| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,918,958 | T→C | 13.5% | E246E (GAA→GAG) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,918,958 | 0 | T | C | 13.5% | 238.7 / 26.5 | 96 | E246E (GAA→GAG) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (52/31); new base C (11/2); total (63/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.71e-01 | |||||||||||
TGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTC > minE/2918888‑2919026 | tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:912172/1‑71 (MQ=255)tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:2072884/1‑71 (MQ=255)tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:3360598/1‑71 (MQ=255)tGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGt > 1:1108657/1‑71 (MQ=255) tCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGc > 1:258432/1‑71 (MQ=255) cGGGTTGTCGGTCAGCGCCATCATCTGCGCGTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGcc > 1:2463442/1‑70 (MQ=255) cGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGcc > 1:863550/1‑70 (MQ=255) gggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATACGTTCCGCCCa > 1:76264/1‑71 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt < 1:795610/71‑1 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt > 1:420712/1‑71 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt > 1:1358545/1‑71 (MQ=255) ggTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCAt < 1:3033334/71‑1 (MQ=255) tcGGTCAGCGCCATCATCTGCGCGTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTtctc > 1:1548306/2‑71 (MQ=255) ccGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACTCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:626537/71‑1 (MQ=255) cGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTctct < 1:2769450/71‑1 (MQ=255) cGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTctct < 1:1661795/71‑1 (MQ=255) ggTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtt < 1:1544956/71‑1 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:2494190/1‑71 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:3363136/1‑71 (MQ=255) gTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:51271/1‑71 (MQ=255) tCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtttt < 1:1027263/71‑1 (MQ=255) cAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATATCTCTTTTg > 1:1730296/1‑71 (MQ=255) aaCTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCa > 1:2682491/1‑59 (MQ=255) aaCTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGc > 1:3257486/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt > 1:1840161/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt > 1:605847/1‑71 (MQ=255) cTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCt > 1:1044817/1‑70 (MQ=255) catcatCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTccc > 1:1025794/1‑71 (MQ=255) atcatcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTcccc < 1:432329/71‑1 (MQ=255) tcatcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCt > 1:2606958/1‑65 (MQ=255) catcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCa > 1:2989203/1‑71 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:3612093/56‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:3684012/1‑59 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCa < 1:82158/70‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt < 1:170022/71‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt < 1:1746360/71‑1 (MQ=255) atcTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCAt > 1:2185739/1‑71 (MQ=255) ttGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:193804/1‑71 (MQ=255) tGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:2493869/1‑70 (MQ=255) gCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCtt < 1:2411593/59‑1 (MQ=255) cgcgTTCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGaca < 1:1200300/68‑1 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg < 1:2756031/52‑1 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCca > 1:2331851/1‑71 (MQ=255) ttCACCTGATATTCACGCAGCAATGCAATCAGTTCCGCCCATTTCTCTTTTGCTTACCCCATCGACAcc > 1:3538357/1‑69 (MQ=255) tCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCca > 1:481678/1‑70 (MQ=255) tCACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCca > 1:2568887/1‑70 (MQ=255) gaCCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg > 1:670768/2‑50 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCag > 1:3255679/1‑39 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCtttt > 1:1933862/1‑49 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCttt > 1:2185709/1‑48 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTg > 1:1670275/1‑50 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGac > 1:1427615/1‑64 (MQ=255) cACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcaca > 1:3413846/1‑71 (MQ=255) cACCTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcaca > 1:1024526/1‑71 (MQ=255) aCCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcaca < 1:3949957/70‑1 (MQ=255) ccTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCcacaca < 1:969493/71‑1 (MQ=255) cTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACAt > 1:1749662/1‑71 (MQ=255) cTGATACCCGCGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcacacgt > 1:2889099/1‑69 (MQ=255) gATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCg > 1:1249276/1‑71 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTtctc < 1:3349437/39‑1 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATc < 1:1851072/69‑1 (MQ=255) atatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATc < 1:355764/69‑1 (MQ=255) tatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGaca > 1:3509406/1‑58 (MQ=255) tatTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg < 1:50180/70‑1 (MQ=255) atTCACGCAGCAATGCAATCCGTTACGCCCATTTCTCttt > 1:3344323/1‑40 (MQ=255) ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta < 1:1984513/70‑1 (MQ=255) ttCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta < 1:1807509/70‑1 (MQ=255) tCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGa > 1:3447658/1‑53 (MQ=255) ccgcGCAGCAGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGtata < 1:1419790/68‑1 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATc > 1:595954/1‑50 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta > 1:3654838/1‑68 (MQ=255) cACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGta > 1:2973078/1‑68 (MQ=255) aCGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTcc > 1:2217115/1‑43 (MQ=255) cGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGCCACCCACACATCGGTATAGa > 1:2953382/1‑70 (MQ=255) gcagcaGCGCAATCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGAt > 1:651492/1‑70 (MQ=255) gcaatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAg < 1:819556/71‑1 (MQ=255) caatgcaatCCGTTCCGCCAATTTCTCTTTTGCTTCCCCCATCGACACCcacaca > 1:1198134/1‑55 (MQ=255) aatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt < 1:3871636/70‑1 (MQ=255) aatgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt > 1:302257/1‑70 (MQ=255) tgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt < 1:3139908/68‑1 (MQ=255) tgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGt < 1:1206775/68‑1 (MQ=255) tgcaatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTc > 1:1119927/1‑69 (MQ=255) gcaatCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcaca > 1:314329/1‑49 (MQ=19) gcaatCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcaca > 1:3708127/1‑49 (MQ=19) gcaatCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCcaca > 1:2594350/1‑49 (MQ=19) caatCCGTTCCGCCCATTTCTCTTTTGCTTccccc < 1:1752156/35‑1 (MQ=255) caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg > 1:3543184/1‑54 (MQ=255) caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCgg > 1:1299522/1‑54 (MQ=255) caatCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCa > 1:893447/1‑71 (MQ=255) atCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAc > 1:2022788/1‑70 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCg > 1:71625/1‑50 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAcc > 1:3408915/1‑70 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCAcc > 1:1399315/1‑70 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCt < 1:1084522/71‑1 (MQ=255) tCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCt < 1:3320291/71‑1 (MQ=255) tCCGCTCTGCCCACTTCTCTTTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGccc > 1:2377632/1‑70 (MQ=255) tCCGCTCTGCCCACTTCTCTCTGGCCTCGCCCATCGACACCCACACGTCGGTATAGATAAAGTCCGCGccc > 1:2449673/1‑70 (MQ=255) cGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCca > 1:954254/1‑41 (MQ=255) cGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTc < 1:657793/71‑1 (MQ=255) cGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTc < 1:1222338/71‑1 (MQ=255) | TGACCTCCGGGTTACCGGTCAACTGCATCATCTTGCTGTTCACCTGATATTCACGCAGCAATGCAATCCGTTCCGCCCATTTCTCTTTTGCTTCCCCCATCGACACCCACACATCGGTATAGATAAAGTCAGCACCTTC > minE/2918888‑2919026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |