| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,465 | G→T | 14.5% | L77L (CTC→CTA) ‡ | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,465 | 0 | G | T | 14.5% | 278.7 / 22.4 | 96 | L77L (CTC→CTA) ‡ | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base G (43/39); new base T (8/6); total (51/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
Only 100 of 102 total aligned reads displayed.
GCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT > minE/2919399‑2919534 | gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga > 1:1671216/1‑70 (MQ=255)gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga > 1:2474233/1‑70 (MQ=255)gCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgaga > 1:286668/1‑70 (MQ=255) cGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGAt > 1:628717/1‑70 (MQ=255) gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa < 1:3385590/71‑1 (MQ=255) gACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATaa < 1:1545066/71‑1 (MQ=255) ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg > 1:2606645/1‑70 (MQ=255) ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg > 1:3253438/1‑70 (MQ=255) ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg > 1:3744153/1‑70 (MQ=255) ccAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAg > 1:1435759/1‑70 (MQ=255) cAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTa < 1:1338284/71‑1 (MQ=255) cAAGCACGCGGGCAGTGTCTTTAATAGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt > 1:3808790/1‑70 (MQ=255) aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc > 1:2019871/1‑71 (MQ=255) aGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc < 1:800302/71‑1 (MQ=255) cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc < 1:198903/71‑1 (MQ=255) cACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcg > 1:1024561/1‑70 (MQ=255) cgcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa < 1:1212140/71‑1 (MQ=255) gcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg > 1:3674753/1‑71 (MQ=255) gcgGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg > 1:2676850/1‑71 (MQ=255) cAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCAccc > 1:1171111/1‑71 (MQ=255) gtgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGccc < 1:1796656/69‑1 (MQ=255) tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa > 1:2262576/1‑57 (MQ=255) tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg > 1:3772954/1‑71 (MQ=255) tgtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg > 1:305225/1‑71 (MQ=255) tgtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAc > 1:1536508/1‑58 (MQ=255) gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTgg < 1:1451349/70‑1 (MQ=255) gtCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGt > 1:2564401/1‑71 (MQ=255) gtCCTTAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAAcgc < 1:1223077/59‑1 (MQ=255) tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCga > 1:1111400/1‑46 (MQ=255) tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa > 1:135462/1‑60 (MQ=255) tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa > 1:3227485/1‑60 (MQ=255) tCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa > 1:140120/1‑60 (MQ=255) tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGt < 1:3029960/51‑1 (MQ=255) tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc < 1:2679631/56‑1 (MQ=255) tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAg > 1:456162/1‑59 (MQ=255) tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa > 1:3480390/1‑70 (MQ=255) tttAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCa > 1:910340/1‑70 (MQ=255) tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat > 1:3334071/1‑71 (MQ=255) tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat > 1:1032572/1‑71 (MQ=255) tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAAGCGAGCACCCTGGTCatat > 1:3624586/1‑71 (MQ=255) aaTTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCt > 1:2810014/1‑62 (MQ=255) aTCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAc < 1:3024510/50‑1 (MQ=255) tCGACTCTTTATGACCAATCTGGCTGACGCTTGGGCCGAGATAAGTAACGCGAGCACCCt > 1:1648351/1‑60 (MQ=255) gACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCgag < 1:1462822/38‑1 (MQ=255) aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGAt < 1:889712/39‑1 (MQ=255) aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa < 1:1961293/45‑1 (MQ=255) aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa < 1:1679702/71‑1 (MQ=255) ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa > 1:3308221/1‑71 (MQ=255) tctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGc > 1:1662501/1‑68 (MQ=255) tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa > 1:2704415/1‑70 (MQ=255) tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc > 1:788008/1‑71 (MQ=255) tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc < 1:962910/69‑1 (MQ=255) tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCATGGTCATATGCGGCAAc < 1:1954740/69‑1 (MQ=255) ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc < 1:2668362/65‑1 (MQ=255) tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc < 1:339524/70‑1 (MQ=255) tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:575732/1‑70 (MQ=255) aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTaa > 1:2501674/1‑38 (MQ=255) aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg < 1:417330/70‑1 (MQ=255) aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg > 1:3808433/1‑70 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:1746299/71‑1 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:773765/70‑1 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:3506041/70‑1 (MQ=255) gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc < 1:503415/58‑1 (MQ=255) gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGAACCCTTGACATATGc < 1:1341441/58‑1 (MQ=255) cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa < 1:3064838/69‑1 (MQ=255) cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAag < 1:3312819/70‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc < 1:1027469/38‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGa > 1:965550/1‑40 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa < 1:244577/70‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag < 1:1603931/71‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag < 1:2414506/71‑1 (MQ=255) ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa > 1:3400372/1‑69 (MQ=255) ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa > 1:1550738/1‑69 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAcgc > 1:657330/1‑36 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCCCCCTGGTCATATGCGGCAACTTCGAAagag > 1:2090553/1‑71 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAagag > 1:2074159/1‑71 (MQ=255) aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa < 1:3100397/71‑1 (MQ=255) tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt > 1:1266996/1‑71 (MQ=255) cTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCa > 1:2226734/1‑37 (MQ=255) tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc > 1:2709100/1‑50 (MQ=255) tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg > 1:1136939/1‑71 (MQ=255) cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGt < 1:1150435/71‑1 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc > 1:1021955/1‑70 (MQ=255) ccGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc > 1:1352634/1‑70 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACGCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1755589/69‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:47219/71‑1 (MQ=255) cGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga > 1:1256399/1‑58 (MQ=255) gCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag < 1:3292804/71‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATg < 1:2390389/40‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:3930069/61‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt > 1:175560/1‑71 (MQ=255) tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc > 1:764412/1‑70 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg < 1:2916072/70‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:733628/69‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:3227791/69‑1 (MQ=255) ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag < 1:509753/71‑1 (MQ=255) ccGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACtt > 1:3289058/1‑43 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:519303/46‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt > 1:676457/1‑71 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGt > 1:3365593/1‑57 (MQ=255) | GCGACCAAGCACGCGGGCAGTGTCTTTAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCT > minE/2919399‑2919534 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |