQ=255) aCGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTc > 1:1858950/1‑70 (MQ=255) aCGTGATAGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTc > 1:3650954/1‑70 (MQ=255) cGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTcc < 1:3819172/70‑1 (MQ=255) gATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCaat > 1:3284685/1‑70 (MQ=255) tCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCaataat > 1:1688976/1‑71 (MQ=255) tCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCaataa > 1:2802051/1‑70 (MQ=255) tCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCaataat > 1:3430814/1‑71 (MQ=255) gCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATAACGTCGTTTCCACGTACCACCGCTCCAATAATCg < 1:2184011/71‑1 (MQ=255) ccTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGt < 1:3503663/71‑1 (MQ=255) ccTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGt < 1:3916971/71‑1 (MQ=255) cTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGtt < 1:3000130/71‑1 (MQ=255) cTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGtt < 1:3000129/71‑1 (MQ=255) gCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTc < 1:3659400/69‑1 (MQ=255) cTCAATTCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTg < 1:3398333/71‑1 (MQ=255) cTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTg < 1:1212550/71‑1 (MQ=255) cAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCAATAATCGTTCCTGGc > 1:2961885/1‑71 (MQ=255) cAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTc > 1:1426014/1‑66 (MQ=255) aaTGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCg > 1:3117864/1‑71 (MQ=255) aaTGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCg > 1:2849705/1‑71 (MQ=255) aaTGCGCAGATTGTCATTGGCAATCATCACGTCGTTTACACGTACCACCGCTc > 1:1600340/1‑53 (MQ=255) aTGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCAATAATCGTTCCTGGCgg < 1:3759239/71‑1 (MQ=255) cgcAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAg < 1:1709793/71‑1 (MQ=255) gcAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGc < 1:2221390/71‑1 (MQ=255) atgATTGCCAATGACAATCATCACGTCGTTTCCACGTACCACCGCTCCaat < 1:2948345/49‑1 (MQ=25) aGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCtt > 1:2003543/1‑71 (MQ=255) aGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCtt > 1:2853621/1‑71 (MQ=255) aTTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGa > 1:1189050/1‑71 (MQ=255) aTTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGa > 1:859621/1‑71 (MQ=255) ttGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGAt > 1:2958792/1‑71 (MQ=255) ttGTCATTGGCAATCATCACGTCGTTTCCACGTACAACCGCTCCAATAATCGTTCCTGGCGGTa > 1:3012056/1‑64 (MQ=255) ttGTCATTGGCAATCATCAAGTCGTTTCCAGGTACCGCCGCTCCAATAATCGTTACTGGc > 1:890594/1‑60 (MQ=255) tGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGAtt > 1:1826937/1‑71 (MQ=255) gTCATTGGCAATCATCACGTCGTTTCCACGTaccac > 1:1360584/1‑36 (MQ=255) gTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGAtt < 1:2406667/70‑1 (MQ=255) gTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGAtt < 1:3198318/70‑1 (MQ=255) tCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTc < 1:641584/71‑1 (MQ=255) cATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCg > 1:753087/1‑71 (MQ=255) aTTGGCAATCATCACGTCGTTTCCACGTACCGCCGCTCCAATAATCGtt > 1:3712220/1‑49 (MQ=255) aTTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCg < 1:892676/70‑1 (MQ=255) aTTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGt > 1:2983317/1‑71 (MQ=255) ggCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCa < 1:3650378/70‑1 (MQ=255) gCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAAt < 1:2507579/71‑1 (MQ=255) atcatcACGTCGTTTCCACGTACCACCGCTCCAATAATCTTTCCTGGCGGTAGCTTGATTTCGTCAATGAc > 1:2761396/1‑71 (MQ=255) tcatcaCGTCGTTTCCACGTACCGCCGCTCCAATAATCGTTc > 1:373914/1‑42 (MQ=255) atcaCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGActc < 1:2107216/70‑1 (MQ=255) tcaCGTCGTTTCCACGTACCGCCGCTCCAATAATCGt < 1:570922/37‑1 (MQ=255) tcaCGTCGTTTCCACGTACCGCCGCTCCAATAATCGt < 1:1551679/37‑1 (MQ=255) tcaCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTg > 1:3796793/1‑71 (MQ=255) tcaCGGCGGTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTg < 1:2570243/71‑1 (MQ=255) caCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCt < 1:2032883/40‑1 (MQ=255) caCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGc > 1:3702069/1‑71 (MQ=255) aCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCaa > 1:1296338/1‑61 (MQ=255) aCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGcc > 1:154556/1‑71 (MQ=255) aCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGcc < 1:250831/71‑1 (MQ=255) aCGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGcc < 1:3370326/71‑1 (MQ=255) cgtcgtTTCCACGTACCGCCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGcc > 1:2126391/1‑70 (MQ=255) gtcgtTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGa > 1:2901862/1‑71 (MQ=255) gtcgtTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGa > 1:3834140/1‑71 (MQ=255) tcgtTTCCACGTACCACCGCTCCAATAATCGTTCCTg > 1:822684/1‑37 (MQ=255) tcgtTTCCACGTACCACCGCTCCAATAATCGTTCCTg > 1:2420591/1‑37 (MQ=255) tcgtTTCCACGTACCACCGCTCCAATAATCGTTCCTg > 1:3364987/1‑37 (MQ=255) tCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAAcgc > 1:2617102/1‑71 (MQ=255) ccACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAAcgcg > 1:193923/1‑71 (MQ=255) ccACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAAcgcg > 1:1988682/1‑71 (MQ=255) cACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGt < 1:616661/71‑1 (MQ=255) cACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGt < 1:602488/71‑1 (MQ=255) gTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAg > 1:3796840/1‑71 (MQ=255) gTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAg > 1:3338160/1‑71 (MQ=255) tACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAAc < 1:2485478/63‑1 (MQ=255) accaccGCTCCAATAATCGTTCCTGGCGGTAGCTTGAtt < 1:2166471/39‑1 (MQ=255) accaccGCTCCAATAATCGTTCCTGGCGGTAGCTTGAtt < 1:3368389/39‑1 (MQ=255) ccaccGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTg < 1:2532657/71‑1 (MQ=255) ccaccGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTg > 1:3448273/1‑71 (MQ=255) caccGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTGc > 1:3439283/1‑71 (MQ=255) accGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGa < 1:809715/46‑1 (MQ=255) accGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTGCt < 1:1307833/71‑1 (MQ=255) accGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTGCt > 1:1972947/1‑71 (MQ=255) | CATAATTACGTGATCGCCTTGCTCAATGCGCAGATTGTCATTGGCAATCATCACGTCGTTTCCACGTACCACCGCTCCAATAATCGTTCCTGGCGGTAGCTTGATTTCGTCAATGACTCTGCCGACAACGCGTGAGGTGCT > minE/2754200‑2754340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |