| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,918,948 | A→G | 10.0% | L250L (TTG→CTG) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,918,948 | 0 | A | G | 10.0% | 215.3 / 12.7 | 80 | L250L (TTG→CTG) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (49/23); new base G (6/2); total (55/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
TGGAGGAATTTGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGT > minE/2918878‑2919016 | tGGAGGAATTTGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAgcaa < 1:3708049/71‑1 (MQ=255) aTTTGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATc > 1:3179502/1‑71 (MQ=255) aTTTGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATc > 1:3514748/1‑71 (MQ=255) ttGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAgcaatgca < 1:3648205/66‑1 (MQ=255) tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:3360598/1‑71 (MQ=255) tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:1108657/1‑71 (MQ=255) tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:2072884/1‑71 (MQ=255) tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:912172/1‑71 (MQ=255) tCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGc > 1:258432/1‑71 (MQ=255) ccGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCg > 1:1385254/1‑64 (MQ=255) ccGGGTTACCGGTCAACTGCATCATCTTGCTGTTAACCTGATATTCACGCAGCAATGCAATCCg > 1:1104669/1‑64 (MQ=255) cGGGTTGTCGGTCAGCGCCATCATCTGCGCGTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGcc > 1:2463442/1‑70 (MQ=255) cGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGcc > 1:863550/1‑70 (MQ=255) gggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATACGTTCCGCCCa > 1:76264/1‑71 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt > 1:1358545/1‑71 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt < 1:795610/71‑1 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt < 1:3033334/71‑1 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt > 1:420712/1‑71 (MQ=255) tcGGTCAGCGCCATCATCTGCGCGTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTtctc > 1:1548306/2‑71 (MQ=255) ccGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACTCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:626537/71‑1 (MQ=255) cGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTctct < 1:2769450/71‑1 (MQ=255) cGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTctct < 1:1661795/71‑1 (MQ=255) ggTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtt < 1:1544956/71‑1 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:51271/1‑71 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:2494190/1‑71 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:3363136/1‑71 (MQ=255) tCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATc < 1:3909439/50‑1 (MQ=255) tCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtttt < 1:1027263/71‑1 (MQ=255) cAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATATCTCTTTTg > 1:1730296/1‑71 (MQ=255) aaCTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCa > 1:2682491/1‑59 (MQ=255) aaCTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGc > 1:3257486/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt > 1:1840161/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt > 1:605847/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCt > 1:1044817/1‑70 (MQ=255) gCATCATCTTGCTGTTCACCTGATATTCACGCAgcaatg > 1:3929065/1‑39 (MQ=255) catcatCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTccc > 1:1025794/1‑71 (MQ=255) atcatcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTcccc < 1:432329/71‑1 (MQ=255) tcatcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCt > 1:2606958/1‑65 (MQ=255) catcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCa > 1:2989203/1‑71 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:3612093/56‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:3684012/1‑59 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCa < 1:82158/70‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt > 1:2185739/1‑71 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt < 1:170022/71‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt < 1:1746360/71‑1 (MQ=255) ttGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:193804/1‑71 (MQ=255) tGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:2493869/1‑70 (MQ=255) gCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt < 1:2411593/59‑1 (MQ=255) cgcgTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGaca < 1:1200300/68‑1 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg < 1:2756031/52‑1 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCca > 1:2331851/1‑71 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCAGTTCCGCCCATTTCTCTTTTGCTTACCCCATCGACAcc > 1:3538357/1‑69 (MQ=255) tCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCca > 1:481678/1‑70 (MQ=255) tCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCca > 1:2568887/1‑70 (MQ=255) gaCCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg > 1:670768/2‑50 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCag > 1:3255679/1‑39 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtttt > 1:1933862/1‑49 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:2185709/1‑48 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg > 1:1670275/1‑50 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGac > 1:1427615/1‑64 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcaca > 1:3413846/1‑71 (MQ=255) cACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcaca > 1:1024526/1‑71 (MQ=255) aCCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcaca < 1:3949957/70‑1 (MQ=255) ccTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcacaca < 1:969493/71‑1 (MQ=255) cTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACAt > 1:1749662/1‑71 (MQ=255) cTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcacacgt > 1:2889099/1‑69 (MQ=255) gATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCg > 1:1249276/1‑71 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:3349437/39‑1 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATc < 1:355764/69‑1 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATc < 1:1851072/69‑1 (MQ=255) tatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGaca > 1:3509406/1‑58 (MQ=255) tatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg < 1:50180/70‑1 (MQ=255) atTCACGCAGCAATGCAATCCGTTACGCCCATTTCTCttt > 1:3344323/1‑40 (MQ=255) ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta < 1:1807509/70‑1 (MQ=255) ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta < 1:1984513/70‑1 (MQ=255) tCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:3447658/1‑53 (MQ=255) ccgcGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGtata < 1:1419790/68‑1 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATc > 1:595954/1‑50 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta > 1:3654838/1‑68 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta > 1:2973078/1‑68 (MQ=255) aCGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTcc > 1:2217115/1‑43 (MQ=255) cGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGCCACCCACACATCGGTATAGa > 1:2953382/1‑70 (MQ=255) gcagcaGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGAt > 1:651492/1‑70 (MQ=255) gcaatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAg < 1:819556/71‑1 (MQ=255) caatgcaatCCGTTCCGCCAATTTCTCTTTTGCTTCCCCCATCGACACCcacaca > 1:1198134/1‑55 (MQ=255) aatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt > 1:302257/1‑70 (MQ=255) aatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt < 1:3871636/70‑1 (MQ=255) | TGGAGGAATTTGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGT > minE/2918878‑2919016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |