| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,918,793 | G→C | 6.4% | V301V (GTC→GTG) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,918,793 | 0 | G | C | 6.4% | 234.9 / 4.8 | 78 | V301V (GTC→GTG) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base G (41/32); new base C (3/2); total (44/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
GTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGGTCGTCA > minE/2918728‑2918859 | gTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc < 1:2289895/71‑1 (MQ=255) tATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTcc < 1:3665075/71‑1 (MQ=255) tATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc > 1:2370965/1‑70 (MQ=255) tGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCAt < 1:2537598/71‑1 (MQ=255) gCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATa > 1:2586964/1‑71 (MQ=255) cATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCAtcc > 1:3815328/1‑69 (MQ=255) tACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATAc < 1:3482601/69‑1 (MQ=255) ccGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCAcct > 1:456762/2‑60 (MQ=255) ccGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGc > 1:1610999/2‑71 (MQ=255) aCGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGc > 1:3433599/1‑71 (MQ=255) cGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACCTCCATCCCGcc < 1:2703955/71‑1 (MQ=255) cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATa > 1:483246/1‑66 (MQ=255) cGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGcc < 1:985325/71‑1 (MQ=255) ggTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCa > 1:1931823/1‑71 (MQ=255) gTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCAt < 1:2494147/71‑1 (MQ=255) ttttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATg < 1:3403002/71‑1 (MQ=255) ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATg > 1:883096/1‑69 (MQ=255) ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt < 1:3720328/70‑1 (MQ=255) ttCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGt < 1:3396146/70‑1 (MQ=255) tCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTa < 1:3683894/70‑1 (MQ=255) cGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc < 1:860264/54‑1 (MQ=255) cGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTc < 1:2049051/54‑1 (MQ=255) ccTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGc < 1:3160204/69‑1 (MQ=255) ccTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa < 1:3643457/71‑1 (MQ=255) ccTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa < 1:3791017/71‑1 (MQ=255) tGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACtt < 1:3791737/49‑1 (MQ=255) aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCaaa < 1:3074562/69‑1 (MQ=255) aTCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAtt > 1:3867180/1‑71 (MQ=255) tCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCAt > 1:190238/1‑58 (MQ=255) cAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAgg > 1:631627/1‑62 (MQ=255) cAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGTCCAAAtt > 1:1350505/1‑69 (MQ=255) cAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa < 1:2587262/65‑1 (MQ=255) cAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAttct > 1:1741476/1‑71 (MQ=255) aaaaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTa > 1:1667542/1‑59 (MQ=255) aaaaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAttctt < 1:101615/71‑1 (MQ=255) aaaaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAttctt > 1:196137/1‑71 (MQ=255) aaaaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAttct < 1:1540500/70‑1 (MQ=255) aaaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAtt > 1:1944187/1‑67 (MQ=255) aaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTcc > 1:1925411/1‑71 (MQ=255) aaaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTcc > 1:74187/1‑71 (MQ=255) aaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCaa < 1:1472181/62‑1 (MQ=255) aaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTcc > 1:1214096/1‑70 (MQ=255) aaCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCg < 1:2405858/71‑1 (MQ=255) aCAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAgg > 1:487055/1‑57 (MQ=255) cAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAgg > 1:746293/1‑56 (MQ=255) aTGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCAt < 1:288285/71‑1 (MQ=255) tGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCAtt < 1:2109237/71‑1 (MQ=255) tGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTc > 1:2847188/1‑71 (MQ=255) gcggcAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTc < 1:3884315/69‑1 (MQ=255) gcggcAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTc < 1:2541658/69‑1 (MQ=255) gcggcAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCtt < 1:1210628/71‑1 (MQ=255) cggcAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTg > 1:3817298/1‑71 (MQ=255) cggcAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTg > 1:756714/1‑71 (MQ=255) cggcAGATTCGAAGACCTCATAAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCtt > 1:2976809/1‑70 (MQ=255) cAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTa > 1:1807754/1‑42 (MQ=255) cAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGcc > 1:3310994/1‑70 (MQ=255) aGATTCGAAGACCTCATCAGTGACTTCCATACCGCCAt > 1:1284664/1‑38 (MQ=255) aTTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCg < 1:964462/54‑1 (MQ=255) aTTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCg < 1:2086230/54‑1 (MQ=255) ttCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa < 1:1876398/43‑1 (MQ=255) ttCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCa < 1:417495/43‑1 (MQ=255) tCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCa > 1:2414540/1‑66 (MQ=255) tCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGcgt > 1:2419196/1‑71 (MQ=255) tCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGcgt > 1:98349/1‑71 (MQ=255) cGAAGAACTCATCAGTGACTTCCATACCGCCATGTAGGCCaaa > 1:2632021/1‑43 (MQ=255) gAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAttcttc < 1:3394476/48‑1 (MQ=255) aaGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGcgtc < 1:464896/69‑1 (MQ=255) aGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCa > 1:807925/1‑51 (MQ=255) aGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGtt > 1:2142419/1‑71 (MQ=255) gACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAAATCTTCCGCCATTTTCTTGCCAAGCGTCGtt > 1:1492204/1‑70 (MQ=255) ccTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTgg > 1:981076/1‑71 (MQ=255) catcaGTGACTTCCATACCGCCATGTAGGCCAAATTCTTcc > 1:1633500/1‑41 (MQ=255) catcaGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGgtcg > 1:1415679/1‑71 (MQ=255) catcaGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGgtcg > 1:2231690/1‑71 (MQ=255) catcaGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGgtcg > 1:1917534/1‑71 (MQ=255) caGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAg > 1:1645646/1‑55 (MQ=255) caGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGgtc > 1:1883977/1‑67 (MQ=255) caGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGGTCGTCa > 1:2177474/1‑71 (MQ=255) gACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGtt < 1:2528289/58‑1 (MQ=255) | GTATGCATACGGTTTTCCGCCTGATCAAAAACAATGCTGGCGGCAGATTCGAAGACCTCATCAGTGACTTCCATACCGCCATGTAGGCCAAATTCTTCCGCCATTTTCTTGCCAAGCGTCGTTTGGTCGTCA > minE/2918728‑2918859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |