| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,159 | A→C | 10.0% | L179L (CTT→CTG) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,159 | 0 | A | C | 10.0% | 158.8 / 14.4 | 70 | L179L (CTT→CTG) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base A (32/31); new base C (4/3); total (36/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
CCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTT > minE/2919095‑2919229 | ccGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc > 1:322476/1‑71 (MQ=255) cGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc < 1:2202825/70‑1 (MQ=255) gTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgccg > 1:2350324/1‑70 (MQ=255) aaCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgccg > 1:941793/1‑68 (MQ=255) aaCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgccg > 1:641342/1‑68 (MQ=255) aaCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAg > 1:2556612/1‑71 (MQ=255) aaCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGACGCAg > 1:3089947/1‑71 (MQ=255) aCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAg < 1:543678/70‑1 (MQ=255) aCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGc > 1:1150364/1‑71 (MQ=255) aCCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGc < 1:3053122/71‑1 (MQ=255) cAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCtt > 1:1277108/1‑71 (MQ=255) cgcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGa < 1:1326754/71‑1 (MQ=255) gcAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAg < 1:2420820/71‑1 (MQ=255) cAGCTTCCGGCCAGCACGCTTGTGGCGCGCCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAg > 1:3120473/1‑70 (MQ=255) ttCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCg > 1:923315/1‑64 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATc > 1:1532503/1‑69 (MQ=255) tCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg > 1:1002485/1‑70 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGcgcc > 1:2759945/1‑53 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:1766788/69‑1 (MQ=255) ccGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGaa > 1:3638961/1‑71 (MQ=255) cGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAAt > 1:1093903/1‑71 (MQ=255) cAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:258419/71‑1 (MQ=255) aGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGcc < 1:3182796/70‑1 (MQ=255) aCGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtg > 1:3831688/1‑71 (MQ=255) gCTTGTGGCGCGACCAGACGCAAATCCGGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:750023/71‑1 (MQ=255) gCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:2695455/71‑1 (MQ=255) cTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgtt < 1:225363/70‑1 (MQ=255) ttGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGc < 1:410862/52‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt > 1:1885405/1‑69 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttgt < 1:2005970/69‑1 (MQ=255) gtgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATgttgtt < 1:2893317/70‑1 (MQ=255) tgGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAc < 1:553622/71‑1 (MQ=255) gcgcGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGGATCGAATTGCCCATGTTGTTAcgc > 1:3063692/1‑71 (MQ=255) gcgcGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCg < 1:2546399/51‑1 (MQ=255) cgcgTCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAtgttg < 1:1486235/64‑1 (MQ=255) gcgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCAt < 1:2133395/59‑1 (MQ=255) gcgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcgcgc < 1:2179825/71‑1 (MQ=255) gcgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAcgcgc < 1:2175231/71‑1 (MQ=255) cgACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATgttgtt > 1:783572/1‑64 (MQ=255) aacAGGCGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATc > 1:242975/3‑71 (MQ=255) aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGt > 1:1056823/1‑70 (MQ=255) aCCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTc < 1:1261596/71‑1 (MQ=255) ccAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGa < 1:1829194/39‑1 (MQ=255) gACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCt > 1:282821/1‑71 (MQ=255) gACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCt > 1:3557828/1‑71 (MQ=255) gcGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGccc > 1:359579/2‑69 (MQ=255) ccGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg > 1:652588/2‑71 (MQ=255) aCGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTg > 1:281883/1‑71 (MQ=255) cGCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGccc < 1:1487388/69‑2 (MQ=255) cGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGc < 1:1517398/71‑1 (MQ=255) gCAGATCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGcg > 1:2437121/1‑70 (MQ=255) cAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCAt < 1:2048631/71‑1 (MQ=255) agaTCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTa < 1:1072696/69‑1 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCa > 1:2616502/1‑69 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:1711077/71‑1 (MQ=255) aaaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATa < 1:3293749/71‑1 (MQ=255) aaTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAg < 1:3413603/71‑1 (MQ=255) aTCCAGCCCGGTCAGCGCCGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTgcgcgca < 1:3216249/58‑2 (MQ=25) aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCGATGTTGTTACGCGCGTCACCTGCATAg < 1:1724833/70‑1 (MQ=255) aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGa > 1:992121/1‑71 (MQ=255) aTCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGa > 1:2904147/1‑71 (MQ=255) cAGCCCGGTCAGCGCGGCCGCTTCCAGCATCGAGTTGCCCATGTTGTTGCGCGCATCGCCCGCGTAGAc > 1:2948535/1‑69 (MQ=255) cAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCa > 1:1661717/1‑58 (MQ=255) gACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGc > 1:1582406/1‑71 (MQ=255) acaggTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCg > 1:18140/4‑71 (MQ=255) aCCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCa < 1:383810/68‑1 (MQ=255) ccGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:3004126/1‑71 (MQ=255) ccGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt < 1:205712/71‑1 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTAc < 1:1661539/45‑1 (MQ=255) cGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGt > 1:3549354/1‑70 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGAc > 1:2726544/1‑60 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTc > 1:3090568/1‑67 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAttt > 1:721565/1‑71 (MQ=255) aaGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCAtt > 1:3856891/1‑70 (MQ=255) | CCGTAACCAGCGCAGCTTCCGGCCAGCACGCTTGTGGCGCGACCAGACGCAAATCCAGACCGGTAAGCGCCGCAGCTTCGAGCATCGAATTGCCCATGTTGTTACGCGCGTCACCTGCATAGACCAGCGTCATTT > minE/2919095‑2919229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |