| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,892,345 | C→T | 38.7% | intergenic (+43/‑97) | ytfQ → / → ytfR | predicted sugar transporter subunit/predicted sugar transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,892,345 | 0 | C | T | 38.7% | 47.7 / 59.9 | 62 | intergenic (+43/‑97) | ytfQ/ytfR | predicted sugar transporter subunit/predicted sugar transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/19); new base T (14/10); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG > minE/2892277‑2892414 | caaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTc < 1:1854170‑M2/24‑1 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTtca > 1:2739671‑M2/47‑70 (MQ=255) aaatgcctgatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTtca > 1:115877‑M2/47‑70 (MQ=255) aagaagaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcg < 1:297379/70‑1 (MQ=255) tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:3638845‑M2/43‑71 (MQ=255) tgcctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc > 1:212568‑M2/43‑71 (MQ=255) gcctgatgcgctacgcttatcaggcctacagattcttgagccATTCGTAGGCCGGATAAGGCGTTCacgc > 1:434558‑M2/43‑70 (MQ=255) agaaAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCgcgc > 1:2152367/1‑69 (MQ=255) ctgatgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCAcgccgc < 1:2768907‑M2/31‑1 (MQ=255) cctgatgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCAcgcc < 1:1742409‑M2/29‑1 (MQ=255) gatgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCAt > 1:3424724‑M2/39‑71 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1417802‑M2/38‑71 (MQ=255) atgcgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:1698281‑M2/38‑71 (MQ=255) atgcgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATc < 1:3367228‑M2/34‑1 (MQ=255) tgcgctacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:1593977‑M2/36‑71 (MQ=255) cgctacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg < 1:2959088‑M2/36‑1 (MQ=255) cgctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCgg < 1:2360872‑M2/37‑1 (MQ=255) tGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGc < 1:104092/70‑1 (MQ=255) ctacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCGgca < 1:1879157‑M2/39‑2 (MQ=255) tacgcttatcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcat > 1:2696707‑M2/32‑69 (MQ=255) tacgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag > 1:1085164‑M2/32‑69 (MQ=255) tacgcttatcaggcctacaattctcgcgccACTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAt > 1:1709259‑M2/31‑71 (MQ=255) gggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:47971/70‑1 (MQ=255) ggTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa < 1:712316/69‑1 (MQ=255) cgcttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCATCCggcag < 1:2136661‑M2/40‑3 (MQ=255) ttatcaggcctacagattctcgcgccACTCGTAGGCCGGATAAGGCGTCCACGCCGCa > 1:3110272‑M2/27‑58 (MQ=255) ttGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg > 1:2705674/1‑70 (MQ=255) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgccgc > 1:3850029/1‑55 (MQ=255) tGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGa > 1:1298471/1‑64 (MQ=255) atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:585171‑M2/45‑1 (MQ=255) atcaggcctacagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATGGTg < 1:339089‑M2/45‑1 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATGCGGCGCGAGCGCCTTATCCg > 1:3652576/1‑59 (MQ=18) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATcc < 1:1574127/58‑1 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGt > 1:995263/1‑67 (MQ=255) gAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCa < 1:3526392/70‑1 (MQ=255) gAGGGGTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTcgcg < 1:1045342/50‑1 (MQ=255) aGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACt < 1:1563226/71‑1 (MQ=255) gTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGaa < 1:3731858/71‑1 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:2165178/1‑47 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGcgcc > 1:3394226/1‑47 (MQ=255) ttGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATg < 1:933582/60‑1 (MQ=255) acagattcttgcgccATTCGTAGGCCGGATAAGGCGTTCACGCCGCATCCggcaa > 1:41356‑M2/16‑53 (MQ=255) tATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAGGcat > 1:1511977/1‑68 (MQ=255) aTGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGa > 1:3206027/1‑71 (MQ=255) tGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTg < 1:3604240/69‑1 (MQ=255) gCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTg < 1:3466736/56‑1 (MQ=255) aTGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACg > 1:3843917/1‑71 (MQ=255) gATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAc > 1:365581/1‑71 (MQ=255) aTTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTAcc > 1:72494/1‑71 (MQ=255) ttCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcg > 1:3310600/1‑71 (MQ=255) cGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgc < 1:3038261/70‑1 (MQ=255) gTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACcgcg < 1:1273212/70‑1 (MQ=255) ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt < 1:2253388/70‑1 (MQ=255) ggCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCt > 1:3180584/1‑70 (MQ=255) gCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa < 1:275933/71‑1 (MQ=255) gCCGGATAAGGCGCACGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTa < 1:2195048/71‑1 (MQ=255) cGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTAt > 1:501586/1‑70 (MQ=255) ggATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCa < 1:124883/71‑1 (MQ=255) aTAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg < 1:2173154/71‑1 (MQ=255) aTAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATg > 1:1935292/1‑71 (MQ=255) tAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc > 1:2525184/1‑71 (MQ=255) tAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGc > 1:723037/1‑71 (MQ=255) gcgcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa < 1:430234/70‑1 (MQ=255) gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCtt < 1:450083/58‑1 (MQ=255) gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTa > 1:1043045/1‑68 (MQ=255) gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg > 1:1121590/1‑71 (MQ=255) gcTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTg > 1:1047539/1‑71 (MQ=255) | AGAAGAAGAAAAATATGGGGTATTGAGGGTTGCTATGCCTGATGCCGATTCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCGATGGTGCACTGAAGCCTGATGCGACGCTTACCGCGTCTTATCATGCCTACTG > minE/2892277‑2892414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |