| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,936,917 | T→C | 18.2% | K226R (AAA→AGA) | yjgB ← | predicted alcohol dehydrogenase, Zn‑dependent and NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,936,917 | 0 | T | C | 18.2% | 92.1 / 15.5 | 44 | K226R (AAA→AGA) | yjgB | predicted alcohol dehydrogenase, Zn‑dependent and NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (19/17); new base C (6/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
AAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTT > minE/2936847‑2936983 | aaaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCtt < 1:880136/71‑1 (MQ=255)aaaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCtt > 1:3371578/1‑71 (MQ=255)aaaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCtt > 1:3878720/1‑71 (MQ=255)aaaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCtt > 1:3615124/1‑71 (MQ=255) aaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCttt < 1:691414/71‑1 (MQ=255) aaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCttt < 1:1284408/71‑1 (MQ=255) aaTAGGGCTGCC‑GTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTCTCa < 1:3456229/71‑1 (MQ=255) tAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAg < 1:1516699/71‑1 (MQ=255) aGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGt > 1:2123782/1‑71 (MQ=255) ccAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTg < 1:3738308/69‑1 (MQ=255) ccAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCg > 1:2858572/1‑71 (MQ=255) aGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGa > 1:1969579/1‑71 (MQ=255) aGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTCTCAGTGCCTGCGGa > 1:3788600/1‑71 (MQ=255) tCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTCTCAGTGCCTGCGGATc > 1:2739859/1‑71 (MQ=255) gAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgc < 1:1639651/71‑1 (MQ=255) gAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgc < 1:449966/71‑1 (MQ=255) gAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgc > 1:2350762/1‑71 (MQ=255) gAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTCTCAGTGCCTGCGGATcgc > 1:2694281/1‑71 (MQ=255) cTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGc < 1:700935/70‑1 (MQ=255) cTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGc < 1:1498162/70‑1 (MQ=255) aCGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGc > 1:515154/1‑57 (MQ=255) aCGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTAtt < 1:553209/71‑1 (MQ=255) aCGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTAt < 1:103053/70‑1 (MQ=255) tGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGc > 1:110972/1‑63 (MQ=255) tGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTcgc > 1:117323/1‑68 (MQ=255) tGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTcac > 1:2670610/1‑70 (MQ=255) tGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTCTCAGTGCCTGCGGATCGCGGCt < 1:2525533/64‑1 (MQ=255) aCGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTcacca < 1:2150580/70‑1 (MQ=255) cGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACt > 1:1655222/1‑71 (MQ=255) ggTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACtt < 1:2873037/71‑1 (MQ=255) ttGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATc < 1:384529/71‑1 (MQ=255) gATAATGAGATCAAACTGCCCCGCCAGTGCTCTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCgg > 1:347786/1‑71 (MQ=255) tAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgcg > 1:2242429/1‑49 (MQ=255) aaTGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAc > 1:1297952/1‑71 (MQ=255) gaTCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATc > 1:1287691/1‑71 (MQ=255) cAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:3354982/1‑70 (MQ=255) cAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:3404720/1‑70 (MQ=255) cAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 1:1137046/1‑70 (MQ=255) aaaCTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACtt < 1:3434547/53‑1 (MQ=255) cTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATc > 1:3230742/1‑54 (MQ=255) cTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATc < 1:3708980/64‑1 (MQ=255) cTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCa > 1:2579728/1‑71 (MQ=255) ccccGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACtt > 1:2442110/1‑71 (MQ=255) ccccGCCAGTGCTCTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAc > 1:3823149/1‑56 (MQ=255) ccccGCCAGTGCTCTCAGTGCCTGAGGATCGCGGCTATTCACCACTTTATCGGCAc > 1:2216787/1‑56 (MQ=39) cGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAc > 1:1695106/1‑53 (MQ=255) gCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTg < 1:3686820/71‑1 (MQ=255) ccAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGc < 1:1098934/71‑1 (MQ=255) tGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCtt < 1:2464732/71‑1 (MQ=255) tGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCtt < 1:1066186/71‑1 (MQ=255) | AAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTT > minE/2936847‑2936983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |