Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,220	G→T	12.3%	R171R (CGG→CGT)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,220	0	G	T	12.3%	250.2 / 13.5	81	R171R (CGG→CGT)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base G (43/28);  new base T (6/4);  total (49/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTG  >  minE/132151‑132289                                                                      |                                                                      cTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGa                                                                      >  1:2375227/1‑71 (MQ=255)     aaCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                  >  1:1834634/1‑71 (MQ=255)      aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                  <  1:3546386/70‑1 (MQ=255)       cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                >  1:2340633/1‑71 (MQ=255)        aGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGAt                                                               >  1:2146896/1‑71 (MQ=255)          tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                             <  1:2289256/71‑1 (MQ=255)          tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                             <  1:1865246/71‑1 (MQ=255)            cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                   >  1:2128209/1‑63 (MQ=255)            cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                           <  1:1654283/71‑1 (MQ=255)            cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                           >  1:1154686/1‑71 (MQ=255)            cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                            >  1:2360845/1‑70 (MQ=255)               tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                          >  1:3672365/1‑69 (MQ=255)                cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                          <  1:1911822/68‑1 (MQ=255)                  cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                     >  1:1559944/1‑71 (MQ=255)                   gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                    >  1:3282126/1‑71 (MQ=255)                     tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                        >  1:2689060/1‑65 (MQ=255)                     tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                  >  1:1378704/1‑71 (MQ=255)                     tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                  >  1:3699296/1‑71 (MQ=255)                          gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                              <  1:2961241/70‑1 (MQ=255)                           atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                >  1:597024/1‑51 (MQ=255)                           atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                >  1:788052/1‑51 (MQ=255)                             atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                          >  1:312576/1‑71 (MQ=255)                             atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                          >  1:524861/1‑71 (MQ=255)                             atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                              <  1:232113/67‑1 (MQ=255)                              tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                          >  1:3279009/1‑70 (MQ=255)                              tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                          >  1:1589625/1‑70 (MQ=255)                               cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                          <  1:820672/53‑1 (MQ=255)                                aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                       <  1:3378901/71‑1 (MQ=255)                                aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                                                      >  1:2996407/1‑56 (MQ=255)                                aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                       <  1:1866093/71‑1 (MQ=255)                                aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                       <  1:3916233/71‑1 (MQ=255)                                 ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                             >  1:501805/1‑64 (MQ=255)                                 ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                       <  1:895428/70‑1 (MQ=255)                                 ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                       >  1:369444/1‑70 (MQ=255)                                 ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                      >  1:3018776/1‑71 (MQ=255)                                  cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                     >  1:3459369/1‑71 (MQ=255)                                  cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                     >  1:1094608/1‑71 (MQ=255)                                  aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                     >  1:2295944/2‑71 (MQ=255)                                   aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                   >  1:3632017/1‑40 (MQ=255)                                   aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                   >  1:3328833/1‑40 (MQ=255)                                   aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                     >  1:1704122/1‑70 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                            >  1:2133987/1‑46 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                          <  1:3474398/48‑1 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                        >  1:413424/1‑66 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                          <  1:3129806/64‑1 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                    >  1:2795204/1‑70 (MQ=255)                                    tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                   <  1:822055/71‑1 (MQ=255)                                     cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                   <  1:353302/67‑1 (MQ=255)                                     cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                        >  1:1445872/1‑65 (MQ=255)                                      acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                        >  1:1816522/1‑48 (MQ=255)                                      acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                   >  1:938210/1‑69 (MQ=255)                                      acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                                          >  1:538351/1‑46 (MQ=255)                                       cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                  <  1:1085283/69‑1 (MQ=255)                                        acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                   <  1:2242153/51‑1 (MQ=255)                                        acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                               >  1:3892751/1‑71 (MQ=255)                                        acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                               >  1:1215807/1‑71 (MQ=255)                                          cGGATGACGAAATCATGCAACATCGGCGGATTGCg                                                                 >  1:192327/1‑35 (MQ=255)                                           ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                            <  1:1739920/71‑1 (MQ=255)                                              tGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                            <  1:1927416/36‑1 (MQ=255)                                              tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                               >  1:3322513/1‑65 (MQ=255)                                               gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                              <  1:2582517/49‑1 (MQ=255)                                               gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                              <  1:368264/49‑1 (MQ=255)                                                 cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                   <  1:3826494/58‑1 (MQ=255)                                                 cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                   <  1:2036055/58‑1 (MQ=255)                                                  gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                       <  1:2685332/66‑1 (MQ=255)                                                    aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                           <  1:3338305/47‑1 (MQ=255)                                                    aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                   >  1:74025/1‑71 (MQ=255)                                                     aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                             <  1:3299476/44‑1 (MQ=255)                                                     aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                           >  1:2171812/1‑46 (MQ=255)                                                      tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                 <  1:3405915/71‑1 (MQ=255)                                                      tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                 >  1:477006/1‑71 (MQ=255)                                                       cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                >  1:643085/1‑71 (MQ=255)                                                         tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                            >  1:2239041/1‑41 (MQ=255)                                                          gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                      >  1:1559300/1‑62 (MQ=255)                                                          gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc             >  1:664640/1‑71 (MQ=255)                                                           cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt            <  1:1122052/71‑1 (MQ=255)                                                            aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt           <  1:1854168/71‑1 (MQ=255)                                                               aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg        >  1:1491341/1‑71 (MQ=255)                                                                tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg        >  1:507433/1‑70 (MQ=255)                                                                tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc       <  1:3760758/71‑1 (MQ=255)                                                                tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc       <  1:589586/71‑1 (MQ=255)                                                                   gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc    <  1:1494823/71‑1 (MQ=255)                                                                    cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt   >  1:1326631/1‑71 (MQ=255)                                                                     ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg  >  1:2282189/1‑71 (MQ=255)                                                                     ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg  >  1:3369448/1‑71 (MQ=255)                                                                      |                                                                      CTATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTG  >  minE/132151‑132289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑