Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 311,892 G→A 10.6% Y327Y (TAC→TAT acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE311,8920GA10.6% 214.5 / 12.6 75Y327Y (TAC→TATacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (30/37);  new base A (3/5);  total (33/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGA  >  minE/311822‑311956
                                                                      |                                                                
cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                                  <  1:1542576/71‑1 (MQ=255)
cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                                  >  1:1150993/1‑71 (MQ=255)
cGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                                  >  1:1162280/1‑71 (MQ=255)
 gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGt                                                                 <  1:1905565/71‑1 (MQ=255)
 gAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGt                                                                 <  1:2697793/71‑1 (MQ=255)
  aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                <  1:2483885/71‑1 (MQ=255)
  aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                >  1:2392995/1‑71 (MQ=255)
  aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                <  1:2057586/71‑1 (MQ=255)
  aGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                >  1:1894549/1‑71 (MQ=255)
   ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                                               <  1:3598567/71‑1 (MQ=255)
   ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                                               >  1:1509933/1‑71 (MQ=255)
   ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt                                                               >  1:609365/1‑71 (MQ=255)
   ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTAt                                                               >  1:3645292/1‑71 (MQ=255)
    gatgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATg                                                              <  1:3214708/71‑1 (MQ=255)
        atggCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATa                                                                >  1:3762878/4‑65 (MQ=255)
        atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt                                                           <  1:1875796/70‑1 (MQ=255)
        atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa                                                          <  1:2937811/71‑1 (MQ=255)
         tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa                                                          <  1:85513/70‑1 (MQ=255)
           gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAAc                                                       <  1:2808217/71‑1 (MQ=255)
           gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTagag                                                       <  1:1033738/71‑4 (MQ=16)
           gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTaga                                                        <  1:2746405/70‑3 (MQ=255)
            cTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGt                                                           <  1:2505299/66‑1 (MQ=255)
            cTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa                                                          <  1:3251511/67‑1 (MQ=16)
              tCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGt                                                                 >  1:2126767/1‑58 (MQ=255)
               cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa                                                     <  1:1306529/69‑1 (MQ=255)
               cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa                                                     <  1:1509709/69‑1 (MQ=255)
                gACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                                  <  1:1820918/71‑1 (MQ=255)
                  ccAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc                                                <  1:806699/71‑1 (MQ=255)
                  acAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAgg                                                             <  1:2884987/57‑1 (MQ=12)
                    aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt                                                 >  1:1361903/1‑68 (MQ=255)
                     gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa                                             >  1:3029372/1‑71 (MQ=255)
                      cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAc                                            >  1:2309036/1‑71 (MQ=255)
                       gTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa                                               >  1:1919865/1‑67 (MQ=255)
                        ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAgg                                                             >  1:2817605/1‑52 (MQ=255)
                          ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg                                                            >  1:2337074/1‑51 (MQ=255)
                          ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa                                             >  1:3565858/1‑66 (MQ=255)
                          ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa                                        >  1:3821041/1‑71 (MQ=255)
                            taCCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa                                                          >  1:1690817/2‑51 (MQ=255)
                            aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGAc                                       <  1:266319/70‑1 (MQ=255)
                              ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg                                    <  1:2267577/71‑1 (MQ=255)
                              ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACgg                                     >  1:1022388/1‑70 (MQ=255)
                                aCTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                >  1:288281/1‑41 (MQ=255)
                                 cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACa                                                      >  1:1661374/1‑50 (MQ=255)
                                 cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag                                 <  1:3082551/71‑1 (MQ=255)
                                  ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                                  <  1:3633181/37‑1 (MQ=255)
                                  ttCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCg                                                                  <  1:3714020/37‑1 (MQ=255)
                                   tCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTa                                                                >  1:2727521/1‑38 (MQ=255)
                                    cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa                                             >  1:3899604/1‑56 (MQ=255)
                                    cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaagaa                               <  1:3499436/70‑1 (MQ=255)
                                     gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                                  <  1:402317/50‑1 (MQ=255)
                                     gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                                  <  1:3302175/50‑1 (MQ=255)
                                      tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc                              >  1:1198088/1‑69 (MQ=255)
                                      tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACg                             <  1:2551473/70‑1 (MQ=255)
                                       gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAATCAATTTTCAGa                                             >  1:2399734/1‑53 (MQ=255)
                                       gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa                                             >  1:2828827/1‑53 (MQ=255)
                                        aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa                                                          >  1:2677196/1‑39 (MQ=255)
                                            gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt                                                 <  1:2773972/44‑1 (MQ=255)
                                             agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag                                 <  1:979477/59‑1 (MQ=255)
                                              gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCt                    <  1:205173/71‑1 (MQ=255)
                                               aTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt                                                  <  1:2456976/40‑1 (MQ=255)
                                               aTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCtt                   <  1:846757/71‑1 (MQ=255)
                                                ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg                                    <  1:440361/53‑1 (MQ=255)
                                                ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCtt                   <  1:3107271/70‑1 (MQ=255)
                                                 tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg                                              >  1:3763265/1‑42 (MQ=255)
                                                 tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCg                 >  1:644488/1‑71 (MQ=255)
                                                  ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg                                    >  1:806478/1‑51 (MQ=255)
                                                      cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGt            >  1:1624866/1‑71 (MQ=255)
                                                      cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGt            >  1:474727/1‑71 (MQ=255)
                                                       gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg                                              <  1:3096623/36‑1 (MQ=255)
                                                       gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACgg                                     <  1:3057978/45‑1 (MQ=255)
                                                           ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGc       <  1:366013/71‑1 (MQ=255)
                                                               tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa         <  1:338232/65‑1 (MQ=255)
                                                               tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa         <  1:2974299/65‑1 (MQ=255)
                                                               tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa         <  1:1771341/65‑1 (MQ=255)
                                                                ggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc                        <  1:1106588/49‑1 (MQ=255)
                                                                 gtgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa  <  1:1678893/70‑1 (MQ=255)
                                                                  tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt                      >  1:951494/1‑49 (MQ=255)
                                                                  tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa  >  1:2083971/1‑69 (MQ=255)
                                                                  tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa  >  1:3237093/1‑69 (MQ=255)
                                                                   gtCGTATGGGTAAACAATTTTCAGACCCGACGGgaag                                 <  1:2888826/37‑1 (MQ=255)
                                                                      gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACgg                            >  1:681618/1‑39 (MQ=255)
                                                                      |                                                                
CGAGGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGA  >  minE/311822‑311956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: