| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,933,019 | G→A | 28.9% | G297G (GGC→GGT) | idnT ← | L‑idonate and D‑gluconate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,933,019 | 0 | G | A | 28.9% | 94.7 / 46.4 | 66 | G297G (GGC→GGT) | idnT | L‑idonate and D‑gluconate transporter |
| Reads supporting (aligned to +/- strand): ref base G (22/24); new base A (10/9); total (32/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
ACCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAA > minE/2932950‑2933088 | acCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAgcgc > 1:1678994/1‑71 (MQ=255) ccGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCTcc < 1:1199315/71‑1 (MQ=255) gCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCta < 1:597746/71‑1 (MQ=255) tCTACTAATACCTGCTTAAACGCTCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAg < 1:711375/70‑1 (MQ=255) tCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCtata < 1:2707431/69‑1 (MQ=255) actaATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATc > 1:1112949/1‑71 (MQ=255) aaTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCtata > 1:2591719/1‑63 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcccc < 1:3035257/70‑1 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcccc > 1:1264999/1‑70 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcccc < 1:2257496/70‑1 (MQ=255) aTACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATcccc > 1:1066493/1‑70 (MQ=255) cTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGa < 1:2617188/71‑1 (MQ=255) tGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGa > 1:2899582/1‑70 (MQ=255) cTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGgtat > 1:2886876/1‑71 (MQ=255) ttAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATc > 1:1107108/1‑71 (MQ=255) aaaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATcc < 1:2272122/70‑1 (MQ=255) aaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCta > 1:2104074/1‑49 (MQ=255) aaCGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCAt > 1:1734121/1‑71 (MQ=255) gcgcCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAt > 1:3475810/1‑71 (MQ=255) gcgcCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAt > 1:876970/1‑71 (MQ=255) cgccgccgcCAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGAtt < 1:1980700/71‑1 (MQ=255) gccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAttt > 1:924898/1‑71 (MQ=255) gccgccgccAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGAttt > 1:3830796/1‑71 (MQ=255) gccgccgccAGCGATAATAAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGAttt > 1:3053386/1‑71 (MQ=255) ccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATcccc < 1:3471265/53‑1 (MQ=255) ccgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTg < 1:90224/71‑1 (MQ=255) cgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTg < 1:3905442/70‑1 (MQ=255) cgccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGc > 1:3686884/1‑71 (MQ=255) gccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAAt > 1:3460461/1‑47 (MQ=255) gccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATc < 1:711260/48‑1 (MQ=255) gccgccAGCGATAAT‑AACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTg < 1:1241761/68‑1 (MQ=255) ccgccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTc < 1:2366617/71‑1 (MQ=255) gccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGa < 1:3407053/71‑1 (MQ=255) gccAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGa < 1:2924085/71‑1 (MQ=255) cAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGa > 1:2646539/1‑40 (MQ=255) aGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATgg > 1:3853876/1‑71 (MQ=255) cGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTg > 1:1953484/1‑71 (MQ=255) ataataAACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCg > 1:3707642/1‑71 (MQ=255) taataaACACAATCATCGCGATAGGGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTg > 1:1024448/1‑68 (MQ=255) aataaACACAATCATCGCGATAGCGCCTATAGAATCCCCAAt < 1:876088/42‑1 (MQ=255) aataaACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTg < 1:3545639/57‑1 (MQ=255) ataaACACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTcc < 1:474901/71‑1 (MQ=255) aaaCACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGc < 1:541801/65‑1 (MQ=255) aaCACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCAt < 1:170814/70‑1 (MQ=255) aaCACAATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGa < 1:301591/49‑1 (MQ=255) acacAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGt > 1:709244/1‑65 (MQ=255) acacAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTa > 1:2137422/1‑71 (MQ=255) cacaATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATgg > 1:353267/1‑59 (MQ=255) cacaATCATCGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTAc < 1:1705851/71‑1 (MQ=255) caATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATg > 1:941457/1‑44 (MQ=255) atcatcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGa > 1:3423015/1‑52 (MQ=255) atcatcGCGATAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCg < 1:1192165/71‑1 (MQ=255) tcatcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAttt > 1:958508/1‑45 (MQ=255) tcatcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTc < 1:1543831/60‑1 (MQ=255) tcatcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCa > 1:2051830/1‑62 (MQ=255) atcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGAtt > 1:2658516/1‑42 (MQ=255) tcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGc < 1:1072574/44‑1 (MQ=255) tcGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTAc < 1:3086179/63‑1 (MQ=255) gcgATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCa < 1:3923205/70‑1 (MQ=255) gcgATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAg > 1:2211055/1‑71 (MQ=255) cgATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCTATGGTGCg < 1:880916/52‑1 (MQ=255) aTAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtgt < 1:1095843/71‑1 (MQ=255) tcgcgcCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtgt < 1:806422/68‑1 (MQ=255) tAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtgt < 1:347593/70‑1 (MQ=255) tAGCACCTATAGAATCCCCAATGATATCCATGATTTGc < 1:483318/38‑1 (MQ=255) tAGCACCTATAGAATCCCCAATGATATCCATGATTTGc < 1:3899485/38‑1 (MQ=255) tAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtgt > 1:2127646/1‑70 (MQ=255) tAGCACCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAgtgt > 1:1616756/1‑70 (MQ=255) gcgcCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGtgtg > 1:1651879/1‑69 (MQ=255) gcCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGaaa > 1:2621757/1‑70 (MQ=255) | ACCGCTATCTACTAATACCTGCTTAAACGCGCCGCCGCCAGCGATAATAAACACAATCATCGCGATAGCGCCTATAGAATCCCCAATGATATCCATGATTTGCTCGATGGTGCGTCCATTACGTCGGCCCAGTGTGAAA > minE/2932950‑2933088 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |