| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,493 | T→A | 22.0% | Y68F (TAT→TTT) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,493 | 0 | T | A | 22.0% | 187.8 / 51.1 | 91 | Y68F (TAT→TTT) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (26/45); new base A (11/9); total (37/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC > minE/2919425‑2919562 | tAATTGACTCTTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCaaat > 1:3334071/1‑71 (MQ=255)tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCatat > 1:1032572/1‑71 (MQ=255)tAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAAAGCGAGCACCCTGGTCatat > 1:3624586/1‑71 (MQ=255) aCTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCa < 1:1679702/71‑1 (MQ=255) ctctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa > 1:3308221/1‑71 (MQ=255) tctTTATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGc > 1:1662501/1‑68 (MQ=255) tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCaa > 1:2704415/1‑70 (MQ=255) tctTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc > 1:788008/1‑71 (MQ=255) tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAAc < 1:962910/69‑1 (MQ=255) tttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCATGGTCATATGCGGCAAc < 1:1954740/69‑1 (MQ=255) ttATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGc < 1:2668362/65‑1 (MQ=255) tATGCCCAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTc < 1:339524/70‑1 (MQ=255) tATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTc > 1:575732/1‑70 (MQ=255) aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg < 1:417330/70‑1 (MQ=255) aTGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCg > 1:3808433/1‑70 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:1746299/71‑1 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:3506041/70‑1 (MQ=255) tGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGa < 1:773765/70‑1 (MQ=255) gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc < 1:503415/58‑1 (MQ=255) gACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGAACCCTTGACATATGc < 1:1341441/58‑1 (MQ=255) cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa < 1:3064838/69‑1 (MQ=255) cccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAag < 1:3312819/70‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaaa < 1:244577/70‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag < 1:1603931/71‑1 (MQ=255) aCCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAag < 1:2414506/71‑1 (MQ=255) ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa > 1:1550738/1‑69 (MQ=255) ccAATCTGGCTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGaaa > 1:3400372/1‑69 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCCCCCTGGTCATATGCGGCAACTTCGAAagag > 1:2090553/1‑71 (MQ=255) cAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAagag > 1:2074159/1‑71 (MQ=255) aTCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa < 1:3100397/71‑1 (MQ=255) tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt > 1:1266996/1‑71 (MQ=255) tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGc > 1:2709100/1‑50 (MQ=255) tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg > 1:1136939/1‑71 (MQ=255) gCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:988330/68‑1 (MQ=255) cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGt < 1:1150435/71‑1 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc > 1:1021955/1‑70 (MQ=255) ccGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc > 1:1352634/1‑70 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACGCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1755589/69‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:47219/71‑1 (MQ=255) cGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAaga > 1:1256399/1‑58 (MQ=255) gCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag < 1:3292804/71‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATg < 1:2390389/40‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:3930069/61‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt > 1:175560/1‑71 (MQ=255) tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc > 1:764412/1‑70 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg < 1:2916072/70‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:733628/69‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:3227791/69‑1 (MQ=255) ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag < 1:509753/71‑1 (MQ=255) ccGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACtt > 1:3289058/1‑43 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:2694615/46‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:519303/46‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt > 1:676457/1‑71 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGt > 1:3365593/1‑57 (MQ=255) gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt > 1:2066507/1‑71 (MQ=255) aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACt < 1:1050373/37‑1 (MQ=255) aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt < 1:1716666/71‑1 (MQ=255) aaGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCa < 1:2993297/46‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2915227/71‑1 (MQ=255) tAACGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:2984119/37‑1 (MQ=255) aaCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagtcg < 1:1874776/56‑1 (MQ=255) aCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:3790563/71‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGaa < 1:3311641/35‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc < 1:635119/65‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:902519/71‑1 (MQ=255) aCGCGAGCACCCTGGACATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:3738397/71‑1 (MQ=255) cgcgcgcgCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCGTTTTCGAAGAt > 1:1109394/1‑70 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGt < 1:278775/46‑1 (MQ=255) gcgcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:590861/69‑1 (MQ=255) gcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt > 1:1052411/1‑69 (MQ=255) gcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa < 1:360040/71‑1 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg < 1:2008898/71‑1 (MQ=255) gAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg > 1:1141367/1‑65 (MQ=255) gcgcccTGGTCAAATGCGGGAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcgc > 1:45921/4‑71 (MQ=255) gcgcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcgc > 1:3311355/4‑71 (MQ=255) cACCCTGGTCATATGCTGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg < 1:2405417/71‑1 (MQ=255) cACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:764474/42‑1 (MQ=255) cACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgc < 1:2483171/68‑1 (MQ=255) gcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa < 1:2332387/70‑1 (MQ=255) gcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa < 1:3713242/70‑1 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGTGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:3537270/1‑71 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg > 1:3466483/1‑69 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:499683/1‑71 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga > 1:616707/1‑41 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:274598/52‑1 (MQ=255) tGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcg < 1:2148317/38‑1 (MQ=255) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTa > 1:1809294/1‑36 (MQ=38) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa > 1:3894423/1‑56 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag > 1:91274/1‑38 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga > 1:874462/1‑37 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttt < 1:3354898/70‑1 (MQ=255) gTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagtcgagt > 1:2425440/1‑44 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:1912777/39‑1 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:2341495/1‑65 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:3707273/1‑39 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt < 1:2381121/65‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:1518154/1‑71 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:2691091/1‑71 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa < 1:2539659/55‑1 (MQ=255) | TAATCGACTCTTTATGACCAATCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAC > minE/2919425‑2919562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |