| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,513 | T→C | 13.5% | Q120Q (CAA→CAG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,513 | 0 | T | C | 13.5% | 188.0 / 17.7 | 74 | Q120Q (CAA→CAG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (29/35); new base C (4/6); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTA > minE/312444‑312582 | gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTTCTGCTGAActtctt > 1:1784556/1‑71 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt > 1:1052482/1‑71 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt > 1:198622/1‑71 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtct < 1:1640507/70‑1 (MQ=255)gATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtct < 1:1030666/70‑1 (MQ=255) aTAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAActtctt < 1:3878093/70‑1 (MQ=255) ttaCGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc > 1:3361757/3‑71 (MQ=255) tAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGc > 1:3007291/1‑71 (MQ=255) aCGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCgg < 1:2672271/71‑1 (MQ=255) gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg > 1:419610/1‑66 (MQ=255) gCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg > 1:855360/1‑66 (MQ=255) gACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGgcagca < 1:3648748/71‑1 (MQ=255) aCAACCATCAGGAAGCTGCTGGATGACTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCaa < 1:614115/71‑1 (MQ=255) cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGgca > 1:212754/1‑62 (MQ=255) cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa < 1:3329887/71‑1 (MQ=255) cATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCa > 1:1869731/1‑71 (MQ=255) tCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCAt > 1:1543853/1‑70 (MQ=255) tCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATc > 1:2243390/1‑71 (MQ=255) aGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGc < 1:3408095/71‑1 (MQ=255) aGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGc < 1:269802/71‑1 (MQ=255) ctgctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGTCATCGCCAGCTg < 1:1460033/71‑1 (MQ=255) tgctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTg > 1:3808758/1‑70 (MQ=255) ctgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAg < 1:3829422/71‑1 (MQ=255) tgGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGgcagc < 1:2432203/50‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTg < 1:3521365/41‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGttt > 1:1551572/1‑71 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:482382/69‑1 (MQ=255) gatgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGt < 1:2680066/69‑1 (MQ=255) tgatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt > 1:2612182/1‑71 (MQ=255) gatTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCg < 1:3843372/40‑1 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt < 1:2798951/70‑1 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt < 1:3815906/70‑1 (MQ=255) atTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGtt < 1:3364271/70‑1 (MQ=255) tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCg > 1:778843/1‑53 (MQ=255) tttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt < 1:1387447/71‑1 (MQ=255) ttCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGtt < 1:2403070/64‑1 (MQ=255) tctcAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg < 1:1227821/70‑1 (MQ=255) aaCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGt < 1:1126872/62‑1 (MQ=255) aaCGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCAc < 1:2075133/69‑1 (MQ=255) cGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGc < 1:2075627/41‑1 (MQ=255) atccccTGCTGCTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTg > 1:3585923/3‑71 (MQ=255) aCCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:240322/1‑71 (MQ=255) cccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgc < 1:9975/70‑1 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc < 1:3724069/71‑1 (MQ=255) ccTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTgcgc > 1:2540559/1‑71 (MQ=255) cctgctgcTGAACCTCCTGCGGCAGCAACGTCGTGGCGAGCTGGAGTTTGTTCTGCa < 1:980077/55‑1 (MQ=255) cctgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTTCa < 1:1587391/55‑1 (MQ=255) cTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTg > 1:2035699/1‑55 (MQ=255) ttGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGc < 1:604006/55‑1 (MQ=255) tgctgcTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGa > 1:2814008/1‑70 (MQ=255) tgctgcTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGa > 1:1081387/1‑70 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGa > 1:114107/1‑70 (MQ=255) tgctgcTGAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGa > 1:69298/1‑70 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGaa > 1:3295860/1‑60 (MQ=255) gctgctGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGa < 1:923469/69‑1 (MQ=255) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat < 1:2940871/71‑1 (MQ=255) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGatat < 1:1885283/71‑1 (MQ=255) ctgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGata < 1:3637246/70‑1 (MQ=255) ctgctgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCt < 1:1467817/56‑1 (MQ=255) ctgctgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCAGCACCTGCACTTGCGCGatat < 1:2365335/71‑1 (MQ=255) tgctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCt > 1:3035932/1‑49 (MQ=255) gctgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTgcgc < 1:2523939/64‑1 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTg > 1:3640926/1‑60 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:1844797/1‑71 (MQ=255) ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:734831/1‑71 (MQ=255) tgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGat > 1:1130171/1‑65 (MQ=255) tgAACCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATcag > 1:3561527/1‑68 (MQ=255) gAACTTCTTGCGGCAGCAACTGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc > 1:846843/1‑70 (MQ=255) gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCa > 1:265643/1‑71 (MQ=255) gAACTTCTTGCGGCAGCAACGGCATCGCCAGCAGCAGtttc > 1:851633/1‑40 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc < 1:2731992/54‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAAc < 1:9925/54‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:854367/69‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:859700/69‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:849499/69‑1 (MQ=255) aaCCTCCTGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATcagg < 1:598713/69‑4 (MQ=19) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:3351000/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:3482184/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:2828559/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATc < 1:1409466/71‑1 (MQ=255) cttcttGCGGCAGCAACGGTATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:2097907/1‑69 (MQ=255) cttcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCt > 1:2098160/1‑54 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg > 1:1851380/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg < 1:1706671/69‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt < 1:1148549/70‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt < 1:1343322/70‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt < 1:2299812/70‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt > 1:2067582/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa > 1:10732/1‑71 (MQ=255) | GATAACGCCGACAACCATCAGGAAGCTGCTGGATGATTTCTCAACGCTCACCCCTTGCTGCTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTA > minE/312444‑312582 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |