| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,947,541 | G→A | 15.0% | F89F (TTC→TTT) | dnaT ← | DNA biosynthesis protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,947,541 | 0 | G | A | 15.0% | 96.3 / 8.4 | 40 | F89F (TTC→TTT) | dnaT | DNA biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base G (21/13); new base A (2/4); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACA > minE/2947472‑2947610 | tAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCg > 1:1027677/1‑70 (MQ=255) aGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCaaa > 1:3752166/1‑71 (MQ=255) cccATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCAtt > 1:990787/1‑70 (MQ=255) cccATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATg > 1:3236815/1‑71 (MQ=255) ccgccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGc > 1:2488774/1‑70 (MQ=255) ccAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACg > 1:1346603/1‑71 (MQ=255) aGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACggg < 1:555904/71‑1 (MQ=255) ggCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCa < 1:1840051/65‑1 (MQ=255) gCGGATAAATTCGGCATCGGGTTGCCAGTCCGGATACATGGCAAATTTCCCCATGGGTACAGCAACGGTAg < 1:3563904/71‑1 (MQ=255) cGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGc > 1:2492069/1‑71 (MQ=255) aaaaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCAt > 1:155886/1‑48 (MQ=255) aaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCa < 1:1294936/45‑1 (MQ=255) aTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCa > 1:1840092/1‑44 (MQ=255) tCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTa > 1:2693671/1‑49 (MQ=255) tCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTa > 1:3781657/1‑49 (MQ=255) tCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTa > 1:125885/1‑49 (MQ=255) tCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTa > 1:1292226/1‑49 (MQ=255) ggCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGtt < 1:2579747/71‑1 (MQ=255) ggCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTACGGtt < 1:2573320/71‑1 (MQ=255) aTCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCgg < 1:3848978/66‑1 (MQ=255) tCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCt > 1:2045869/1‑70 (MQ=255) tCGGGTTGCCAGTCCGGATACATGGCAAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGc < 1:3739293/63‑1 (MQ=255) gggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGa > 1:758188/1‑70 (MQ=255) gggTTGCCAGTCCGGATACATGGCAAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCgg > 1:895553/1‑63 (MQ=255) ggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGc < 1:3649385/60‑1 (MQ=255) ttGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATaga < 1:1871676/71‑1 (MQ=255) ttGCCAGTCCGGATACATGGCAAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATaga < 1:2465640/71‑1 (MQ=255) tGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCgg > 1:644068/1‑59 (MQ=255) tGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCg > 1:930045/1‑58 (MQ=255) tGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATagag > 1:3080843/1‑71 (MQ=255) aGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATagag > 1:2399594/1‑67 (MQ=255) aGTCCGGATACATGGCAAATTTCCCCATGGGTACAGCAACGGGa > 1:788113/1‑44 (MQ=255) gTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGAt > 1:2107637/1‑71 (MQ=255) tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTcc > 1:2955875/1‑57 (MQ=255) ggATACATGGCAAATTTCCCCATGGGTACAGCAACGGGAGCgg < 1:2527920/43‑1 (MQ=255) aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCt < 1:25000/53‑1 (MQ=255) aTACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTc > 1:3258203/1‑69 (MQ=255) aCATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGt < 1:917525/66‑1 (MQ=255) cATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCt < 1:1948553/40‑1 (MQ=255) tGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTg < 1:3825032/40‑1 (MQ=255) ggCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTc > 1:604028/1‑45 (MQ=255) cGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACa < 1:1181797/71‑1 (MQ=255) | TAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACA > minE/2947472‑2947610 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |