| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,895 | T→A | 10.8% | P326P (CCA→CCT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,895 | 0 | T | A | 10.8% | 172.0 / 12.4 | 65 | P326P (CCA→CCT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (24/34); new base A (2/5); total (26/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAG > minE/311825‑311965 | ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt < 1:3598567/71‑1 (MQ=255)ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:1509933/1‑71 (MQ=255)ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTAt > 1:609365/1‑71 (MQ=255)ggATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAAAGGCGTGGTGTCGTAt > 1:3645292/1‑71 (MQ=255) gatgatCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATg < 1:3214708/71‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGt < 1:1875796/70‑1 (MQ=255) atCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:2937811/71‑1 (MQ=255) tCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa < 1:85513/70‑1 (MQ=255) gCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAAc < 1:2808217/71‑1 (MQ=255) gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTagag < 1:1033738/71‑4 (MQ=16) gCTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTaga < 1:2746405/70‑3 (MQ=255) cTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGt < 1:2505299/66‑1 (MQ=255) cTTCGAACAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa < 1:3251511/67‑1 (MQ=16) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa < 1:1306529/69‑1 (MQ=255) cGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACaa < 1:1509709/69‑1 (MQ=255) gACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:1820918/71‑1 (MQ=255) ccAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTc < 1:806699/71‑1 (MQ=255) acAGCGTTTTTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAgg < 1:2884987/57‑1 (MQ=12) aGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt > 1:1361903/1‑68 (MQ=255) gCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:3029372/1‑71 (MQ=255) cGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGAc > 1:2309036/1‑71 (MQ=255) gTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCa > 1:1919865/1‑67 (MQ=255) ttttTACCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAgg > 1:2817605/1‑52 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATggg > 1:2337074/1‑51 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:3565858/1‑66 (MQ=255) ttAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGa > 1:3821041/1‑71 (MQ=255) taCCACTTCGTGAATAGAAAGCTGGACGAATGGCGTGGTGTCATAAGGGTa > 1:1690817/2‑51 (MQ=255) aaCCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGAc < 1:266319/70‑1 (MQ=255) ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg < 1:2267577/71‑1 (MQ=255) ccACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACgg > 1:1022388/1‑70 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACa > 1:1661374/1‑50 (MQ=255) cTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:3082551/71‑1 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:3899604/1‑56 (MQ=255) cGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaagaa < 1:3499436/70‑1 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:402317/50‑1 (MQ=255) gTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:3302175/50‑1 (MQ=255) tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAAc > 1:1198088/1‑69 (MQ=255) tGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACg < 1:2551473/70‑1 (MQ=255) gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAATCAATTTTCAGa > 1:2399734/1‑53 (MQ=255) gAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGa > 1:2828827/1‑53 (MQ=255) aaTAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTa > 1:2677196/1‑39 (MQ=255) gagaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAtttt < 1:2773972/44‑1 (MQ=255) agaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:979477/59‑1 (MQ=255) gaTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCt < 1:205173/71‑1 (MQ=255) aTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAAttt < 1:2456976/40‑1 (MQ=255) aTTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCtt < 1:846757/71‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg < 1:440361/53‑1 (MQ=255) ttttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCtt < 1:3107271/70‑1 (MQ=255) tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg > 1:3763265/1‑42 (MQ=255) tttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCg > 1:644488/1‑71 (MQ=255) ttCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACggg > 1:806478/1‑51 (MQ=255) cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGt > 1:1624866/1‑71 (MQ=255) cGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGt > 1:474727/1‑71 (MQ=255) gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAg < 1:3096623/36‑1 (MQ=255) gAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACgg < 1:3057978/45‑1 (MQ=255) ggCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGc < 1:366013/71‑1 (MQ=255) tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa < 1:338232/65‑1 (MQ=255) tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa < 1:2974299/65‑1 (MQ=255) tggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCa < 1:1771341/65‑1 (MQ=255) ggtgTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTcc < 1:1106588/49‑1 (MQ=255) gtgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa < 1:1678893/70‑1 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCAt > 1:951494/1‑49 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa > 1:3237093/1‑69 (MQ=255) tgtCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGa > 1:2083971/1‑69 (MQ=255) gtCGTATGGGTAAACAATTTTCAGACCCGACGGgaag < 1:2888826/37‑1 (MQ=255) gTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACgg > 1:681618/1‑39 (MQ=255) tATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACgg < 1:565006/63‑1 (MQ=255) tATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACgg < 1:2300513/63‑1 (MQ=255) aTGGGTAAACAATTTTCAGACCCGACGGGAAGAACgg > 1:3137999/1‑37 (MQ=255) tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTGCATCTTCGCCAGTTCAGCACGGATTGCCGCAg > 1:2817060/1‑71 (MQ=255) tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCa < 1:3785352/58‑1 (MQ=255) tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCg > 1:3380831/1‑68 (MQ=255) tGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCa < 1:2292815/70‑1 (MQ=255) | GGATGATCGCTTCGACCAGCGTTTTAACCACTTCGTGAATAGAGATTTTCACGAACGGCGTGGTGTCGTATGGGTAAACAATTTTCAGACCCGACGGGAAGAACGGTTCCATCTTCGCCAGTTCAGCACGGATTGCCGCAG > minE/311825‑311965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |