Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,223	T→A	12.7%	I172I (ATT→ATA)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,223	0	T	A	12.7%	228.4 / 21.4	86	I172I (ATT→ATA)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base T (45/30);  new base A (7/4);  total (52/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCA  >  minE/132155‑132293                                                                     |                                                                       aaCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                      >  1:1834634/1‑71 (MQ=255)  aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                      <  1:3546386/70‑1 (MQ=255)   cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                    >  1:2340633/1‑71 (MQ=255)    aGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGAt                                                                   >  1:2146896/1‑71 (MQ=255)      tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                 <  1:1865246/71‑1 (MQ=255)      tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                 <  1:2289256/71‑1 (MQ=255)        cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                       >  1:2128209/1‑63 (MQ=255)        cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                               >  1:1154686/1‑71 (MQ=255)        cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                               <  1:1654283/71‑1 (MQ=255)        cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                >  1:2360845/1‑70 (MQ=255)           tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                              >  1:3672365/1‑69 (MQ=255)            cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                              <  1:1911822/68‑1 (MQ=255)              cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                         >  1:1559944/1‑71 (MQ=255)               gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                        >  1:3282126/1‑71 (MQ=255)                 tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                            >  1:2689060/1‑65 (MQ=255)                 tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                      >  1:3699296/1‑71 (MQ=255)                 tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                      >  1:1378704/1‑71 (MQ=255)                      gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                  <  1:2961241/70‑1 (MQ=255)                       atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                    >  1:597024/1‑51 (MQ=255)                       atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                    >  1:788052/1‑51 (MQ=255)                         atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                              >  1:524861/1‑71 (MQ=255)                         atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                              >  1:312576/1‑71 (MQ=255)                         atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                  <  1:232113/67‑1 (MQ=255)                          tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                              >  1:3279009/1‑70 (MQ=255)                          tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                              >  1:1589625/1‑70 (MQ=255)                           cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                              <  1:820672/53‑1 (MQ=255)                            aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                           <  1:3378901/71‑1 (MQ=255)                            aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                                                          >  1:2996407/1‑56 (MQ=255)                            aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                           <  1:3916233/71‑1 (MQ=255)                            aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                           <  1:1866093/71‑1 (MQ=255)                             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                 >  1:501805/1‑64 (MQ=255)                             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                           <  1:895428/70‑1 (MQ=255)                             ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                           >  1:369444/1‑70 (MQ=255)                             ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                          >  1:3018776/1‑71 (MQ=255)                              cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                         >  1:3459369/1‑71 (MQ=255)                              cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                         >  1:1094608/1‑71 (MQ=255)                              aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                         >  1:2295944/2‑71 (MQ=255)                               aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                       >  1:3632017/1‑40 (MQ=255)                               aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                       >  1:3328833/1‑40 (MQ=255)                               aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                         >  1:1704122/1‑70 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                >  1:2133987/1‑46 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                              <  1:3474398/48‑1 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                            >  1:413424/1‑66 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                              <  1:3129806/64‑1 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                        >  1:2795204/1‑70 (MQ=255)                                tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                       <  1:822055/71‑1 (MQ=255)                                 cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                       <  1:353302/67‑1 (MQ=255)                                 cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                            >  1:1445872/1‑65 (MQ=255)                                  acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                            >  1:1816522/1‑48 (MQ=255)                                  acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                       >  1:938210/1‑69 (MQ=255)                                  acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                                              >  1:538351/1‑46 (MQ=255)                                   cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                      <  1:1085283/69‑1 (MQ=255)                                    acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                       <  1:2242153/51‑1 (MQ=255)                                    acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                   >  1:1215807/1‑71 (MQ=255)                                    acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                   >  1:3892751/1‑71 (MQ=255)                                      cGGATGACGAAATCATGCAACATCGGCGGATTGCg                                                                     >  1:192327/1‑35 (MQ=255)                                       ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                <  1:1739920/71‑1 (MQ=255)                                          tGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                <  1:1927416/36‑1 (MQ=255)                                          tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                   >  1:3322513/1‑65 (MQ=255)                                           gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                  <  1:2582517/49‑1 (MQ=255)                                           gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                  <  1:368264/49‑1 (MQ=255)                                             cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                       <  1:3826494/58‑1 (MQ=255)                                             cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                       <  1:2036055/58‑1 (MQ=255)                                              gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                           <  1:2685332/66‑1 (MQ=255)                                                aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                               <  1:3338305/47‑1 (MQ=255)                                                aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                       >  1:74025/1‑71 (MQ=255)                                                 aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                 <  1:3299476/44‑1 (MQ=255)                                                 aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                               >  1:2171812/1‑46 (MQ=255)                                                  tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                     <  1:3405915/71‑1 (MQ=255)                                                  tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                     >  1:477006/1‑71 (MQ=255)                                                   cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                    >  1:643085/1‑71 (MQ=255)                                                     tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                >  1:2239041/1‑41 (MQ=255)                                                      gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                          >  1:1559300/1‑62 (MQ=255)                                                      gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                 >  1:664640/1‑71 (MQ=255)                                                       cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                <  1:1122052/71‑1 (MQ=255)                                                        aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt               <  1:1854168/71‑1 (MQ=255)                                                           aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg            >  1:1491341/1‑71 (MQ=255)                                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg            >  1:507433/1‑70 (MQ=255)                                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc           <  1:3760758/71‑1 (MQ=255)                                                            tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc           <  1:589586/71‑1 (MQ=255)                                                               gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc        <  1:1494823/71‑1 (MQ=255)                                                                cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt       >  1:1326631/1‑71 (MQ=255)                                                                 ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg      >  1:2282189/1‑71 (MQ=255)                                                                 ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg      >  1:3369448/1‑71 (MQ=255)                                                                   aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                   <  1:2972567/40‑1 (MQ=255)                                                                   aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt    >  1:290260/1‑71 (MQ=255)                                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                       >  1:3139451/1‑50 (MQ=255)                                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa  >  1:2096639/1‑71 (MQ=255)                                                                     tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa  >  1:3660091/1‑71 (MQ=255)                                                                     |                                                                       AACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCA  >  minE/132155‑132293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑