| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,919,516 | T→A | 30.3% | R60R (CGA→CGT) | argI ← | ornithine carbamoyltransferase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,919,516 | 0 | T | A | 30.3% | 149.7 / 79.0 | 102 | R60R (CGA→CGT) | argI | ornithine carbamoyltransferase 1 |
| Reads supporting (aligned to +/- strand): ref base T (27/44); new base A (16/15); total (43/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTT > minE/2919446‑2919583 | tCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCAt > 1:1266996/1‑71 (MQ=255) tGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCg > 1:1136939/1‑71 (MQ=255) cTGCCGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGt < 1:1150435/71‑1 (MQ=255) ccGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc > 1:1021955/1‑70 (MQ=255) ccGCTCGGCCCTAAATAGGTAACGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAc > 1:1352634/1‑70 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACGCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:1755589/69‑1 (MQ=255) cGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga < 1:47219/71‑1 (MQ=255) gCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag < 1:3292804/71‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATc < 1:3930069/61‑1 (MQ=255) cTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagt > 1:175560/1‑71 (MQ=255) tGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc > 1:764412/1‑70 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcg < 1:2916072/70‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:3227791/69‑1 (MQ=255) gggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:733628/69‑1 (MQ=255) ggCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcgag < 1:509753/71‑1 (MQ=255) cGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCt > 1:676457/1‑71 (MQ=255) gagaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGt > 1:3365593/1‑57 (MQ=255) gaTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCtttt > 1:2066507/1‑71 (MQ=255) aTAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttttt < 1:1716666/71‑1 (MQ=255) gTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGaa < 1:2915227/71‑1 (MQ=255) aaCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagtcg < 1:1874776/56‑1 (MQ=255) aCGCGCGCGCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:3790563/71‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTc < 1:635119/65‑1 (MQ=255) aCGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:902519/71‑1 (MQ=255) aCGCGAGCACCCTGGACATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:3738397/71‑1 (MQ=255) cgcgcgcgCCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCGTTTTCGAAGAt > 1:1109394/1‑70 (MQ=255) cgcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGt < 1:278775/46‑1 (MQ=255) gcgcgcgcCCTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGAt < 1:590861/69‑1 (MQ=255) gcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGAt > 1:1052411/1‑69 (MQ=255) gcgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa < 1:360040/71‑1 (MQ=255) cgAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAg < 1:2008898/71‑1 (MQ=255) gAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg > 1:1141367/1‑65 (MQ=255) gcgcccTGGTCAAATGCGGGAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcgc > 1:45921/4‑71 (MQ=255) gcgcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcgc > 1:3311355/4‑71 (MQ=255) cACCCTGGTCATATGCTGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg < 1:2405417/71‑1 (MQ=255) cACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAc < 1:764474/42‑1 (MQ=255) cACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAgc < 1:2483171/68‑1 (MQ=255) gcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa < 1:2332387/70‑1 (MQ=255) gcccTGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGa < 1:3713242/70‑1 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGTGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:3537270/1‑71 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGcgcg > 1:3466483/1‑69 (MQ=255) cccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:499683/1‑71 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga > 1:616707/1‑41 (MQ=255) ccTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCttt < 1:274598/52‑1 (MQ=255) tGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcg < 1:2148317/38‑1 (MQ=255) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTa > 1:1809294/1‑36 (MQ=38) tGGTCAAATGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGaa > 1:3894423/1‑56 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgag > 1:91274/1‑38 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTAcga > 1:874462/1‑37 (MQ=255) gTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttt < 1:3354898/70‑1 (MQ=255) gTCAAATGCGGCAACTTCGAAAGAGCAACGGGTAcgagtcgagt > 1:2425440/1‑44 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTAcgagtc < 1:1912777/39‑1 (MQ=255) tCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGAt > 1:2341495/1‑65 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTAcgagtcga > 1:3707273/1‑39 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGt < 1:2381121/65‑1 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:2691091/1‑71 (MQ=255) atatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:1518154/1‑71 (MQ=255) aaatGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGa > 1:1032924/3‑56 (MQ=255) aaatGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc < 1:1216422/69‑1 (MQ=255) aaatGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:1619642/3‑71 (MQ=255) tatGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGa < 1:2539659/55‑1 (MQ=255) atGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTAc > 1:2359750/1‑69 (MQ=255) atGCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGGGCGATGTTTTTAccg < 1:2047002/71‑2 (MQ=255) gCGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAg > 1:2088466/1‑48 (MQ=255) cGGCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGtttt > 1:1999660/1‑63 (MQ=255) ggCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCg < 1:865483/43‑1 (MQ=255) ggCAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAgcgc < 1:3763794/54‑1 (MQ=255) cAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAg < 1:573463/70‑1 (MQ=255) cAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGt < 1:2060975/71‑1 (MQ=255) cAACTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAgc < 1:1477191/71‑2 (MQ=255) aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAg < 1:2409388/43‑1 (MQ=255) aaCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGtt < 1:2673147/71‑1 (MQ=255) aaCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAgct < 1:3128023/71‑3 (MQ=255) aaCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAgct > 1:261907/1‑69 (MQ=255) aaCTTCGAAAGAGCAACGGGTACGAGTCGAGTCTTTATCGAAGATGA‑‑GCGATGTTTTTACCGGTAAGcttc > 1:1687322/1‑70 (MQ=255) aCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGttttt > 1:3109281/1‑59 (MQ=255) aCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:823246/71‑1 (MQ=255) aCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt > 1:3420171/1‑71 (MQ=255) aCTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGttt < 1:2970049/71‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCttttt < 1:3590903/35‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCttttt < 1:3797261/35‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCttttt < 1:1898847/35‑1 (MQ=255) ttCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGa > 1:245709/1‑38 (MQ=255) ttCGAAAGAGCAACGGGTACGAGTCGAGTCttttta > 1:850875/1‑35 (MQ=39) ttCGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAg < 1:1920586/40‑1 (MQ=255) cGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTgg < 1:2693425/69‑1 (MQ=255) cGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGct > 1:3577608/1‑71 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg < 1:963201/70‑4 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:1750826/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg > 1:3855338/1‑67 (MQ=255) cGAAAGAGCAACGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCGGTAAGcttctg < 1:2668452/70‑4 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGt < 1:1426296/58‑1 (MQ=255) aaGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttct > 1:1460845/1‑71 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttc < 1:2143960/71‑1 (MQ=255) gagCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttc < 1:1332095/71‑1 (MQ=255) agCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttct > 1:2330690/1‑71 (MQ=255) agCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttct < 1:3049360/71‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATg < 1:3911978/43‑1 (MQ=255) gCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttctt > 1:2547126/1‑71 (MQ=255) cATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGcttcttctt < 1:2522935/70‑1 (MQ=255) | TCTGGCTGCCGCTTGGGCCGAGATAAGTAACGCGAGCACCCTGGTCATATGCGGCAACTTCGAAAGAGCATCGGGTACGAGTCGAGTCTTTTTCGAAGATGAGCGCGATGTTTTTACCAGTGAGTTTGGCTTCTTCTT > minE/2919446‑2919583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |