Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 312,575 C→G 11.3% A100P (GCG→CCG) ‡ acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE312,5750CG11.3% 298.8 / 22.3 106A100P (GCG→CCG) ‡acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base C (57/37);  new base G (5/7);  total (62/44)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 114 total aligned reads displayed.

GAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAC  >  minE/312506‑312645
                                                                     |                                                                      
gAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCa                                                                       >  1:265643/1‑71 (MQ=255)
 aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                                                                        <  1:859700/69‑1 (MQ=255)
 aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                                                                        <  1:854367/69‑1 (MQ=255)
 aaCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGc                                                                        <  1:849499/69‑1 (MQ=255)
  aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt                                                                      >  1:2828559/1‑70 (MQ=255)
  aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt                                                                      >  1:3351000/1‑70 (MQ=255)
  aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt                                                                      >  1:3482184/1‑70 (MQ=255)
  aCTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATc                                                                     <  1:1409466/71‑1 (MQ=255)
   cttcttGCGGCAGCAACGGTATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCAt                                                                      >  1:2097907/1‑69 (MQ=255)
     tcttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg                                                                   >  1:1851380/1‑70 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg                                                                   <  1:1706671/69‑1 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt                                                                  <  1:2299812/70‑1 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt                                                                  >  1:2067582/1‑70 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt                                                                  <  1:1343322/70‑1 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGt                                                                  <  1:1148549/70‑1 (MQ=255)
      cttGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa                                                                 >  1:10732/1‑71 (MQ=255)
       ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTTCACTTGCGCGATATCAGGATCGGt                                                                  <  1:94435/68‑1 (MQ=255)
       ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtcc                                                                >  1:1607135/2‑69 (MQ=255)
       ctGCGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGtcc                                                                >  1:2054891/2‑69 (MQ=255)
         gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc                                                                >  1:1500614/1‑69 (MQ=255)
         gCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTAc                                                                >  1:2540908/1‑69 (MQ=255)
          cGGCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTcccgg                                                             <  1:2303622/71‑3 (MQ=255)
          cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAg                                                             <  1:497714/71‑1 (MQ=255)
          cGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAg                                                             <  1:3529200/71‑1 (MQ=255)
           ggCAGCAACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGa                                                            >  1:484397/1‑71 (MQ=16)
            gcagcaTCGGCATCGCCAGCTGCAGTTTGTTGTGCACCTGAACCTGCGCGATATCCGCa                                                                       <  1:1993315/59‑1 (MQ=255)
            gcagcaACGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGAc                                                           <  1:352053/71‑1 (MQ=16)
            gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc                                                           <  1:503449/71‑1 (MQ=255)
            gcagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc                                                           <  1:2401338/71‑1 (MQ=255)
             cagcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAc                                                           >  1:315622/1‑70 (MQ=255)
              agcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATc                                                                     >  1:3313306/1‑59 (MQ=255)
               gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTc                                                         >  1:1205334/1‑70 (MQ=255)
               gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa                                                        <  1:3253030/71‑1 (MQ=255)
                  aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                     >  1:689156/1‑71 (MQ=255)
                  aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                     >  1:684315/1‑71 (MQ=255)
                  aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                     >  1:962620/1‑71 (MQ=255)
                   cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg                                                    >  1:1005238/1‑71 (MQ=255)
                   cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAgg                                                    >  1:460897/1‑71 (MQ=255)
                   cGGCATCGCCAGCTGCAGTTTGTTATGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg                                                    >  1:3402512/1‑71 (MQ=255)
                    ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                   >  1:651667/1‑71 (MQ=255)
                     gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc                                                  <  1:811430/71‑1 (MQ=255)
                      cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa                                                 <  1:3712403/71‑1 (MQ=255)
                         cGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACt                                                          >  1:598168/1‑59 (MQ=255)
                          gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaaa                                                      >  1:3284913/1‑62 (MQ=255)
                           cgaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGta                                            <  1:2853999/69‑2 (MQ=12)
                            gaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGtaa                                           <  1:639487/70‑3 (MQ=255)
                             aGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt                                          <  1:2366295/71‑1 (MQ=255)
                               cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa                                                       <  1:3310785/56‑1 (MQ=255)
                                 gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                     >  1:1511077/1‑56 (MQ=255)
                                 gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg                                       >  1:633201/1‑70 (MQ=255)
                                 gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAAACTCAAAGGTCAGGGTGATCTg                                       >  1:2604417/1‑70 (MQ=255)
                                  gaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAgg                                                    >  1:201974/2‑56 (MQ=12)
                                  cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                     >  1:1096764/1‑71 (MQ=255)
                                   aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTa                                                                 >  1:2509207/1‑42 (MQ=255)
                                   aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                    >  1:1640108/1‑71 (MQ=255)
                                   aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                    >  1:1255720/1‑71 (MQ=255)
                                     tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                   <  1:949551/54‑1 (MQ=255)
                                     tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                   <  1:2747186/54‑1 (MQ=255)
                                      ttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAg                                                                   >  1:2051342/1‑37 (MQ=255)
                                       tGTTCTGCACCTGAACCTGCGCGATATCCGCATCa                                                                    >  1:1010098/1‑35 (MQ=255)
                                       tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                >  1:1017383/1‑71 (MQ=255)
                                        gTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                <  1:188869/70‑1 (MQ=255)
                                          tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAGTCAAAGGTCAggg                                              <  1:3450658/54‑1 (MQ=255)
                                           cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc                                      >  1:1241334/1‑61 (MQ=255)
                                           cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc                             >  1:927970/1‑70 (MQ=255)
                                           cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc                             >  1:3303864/1‑70 (MQ=255)
                                           cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                            >  1:541457/1‑71 (MQ=255)
                                            tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGt                           >  1:1424638/1‑71 (MQ=255)
                                            tGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGt                           >  1:54975/1‑71 (MQ=255)
                                             gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                            <  1:2697790/69‑1 (MQ=255)
                                             gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                            <  1:2500555/69‑1 (MQ=255)
                                               aCCTGAACCTGCGCGATATCCGCATCAGTACCAGAc                                                           <  1:3729467/36‑1 (MQ=255)
                                               aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg                          >  1:63853/1‑69 (MQ=255)
                                               aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg                          >  1:1255845/1‑69 (MQ=255)
                                                 cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa                                                        <  1:1536096/37‑1 (MQ=255)
                                                 cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                >  1:1609936/1‑61 (MQ=255)
                                                  tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt                      >  1:1926290/1‑70 (MQ=255)
                                                   gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc                     >  1:2730804/1‑70 (MQ=255)
                                                   gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                    >  1:1004204/1‑71 (MQ=255)
                                                    aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc                     <  1:1145281/69‑1 (MQ=255)
                                                    aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCACGGTGATCTGCACGGTACCCGTGGAGTCa                    <  1:2806951/70‑1 (MQ=255)
                                                       cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                <  1:2635633/71‑1 (MQ=255)
                                                       cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                <  1:3169355/71‑1 (MQ=255)
                                                         gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                    >  1:2965798/1‑65 (MQ=255)
                                                          cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGa                        <  1:2305820/60‑1 (MQ=255)
                                                          cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                >  1:3093525/1‑68 (MQ=255)
                                                           gcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                    <  1:1074987/63‑1 (MQ=255)
                                                            cgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag           <  1:2077407/71‑1 (MQ=255)
                                                              atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa         >  1:2873614/1‑71 (MQ=255)
                                                              atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa         >  1:10652/1‑71 (MQ=255)
                                                                atCCGCATCAGTACGAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa       >  1:33773/1‑71 (MQ=255)
                                                                atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                    >  1:2586712/1‑42 (MQ=255)
                                                                   cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg     <  1:3094677/70‑1 (MQ=255)
                                                                   cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg     >  1:2575464/1‑70 (MQ=255)
                                                                    gCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa   >  1:3178849/1‑71 (MQ=255)
                                                                    gCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa   >  1:1490258/1‑71 (MQ=255)
                                                                     cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa   >  1:1144665/1‑70 (MQ=255)
                                                                     cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa   <  1:3566946/70‑1 (MQ=255)
                                                                     cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc  <  1:2717478/71‑1 (MQ=255)
                                                                     cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc  <  1:2874417/71‑1 (MQ=255)
                                                                     |                                                                      
GAACTTCTTGCGGCAGCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAC  >  minE/312506‑312645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: