| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,892,325 | T→C | 18.2% | intergenic (+23/‑117) | ytfQ → / → ytfR | predicted sugar transporter subunit/predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,892,325 | 0 | T | C | 18.2% | 184.8 / 35.1 | 88 | intergenic (+23/‑117) | ytfQ/ytfR | predicted sugar transporter subunit/predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (36/36); new base C (8/8); total (44/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01 | |||||||||||
TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCG > minE/2892257‑2892395 | tACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTc > 1:217898/1‑71 (MQ=255) aCCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCg > 1:2844173/1‑71 (MQ=255) ccGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCg < 1:1008660/70‑1 (MQ=255) cAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAgg > 1:1318385/1‑71 (MQ=255) cAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAgg < 1:3408682/71‑1 (MQ=255) aaaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGc < 1:1289023/71‑1 (MQ=255) aaaGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGcc < 1:3840403/71‑1 (MQ=255) aagaagAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCg < 1:950534/71‑1 (MQ=255) aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGAt < 1:2492100/67‑1 (MQ=255) aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAgg < 1:2562856/71‑1 (MQ=255) aTTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg < 1:1189055/70‑1 (MQ=255) ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc < 1:2093138/71‑1 (MQ=255) ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc > 1:3213144/1‑71 (MQ=255) ttAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgc > 1:1839881/1‑71 (MQ=255) gtggcaaatgcctgatgcgctacgcttatcaggcctacatattcttgcgccATTCGTAGGCCGGATAAGgc > 1:1537681‑M2/52‑71 (MQ=255) gtggcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGAt < 1:2557604‑M2/15‑1 (MQ=255) tgacaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGgc > 1:2898407‑M2/51‑70 (MQ=255) agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcg > 1:2436020/1‑70 (MQ=255) agagAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGgcg < 1:3308685/70‑1 (MQ=255) gcaaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAaggcgtt > 1:250992‑M2/49‑69 (MQ=255) caaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTa > 1:3572100‑M2/48‑54 (MQ=255) caaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTc < 1:1854170‑M2/24‑1 (MQ=255) caaatgcctgatgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATaaggcgtcc < 1:3576853‑M2/24‑4 (MQ=255) agaagaagaAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATa < 1:3497309/63‑1 (MQ=255) aaatgcctgatgcgctacgcttatcaggccttcagattcttgcgccATTCGTAGGCCGGATAAg > 1:1007612‑M2/47‑64 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAaggcgtt < 1:2376689‑M2/23‑3 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTtca > 1:115877‑M2/47‑70 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTtca > 1:2739671‑M2/47‑70 (MQ=255) aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcg < 1:297379/70‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCg < 1:2982146‑M2/5‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCg < 1:220638‑M2/5‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCg < 1:1858988‑M2/5‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCg > 1:3347542‑M2/43‑47 (MQ=255) tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:212568‑M2/43‑71 (MQ=255) tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:3638845‑M2/43‑71 (MQ=255) gcctgatgcgctacgcttatcaggcctacagattcttgagccATTCGTAGGCCGGATAAGGCGTTCacgc > 1:434558‑M2/43‑70 (MQ=255) aagaaAAATATGTGGTATTGAGGGTTGCTATGCCTGATGCCGAtt < 1:2897881/45‑1 (MQ=255) agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgcgc > 1:2152367/1‑69 (MQ=255) gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTa > 1:1338455/1‑47 (MQ=255) gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTa > 1:2711116/1‑47 (MQ=255) gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg < 1:3722136/58‑1 (MQ=255) gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg < 1:423714/58‑1 (MQ=255) gaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAg < 1:428465/58‑1 (MQ=255) ctgatgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCAcgccgc < 1:2768907‑M2/31‑1 (MQ=255) cctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc < 1:1742409‑M2/29‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:3424724‑M2/39‑71 (MQ=255) gatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATa < 1:3200186‑M2/16‑1 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1698281‑M2/38‑71 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1417802‑M2/38‑71 (MQ=255) atgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATc < 1:3367228‑M2/34‑1 (MQ=255) tgcgctacgcttatcaggcctacaattctcgcgccACTCGTa > 1:1545194‑M2/36‑42 (MQ=255) tgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:1593977‑M2/36‑71 (MQ=255) cgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:2959088‑M2/36‑1 (MQ=255) cgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCgg < 1:2360872‑M2/37‑1 (MQ=255) ttGGGTTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATa < 1:3541669/48‑1 (MQ=255) tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGc < 1:104092/70‑1 (MQ=255) ctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGgca < 1:1879157‑M2/39‑2 (MQ=255) tacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcat > 1:2696707‑M2/32‑69 (MQ=255) tacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag > 1:1085164‑M2/32‑69 (MQ=255) tacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt > 1:1709259‑M2/31‑71 (MQ=255) gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:47971/70‑1 (MQ=255) ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:712316/69‑1 (MQ=255) cgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag < 1:2136661‑M2/40‑3 (MQ=255) tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCg > 1:1292980/1‑39 (MQ=255) tATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCg > 1:463623/1‑39 (MQ=255) ttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:3110272‑M2/27‑58 (MQ=255) ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg > 1:2705674/1‑70 (MQ=255) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgccgc > 1:3850029/1‑55 (MQ=255) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa > 1:1298471/1‑64 (MQ=255) atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:585171‑M2/45‑1 (MQ=255) atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:339089‑M2/45‑1 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg > 1:3652576/1‑59 (MQ=18) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATcc < 1:1574127/58‑1 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGt > 1:995263/1‑67 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCa < 1:3526392/70‑1 (MQ=255) gAGGGGTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgcg < 1:1045342/50‑1 (MQ=255) aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACt < 1:1563226/71‑1 (MQ=255) gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa < 1:3731858/71‑1 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:2165178/1‑47 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:3394226/1‑47 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATg < 1:933582/60‑1 (MQ=255) acagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcaa > 1:41356‑M2/16‑53 (MQ=255) tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAGGcat > 1:1511977/1‑68 (MQ=255) aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGa > 1:3206027/1‑71 (MQ=255) tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg < 1:3604240/69‑1 (MQ=255) gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg < 1:3466736/56‑1 (MQ=255) aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg > 1:3843917/1‑71 (MQ=255) gATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc > 1:365581/1‑71 (MQ=255) aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 1:72494/1‑71 (MQ=255) ttCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg > 1:3310600/1‑71 (MQ=255) | TACCGCAAAAGAAGAATTAGAGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCG > minE/2892257‑2892395 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |