| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,832 | C→G | 7.4% | A347A (GCG→GCC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,832 | 0 | C | G | 7.4% | 242.3 / 5.4 | 80 | A347A (GCG→GCC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (29/45); new base G (4/2); total (33/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTA > minE/311762‑311900 | cAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc > 1:2326278/1‑71 (MQ=255) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATc < 1:1798241/70‑1 (MQ=255) aaTGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCg < 1:1150541/71‑1 (MQ=255) tGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCt > 1:207882/1‑71 (MQ=255) ggTGGGGATCAGCGTTGCTCGCATATTCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCtt > 1:3127576/1‑71 (MQ=2) tCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCg < 1:2622455/71‑1 (MQ=255) aaCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:3276463/70‑1 (MQ=255) aCGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtt < 1:884341/71‑1 (MQ=255) cGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGttt < 1:3800745/71‑1 (MQ=255) tCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:2852763/71‑1 (MQ=255) gcgGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCg < 1:1585492/62‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:568395/64‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt > 1:2785062/1‑71 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACtt < 1:1908208/71‑1 (MQ=255) gAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACt > 1:243692/1‑70 (MQ=255) atattCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTc > 1:688874/4‑71 (MQ=16) aaGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc < 1:2326430/71‑1 (MQ=255) aGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCg < 1:576963/71‑1 (MQ=255) gTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGt < 1:3177601/71‑1 (MQ=255) ttCTGCAAGAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTg < 1:2640254/71‑1 (MQ=21) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGc > 1:3766991/1‑42 (MQ=255) cTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:2822574/1‑60 (MQ=255) gCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:2295321/56‑1 (MQ=255) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa < 1:2749706/70‑1 (MQ=255) cAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCCTGAATa < 1:912443/70‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGa > 1:23756/1‑43 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATag < 1:998935/70‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATag < 1:41066/70‑1 (MQ=255) aGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATag < 1:1467086/70‑1 (MQ=255) ggAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa > 1:3036528/1‑68 (MQ=255) gAACAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGtt < 1:1705182/49‑1 (MQ=16) gAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATa > 1:3152570/1‑67 (MQ=255) aaCATATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATagaaag > 1:936893/1‑68 (MQ=255) aaCAGATACATCACCAGGAACACCAGCATAATGGCTTCGAACAGCGTTTTTACCACTTCGTGAATagaaag > 1:2180403/1‑68 (MQ=18) aCAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTa < 1:665536/50‑1 (MQ=255) cAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAttt < 1:2916420/71‑1 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt > 1:2720296/1‑71 (MQ=255) aGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:2519929/71‑1 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGAtttt < 1:2376201/70‑1 (MQ=255) gATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTc < 1:16216/71‑1 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTc > 1:1030851/1‑54 (MQ=255) aTACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:2419244/1‑71 (MQ=255) tACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGtt > 1:1949651/1‑42 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc < 1:1544294/45‑1 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg > 1:3619781/1‑70 (MQ=255) cATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg > 1:3199439/1‑70 (MQ=255) aTAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGaa < 1:458249/71‑1 (MQ=255) tAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACg < 1:1422026/68‑1 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg > 1:3267093/1‑71 (MQ=255) aaCCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACg > 1:3135286/1‑71 (MQ=255) ccAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGc < 1:1785394/71‑1 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACggag < 1:1311492/71‑3 (MQ=255) cAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCg > 1:1722786/1‑71 (MQ=255) aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:1272663/71‑1 (MQ=255) aGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgt < 1:553765/71‑1 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAAcc < 1:2593737/38‑1 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAAc > 1:1449251/1‑37 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:1442507/71‑1 (MQ=255) ggAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtg < 1:1604772/71‑1 (MQ=255) aaCACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt < 1:2208850/71‑1 (MQ=255) cacGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCgtggt < 1:2805917/69‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACgg > 1:392723/1‑61 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg < 1:1542576/71‑1 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg > 1:1150993/1‑71 (MQ=255) cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg > 1:1162280/1‑71 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGt < 1:2697793/71‑1 (MQ=255) gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGt < 1:1905565/71‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:2057586/71‑1 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:2392995/1‑71 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa > 1:1894549/1‑71 (MQ=255) aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa < 1:2483885/71‑1 (MQ=255) ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCa > 1:2842723/1‑51 (MQ=255) ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt < 1:3598567/71‑1 (MQ=255) ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:609365/1‑71 (MQ=255) ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1509933/1‑71 (MQ=255) ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTAt > 1:3645292/1‑71 (MQ=255) gatgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATg < 1:3214708/71‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt < 1:1875796/70‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:2937811/71‑1 (MQ=255) tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATagag > 1:158440/1‑38 (MQ=255) tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATagag > 1:795887/1‑38 (MQ=255) tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:85513/70‑1 (MQ=255) | CAATGGTCGGAATCAACGTCGCGCGGAAGTTCTGCAGGAACAGATACATAACCAGGAACACGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTA > minE/311762‑311900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |