| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,913,962 | G→A | 11.7% | intergenic (‑45/+236) | pyrL ← / ← yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,913,962 | 0 | G | A | 11.7% | 183.7 / 12.8 | 68 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (23/37); new base A (4/4); total (27/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATG > minE/2913892‑2914032 | aCAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAg < 1:3146564/71‑1 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:2879278/1‑71 (MQ=255) aaaaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcg > 1:694756/1‑71 (MQ=255) aaTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcggc > 1:3573197/1‑71 (MQ=255) aTGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgcggcg > 1:2982647/1‑71 (MQ=255) tGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGgcgc < 1:3664730/71‑1 (MQ=255) tCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCa < 1:3054220/70‑1 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc < 1:2514719/62‑1 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg > 1:1499593/1‑70 (MQ=255) gAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAg < 1:3151015/70‑1 (MQ=255) acacacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAt > 1:2647384/1‑71 (MQ=255) acacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:3453244/71‑1 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:1857983/71‑1 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:3525306/71‑1 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:582334/71‑1 (MQ=255) cacTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTg < 1:1602803/71‑1 (MQ=255) acTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:2072378/69‑1 (MQ=255) tGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATg > 1:3489867/1‑71 (MQ=255) aCCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCa < 1:3206468/70‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt < 1:1053919/71‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt < 1:2606511/71‑1 (MQ=255) cATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAttt > 1:1324386/1‑70 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGTCGCAAGCATTTGATGCAATAtttt < 1:1267397/70‑1 (MQ=255) aTCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:33908/53‑1 (MQ=255) tcctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGc < 1:1404568/52‑1 (MQ=255) cctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAt > 1:296942/1‑53 (MQ=255) cctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCAt > 1:1894181/1‑53 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGGAATATTTTGt > 1:3796353/1‑69 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtt < 1:1561139/65‑1 (MQ=255) ctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:2665298/71‑1 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc > 1:3252011/1‑38 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAgc > 1:2074968/1‑38 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCaa < 1:2298454/45‑1 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATAtttt > 1:3744849/1‑64 (MQ=255) ccGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAg < 1:3427694/69‑1 (MQ=255) cGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgcg > 1:3231001/1‑70 (MQ=255) ggCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAgcgc > 1:1701879/1‑70 (MQ=255) ggCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:2963944/71‑1 (MQ=255) ggCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:1479097/71‑1 (MQ=255) gCAAATTGTCCTGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:1747888/48‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:1948923/48‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaa < 1:1350167/71‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa > 1:1334736/1‑70 (MQ=255) cAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaaa < 1:124052/71‑1 (MQ=255) ttGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGt > 1:737975/1‑71 (MQ=255) tGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGtt > 1:1020872/1‑71 (MQ=255) tCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGttt < 1:2131560/70‑1 (MQ=255) tCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATA‑TTTGTCAGCGCAAACGGTTTAt < 1:2313813/71‑1 (MQ=255) ccGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa > 1:1291162/1‑61 (MQ=255) cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt < 1:3822982/70‑1 (MQ=255) ggCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAttt < 1:3472833/71‑1 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCAttt < 1:812857/36‑1 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGAt > 1:2089587/1‑39 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGAt > 1:2586340/1‑39 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg < 1:2803884/71‑1 (MQ=255) gCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTg < 1:2899928/71‑1 (MQ=255) ataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACg > 1:894234/1‑57 (MQ=255) ataCTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAt > 1:62039/1‑69 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCa < 1:2384699/45‑1 (MQ=255) cTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGAGCAAACGGTTTATTTGAATTaaa < 1:3164711/70‑1 (MQ=255) cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaa < 1:3291289/70‑1 (MQ=255) cTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaa < 1:2783022/70‑1 (MQ=255) cATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAg > 1:3894242/1‑70 (MQ=255) aTCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaaa > 1:2074535/1‑68 (MQ=255) aTCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAg > 1:3762367/1‑69 (MQ=255) aTCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:1286338/1‑70 (MQ=255) aTCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGt > 1:1524397/1‑70 (MQ=255) tCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCa < 1:211287/71‑1 (MQ=255) tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCa < 1:1563525/71‑1 (MQ=255) cGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaaa > 1:2857055/1‑66 (MQ=255) gTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAg > 1:3649359/1‑71 (MQ=255) tCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt < 1:912296/53‑1 (MQ=255) cAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAgg > 1:3101462/1‑70 (MQ=255) gAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGt > 1:3348027/1‑69 (MQ=255) aaGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc < 1:2868441/42‑1 (MQ=255) aaGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc < 1:1212490/42‑1 (MQ=255) gcggcgCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATg > 1:100914/1‑71 (MQ=255) | ACAAAATGTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGTATATG > minE/2913892‑2914032 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |