| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,952,884 | G→A | 12.9% | intergenic (‑193/+75) | leuQ ← / ← rsmC | tRNA‑Leu/16S RNA m2G1207 methylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,952,884 | 0 | G | A | 12.9% | 148.4 / 10.8 | 54 | intergenic (‑193/+75) | leuQ/rsmC | tRNA‑Leu/16S RNA m2G1207 methylase |
| Reads supporting (aligned to +/- strand): ref base G (29/18); new base A (3/4); total (32/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 | |||||||||||
GCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGC > minE/2952815‑2952951 | gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg > 1:2330371/1‑70 (MQ=255) aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt < 1:2009551/71‑1 (MQ=255) tGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAActt > 1:2109310/1‑69 (MQ=255) gTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGc < 1:2008351/71‑1 (MQ=255) atgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAg > 1:1465149/1‑71 (MQ=255) atgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAg > 1:2079663/1‑71 (MQ=255) tgcattcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAgg < 1:2309817/58‑1 (MQ=255) catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt > 1:464906/1‑71 (MQ=255) ggggCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTa < 1:2750459/70‑1 (MQ=255) cagatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAg < 1:950931/67‑1 (MQ=255) tgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt > 1:1144213/1‑70 (MQ=255) gccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt > 1:1388348/1‑69 (MQ=255) gccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt > 1:1406971/1‑69 (MQ=255) cagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttttt > 1:2483765/1‑56 (MQ=18) cagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttttt > 1:2479270/1‑56 (MQ=18) agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGc < 1:3136668/71‑1 (MQ=255) atgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGt > 1:3801982/1‑71 (MQ=255) gACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCa > 1:1922309/1‑70 (MQ=255) gACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTa < 1:3618509/36‑1 (MQ=14) gACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGt > 1:3460426/1‑40 (MQ=17) gACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGt < 1:2633690/40‑1 (MQ=17) cGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGt < 1:380235/38‑1 (MQ=17) gCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCAcgcc < 1:1581746/71‑1 (MQ=255) gCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttt < 1:2356655/45‑1 (MQ=25) cTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt < 1:1876783/47‑1 (MQ=255) ggCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAg > 1:3599125/1‑47 (MQ=255) gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCa < 1:428120/70‑1 (MQ=255) cgcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTa < 1:1344986/44‑1 (MQ=255) cgcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTa < 1:1296708/44‑1 (MQ=255) gcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAg > 1:57537/1‑35 (MQ=255) gcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa < 1:1204308/52‑1 (MQ=255) gcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAg > 1:2583045/1‑54 (MQ=255) gTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa > 1:1176388/1‑71 (MQ=255) gTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa > 1:1478570/1‑71 (MQ=255) gTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa > 1:2556783/1‑71 (MQ=255) tCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa < 1:3059670/47‑1 (MQ=255) tCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa < 1:377552/47‑1 (MQ=255) tCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa < 1:588979/70‑1 (MQ=255) ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCg > 1:2765398/1‑43 (MQ=255) ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGacac > 1:3879891/1‑71 (MQ=255) ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGacac > 1:3875302/1‑71 (MQ=255) aTCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt > 1:1878419/1‑52 (MQ=255) cTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGt > 1:1270320/1‑37 (MQ=255) cTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCAcgct < 1:1866687/56‑2 (MQ=255) cTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa > 1:3728311/1‑71 (MQ=255) cTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa > 1:3514752/1‑71 (MQ=255) tGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTAt > 1:2218443/1‑71 (MQ=255) gCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGt < 1:944342/46‑1 (MQ=255) ccTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa < 1:1741151/67‑1 (MQ=255) ccTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCg > 1:3437085/1‑71 (MQ=255) cTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCgg > 1:2896109/1‑71 (MQ=255) cTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCgg > 1:3428572/1‑71 (MQ=255) cTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCg > 1:2653060/1‑70 (MQ=255) aCGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGc > 1:1959222/1‑70 (MQ=255) cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATaa > 1:980247/1‑36 (MQ=255) | GCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGC > minE/2952815‑2952951 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |