Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 312,591 A→G 5.2% F94F (TTT→TTC acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE312,5910AG 5.2% 276.6 / 4.6 95F94F (TTT→TTCacrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (46/44);  new base G (2/3);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 6.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 107 total aligned reads displayed.

GCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAC  >  minE/312521‑312661
                                                                      |                                                                      
gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa                                                                        <  1:3253030/71‑1 (MQ=255)
gcaACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa                                                                        <  1:1549774/71‑1 (MQ=255)
   aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                                     >  1:962620/1‑71 (MQ=255)
   aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                                     >  1:689156/1‑71 (MQ=255)
   aCGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                                     >  1:684315/1‑71 (MQ=255)
    cGGCGTGGCGAGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAgg                                                                    >  1:605973/1‑71 (MQ=255)
    cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg                                                                    >  1:1005238/1‑71 (MQ=255)
    cGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCACGATATCCGCATCAGTACCAGACTCAAAgg                                                                    >  1:460897/1‑71 (MQ=255)
    cGGCATCGCCAGCTGCAGTTTGTTATGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAgg                                                                    >  1:3402512/1‑71 (MQ=255)
     ggCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                                   >  1:651667/1‑71 (MQ=255)
      gCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTc                                                                  <  1:811430/71‑1 (MQ=255)
       cATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCa                                                                 <  1:3712403/71‑1 (MQ=255)
           gCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaaa                                                                      >  1:3284913/1‑62 (MQ=255)
            cgaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGta                                                            <  1:2853999/69‑2 (MQ=12)
            ccAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTg                                                            <  1:1102180/71‑1 (MQ=255)
             gaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGtaa                                                           <  1:2916913/70‑3 (MQ=255)
             gaGCTGGAGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAGGTAAGGGtaa                                                           <  1:639487/70‑3 (MQ=255)
                cTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCaa                                                                       <  1:3310785/56‑1 (MQ=255)
                  gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAg                                                                     >  1:1511077/1‑56 (MQ=255)
                  gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTg                                                       >  1:633201/1‑70 (MQ=255)
                  gCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAAACTCAAAGGTCAGGGTGATCTg                                                       >  1:2604417/1‑70 (MQ=255)
                   gaGTTTGTTCTGCACCTGCACTTGCGCGATATCAGGATCGGTCCCGGACTGGAAgg                                                                    >  1:201974/2‑56 (MQ=12)
                   cAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCa                                                     >  1:1096764/1‑71 (MQ=255)
                    aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                                    >  1:1640108/1‑71 (MQ=255)
                    aGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                                    >  1:1255720/1‑71 (MQ=255)
                      tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                                   <  1:2747186/54‑1 (MQ=255)
                      tttGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGt                                                                   <  1:949551/54‑1 (MQ=255)
                        tGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                                >  1:1017383/1‑71 (MQ=255)
                         gTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                                <  1:188869/70‑1 (MQ=255)
                           tCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGAGTCAAAGGTCAggg                                                              <  1:3450658/54‑1 (MQ=255)
                            cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGc                                                      >  1:1241334/1‑61 (MQ=255)
                            cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTAccc                                             >  1:927970/1‑70 (MQ=255)
                            cTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                                            >  1:541457/1‑71 (MQ=255)
                              gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                                            <  1:2500555/69‑1 (MQ=255)
                              gCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCg                                            <  1:2697790/69‑1 (MQ=255)
                                aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg                                          >  1:63853/1‑69 (MQ=255)
                                aCCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg                                          >  1:1255845/1‑69 (MQ=255)
                                  cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCa                                                                        <  1:1536096/37‑1 (MQ=255)
                                  cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTa                                                >  1:1609936/1‑61 (MQ=255)
                                  cTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt                                      >  1:3256021/1‑71 (MQ=255)
                                   tGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGt                                      >  1:1926290/1‑70 (MQ=255)
                                    gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc                                     >  1:2730804/1‑70 (MQ=255)
                                    gAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                                    >  1:1004204/1‑71 (MQ=255)
                                     aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTc                                     <  1:1145281/69‑1 (MQ=255)
                                     aaCCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCACGGTGATCTGCACGGTACCCGTGGAGTCa                                    <  1:2806951/70‑1 (MQ=255)
                                        cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                                <  1:3169355/71‑1 (MQ=255)
                                        cTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                                <  1:2635633/71‑1 (MQ=255)
                                          gcgcGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                                    >  1:2965798/1‑65 (MQ=255)
                                           cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGa                                        <  1:2305820/60‑1 (MQ=255)
                                           cgcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGt                                >  1:3093525/1‑68 (MQ=255)
                                            gcgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCa                                    <  1:1074987/63‑1 (MQ=255)
                                             cgATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                           <  1:2077407/71‑1 (MQ=255)
                                               atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa                         >  1:2873614/1‑71 (MQ=255)
                                               atatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGa                         >  1:10652/1‑71 (MQ=255)
                                                tatCCGCATCAGTACCAGACTCAAAGGTCAGGGTGAt                                                          <  1:2708622/37‑1 (MQ=255)
                                                 atCCGCATCAGTACGAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACa                       >  1:33773/1‑71 (MQ=255)
                                                 atCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCAc                                                    >  1:2586712/1‑42 (MQ=255)
                                                    cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg                     >  1:2575464/1‑70 (MQ=255)
                                                    cGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATg                     <  1:3094677/70‑1 (MQ=255)
                                                     gCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa                   >  1:1490258/1‑71 (MQ=255)
                                                     gCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa                   >  1:3178849/1‑71 (MQ=255)
                                                      cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa                   >  1:1144665/1‑70 (MQ=255)
                                                      cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTa                   <  1:3566946/70‑1 (MQ=255)
                                                      cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc                  <  1:2874417/71‑1 (MQ=255)
                                                      cATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTAc                  <  1:2717478/71‑1 (MQ=255)
                                                       aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTaga                            <  1:919007/60‑1 (MQ=255)
                                                       aTCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTaga                            <  1:203686/60‑1 (MQ=255)
                                                          aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTg                                          >  1:1721736/1‑43 (MQ=255)
                                                          aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTagag                           >  1:1795913/1‑58 (MQ=255)
                                                          aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa              <  1:3408802/71‑1 (MQ=255)
                                                          aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa              <  1:2076713/71‑1 (MQ=255)
                                                          aGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa              <  1:2134927/71‑1 (MQ=255)
                                                           gTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGt                    <  1:1073532/64‑1 (MQ=255)
                                                            tACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAgg            <  1:640487/71‑1 (MQ=255)
                                                            tACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAgg            <  1:1200755/71‑1 (MQ=255)
                                                              ccAGACTCAAAGGTCAGGGTGATCTGCACGGTACGCGTGGAGTCACTGTTAGAGGACATGTACATCAGGtt          <  1:2155597/71‑1 (MQ=255)
                                                               cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg                                         <  1:3823769/39‑1 (MQ=255)
                                                               cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa              <  1:1891773/66‑1 (MQ=255)
                                                               cAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCa              <  1:2557084/66‑1 (MQ=255)
                                                                aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTgg                                         <  1:2330776/38‑1 (MQ=255)
                                                                aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt        <  1:2420090/71‑1 (MQ=255)
                                                                aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt        >  1:51735/1‑71 (MQ=255)
                                                                aGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt        <  1:2655081/71‑1 (MQ=255)
                                                                 gACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt        >  1:1523062/1‑70 (MQ=255)
                                                                 gACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATc       <  1:2758741/71‑1 (MQ=255)
                                                                 gACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATc       >  1:3319888/1‑71 (MQ=255)
                                                                  aCTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATc       >  1:3163857/1‑70 (MQ=255)
                                                                  aCTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATc       >  1:2039442/1‑70 (MQ=255)
                                                                  aCTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATc       >  1:93835/1‑70 (MQ=255)
                                                                   cTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACt                                  <  1:2529929/42‑1 (MQ=255)
                                                                   cTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGa     <  1:2119506/71‑1 (MQ=255)
                                                                   cTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGa     <  1:2061349/71‑1 (MQ=255)
                                                                    tCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACa                 <  1:1175603/58‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTAt        >  1:1652470/1‑65 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGa     <  1:1442003/68‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATa   <  1:1616987/70‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATa   <  1:1621565/70‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATa   <  1:1521077/70‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAc  <  1:3728057/71‑1 (MQ=255)
                                                                      aaaGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAc  >  1:3368615/1‑71 (MQ=255)
                                                                      |                                                                      
GCAACGGCATCGCCAGCTGCAGTTTGTTCTGCACCTGAACCTGCGCGATATCCGCATCAGTACCAGACTCAAAGGTCAGGGTGATCTGCACGGTACCCGTGGAGTCACTGTTAGAGGACATGTACATCAGGTTATCGATAC  >  minE/312521‑312661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: