| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,573 | C→A | 11.1% | A100A (GCG→GCT) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,573 | 0 | C | A | 11.1% | 337.4 / 16.6 | 108 | A100A (GCG→GCT) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (57/39); new base A (5/7); total (62/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
CTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATG > minE/312504‑312642 | ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:1844797/1‑71 (MQ=255)ctgAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCg > 1:734831/1‑71 (MQ=255) gAACTTCTTGCGGCAGCAACTGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc > 1:846843/1‑70 (MQ=255) gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCa > 1:265643/1‑71 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:859700/69‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:854367/69‑1 (MQ=255) aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc < 1:849499/69‑1 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:3482184/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:2828559/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:3351000/1‑70 (MQ=255) aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATc < 1:1409466/71‑1 (MQ=255) cttcttGCGGCAGCAACGGTATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt > 1:2097907/1‑69 (MQ=255) tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg > 1:1851380/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg < 1:1706671/69‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt < 1:1343322/70‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt < 1:2299812/70‑1 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt > 1:2067582/1‑70 (MQ=255) cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa > 1:10732/1‑71 (MQ=255) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTTCACTTGCGCGATATCAGGATCGGt < 1:94435/68‑1 (MQ=255) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtcc > 1:1607135/2‑69 (MQ=255) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtcc > 1:2054891/2‑69 (MQ=255) ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtc < 1:1321715/69‑2 (MQ=16) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:1500614/1‑69 (MQ=255) gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc > 1:2540908/1‑69 (MQ=255) cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccgg < 1:2303622/71‑3 (MQ=255) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCa < 1:1935227/70‑1 (MQ=255) cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAg < 1:3529200/71‑1 (MQ=255) ggCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa > 1:484397/1‑71 (MQ=16) gcagcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGAc < 1:352053/71‑1 (MQ=16) gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:503449/71‑1 (MQ=255) gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:2401338/71‑1 (MQ=255) cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc > 1:315622/1‑70 (MQ=255) cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt < 1:2520448/71‑1 (MQ=255) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATc > 1:3313306/1‑59 (MQ=255) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt > 1:2187233/1‑70 (MQ=255) agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt > 1:3771861/1‑70 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTc > 1:1205334/1‑70 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:3253030/71‑1 (MQ=255) gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa < 1:1549774/71‑1 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:684315/1‑71 (MQ=255) aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:962620/1‑71 (MQ=255) cGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAgg > 1:605973/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg > 1:1005238/1‑71 (MQ=255) cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAgg > 1:460897/1‑71 (MQ=255) ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt > 1:651667/1‑71 (MQ=255) gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc < 1:811430/71‑1 (MQ=255) cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa < 1:3712403/71‑1 (MQ=255) cGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt > 1:598168/1‑59 (MQ=255) gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaaa > 1:3284913/1‑62 (MQ=255) cgaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGta < 1:2853999/69‑2 (MQ=12) ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg < 1:1102180/71‑1 (MQ=255) gaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGtaa < 1:639487/70‑3 (MQ=255) gaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGtaa < 1:2916913/70‑3 (MQ=255) cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa < 1:3310785/56‑1 (MQ=255) gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg > 1:1511077/1‑56 (MQ=255) gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg > 1:633201/1‑70 (MQ=255) gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAAACTCAAAGGTCAGGGTGATCTg > 1:2604417/1‑70 (MQ=255) gaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAgg > 1:201974/2‑56 (MQ=12) cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa > 1:1096764/1‑71 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa > 1:2509207/1‑42 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc > 1:1640108/1‑71 (MQ=255) aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc > 1:1255720/1‑71 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:2747186/54‑1 (MQ=255) tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt < 1:949551/54‑1 (MQ=255) ttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg > 1:2051342/1‑37 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCa > 1:3421359/1‑35 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCa > 1:1010098/1‑35 (MQ=255) tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1017383/1‑71 (MQ=255) gTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa < 1:188869/70‑1 (MQ=255) tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAGTCAAAGGTCAggg < 1:3450658/54‑1 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc > 1:1241334/1‑61 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc > 1:3303864/1‑70 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc > 1:927970/1‑70 (MQ=255) cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg > 1:541457/1‑71 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGt > 1:54975/1‑71 (MQ=255) tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGt > 1:1424638/1‑71 (MQ=255) gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg < 1:2500555/69‑1 (MQ=255) aCCTGAACCTGCGCGATATCCGCATCAGTACCAGAc < 1:3729467/36‑1 (MQ=255) aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg > 1:1255845/1‑69 (MQ=255) aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg > 1:63853/1‑69 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa > 1:1609936/1‑61 (MQ=255) cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:3256021/1‑71 (MQ=255) tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt > 1:1926290/1‑70 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc > 1:2730804/1‑70 (MQ=255) gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:1004204/1‑71 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc < 1:1145281/69‑1 (MQ=255) aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCACGGTGATCTGCACGGTACCCGTGGAGTCa < 1:2806951/70‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:3169355/71‑1 (MQ=255) cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt < 1:2635633/71‑1 (MQ=255) gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa > 1:2965798/1‑65 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGa < 1:2305820/60‑1 (MQ=255) cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt > 1:3093525/1‑68 (MQ=255) gcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa < 1:1074987/63‑1 (MQ=255) cgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag < 1:2077407/71‑1 (MQ=255) atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa > 1:2873614/1‑71 (MQ=255) atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa > 1:10652/1‑71 (MQ=255) tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt < 1:2708622/37‑1 (MQ=255) atCCGCATCAGTACGAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa > 1:33773/1‑71 (MQ=255) atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc > 1:2586712/1‑42 (MQ=255) cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg > 1:2575464/1‑70 (MQ=255) | CTGAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATG > minE/312504‑312642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |