| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,960,735 | G→C | 9.2% | intergenic (+185/+235) | yjjV → / ← yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,960,735 | 0 | G | C | 9.2% | 165.8 / 5.8 | 54 | intergenic (+185/+235) | yjjV/yjjW | predicted DNase/predicted pyruvate formate lyase activating enzyme |
| Reads supporting (aligned to +/- strand): ref base G (17/32); new base C (3/2); total (20/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGA > minE/2960666‑2960802 | tCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGt > 1:3004622/1‑71 (MQ=255) cGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGcc > 1:729297/1‑66 (MQ=40) ggCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCg > 1:1519038/1‑71 (MQ=39) tcAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGa < 1:2250453/65‑1 (MQ=255) cAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTa < 1:1158872/71‑1 (MQ=39) cAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTa < 1:2366611/71‑1 (MQ=39) ttGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtg > 1:3603993/1‑56 (MQ=255) ggCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcc < 1:522082/70‑1 (MQ=255) gcgcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGcctg < 1:2138097/71‑3 (MQ=255) cgcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGa < 1:3105339/71‑1 (MQ=255) gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg < 1:87461/56‑1 (MQ=255) gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg < 1:2353149/56‑1 (MQ=255) gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCg < 1:2178458/56‑1 (MQ=255) gcACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGAt < 1:1050671/71‑1 (MQ=255) cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGt > 1:3013132/1‑47 (MQ=255) cacaATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATa < 1:2414918/71‑1 (MQ=255) caATGCCTGATGCGACGC‑TGTCGCGTCTTATCAGGCCTACAACTAT‑TGCCGACCTGTAGGCCGGATAAgg > 1:1629148/1‑70 (MQ=11) aaTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCtgt < 1:3706744/48‑1 (MQ=255) aTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc < 1:549425/71‑1 (MQ=39) aTGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc < 1:2031189/71‑1 (MQ=39) aTGCCTGATGCGACGC‑TGTCGCGTCTTATCAGGCCTACAACTGT‑TGCCGACCTGTAGGCCGGATAAGGcac > 1:1028695/1‑69 (MQ=2) tGCCTGATGCGACGC‑TGTCGCGTCTTATCAGGCCTACAACTGT‑TGCCGACCTGTAGGCCGGATAAGGcact > 1:619685/1‑68 (MQ=0) tGCCTGATGCGACGC‑TGTCGCGTCTTATCAGGCCTACAACTGT‑TGCCGACCCGTAGGCCGGATAAGGCgc < 1:636913/70‑2 (MQ=11) ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt < 1:2200443/71‑1 (MQ=39) ccTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt < 1:227800/71‑1 (MQ=39) cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc < 1:3634524/71‑1 (MQ=39) cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc < 1:1549748/71‑1 (MQ=39) cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc < 1:2483357/71‑1 (MQ=39) cTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTc < 1:422685/71‑1 (MQ=39) tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGt < 1:2460570/38‑1 (MQ=40) tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGt < 1:113032/38‑1 (MQ=40) tGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGt < 1:117648/38‑1 (MQ=40) gCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCAcgc > 1:1879315/1‑70 (MQ=255) gACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCccgccg > 1:3668052/1‑70 (MQ=255) gCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAAc > 1:491565/1‑40 (MQ=255) gCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc > 1:204965/1‑71 (MQ=255) ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGc > 1:3877196/1‑45 (MQ=255) ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:1175304/1‑71 (MQ=255) ttGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCg > 1:1713907/1‑71 (MQ=255) ctGTCGCGTCTTATCAGGCCTACAACTGT‑TGCCGACCTGTAGGCCGGATa < 1:1403060/49‑1 (MQ=2) tCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa < 1:1050223/71‑1 (MQ=40) gTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCg > 1:3188691/1‑71 (MQ=255) aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3010247/60‑1 (MQ=255) aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:1795392/60‑1 (MQ=255) aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:1270326/60‑1 (MQ=255) aTCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCa > 1:887940/1‑71 (MQ=255) cATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGtt > 1:2011826/1‑44 (MQ=255) cATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTc > 1:2168649/1‑63 (MQ=255) aTGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:3715563/57‑1 (MQ=255) tACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGc < 1:949416/35‑1 (MQ=255) aCAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCt > 1:673345/1‑70 (MQ=255) cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCt < 1:1884132/69‑1 (MQ=255) cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa < 1:1546678/71‑1 (MQ=255) cAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGa < 1:1265104/71‑1 (MQ=255) | TCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGA > minE/2960666‑2960802 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |