Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,227	A→C	11.9%	I174L (ATT→CTT) ‡	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,227	0	A	C	11.9%	182.3 / 21.7	84	I174L (ATT→CTT) ‡	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base A (43/31);  new base C (6/4);  total (49/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACG  >  minE/132157‑132295                                                                       |                                                                     cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                      >  1:2340633/1‑71 (MQ=255)  aGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGAt                                                                     >  1:2146896/1‑71 (MQ=255)    tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                   <  1:2289256/71‑1 (MQ=255)    tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                   <  1:1865246/71‑1 (MQ=255)      cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                 >  1:1154686/1‑71 (MQ=255)      cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                 <  1:1654283/71‑1 (MQ=255)      cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                  >  1:2360845/1‑70 (MQ=255)         tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                >  1:3672365/1‑69 (MQ=255)          cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                <  1:1911822/68‑1 (MQ=255)            cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                           >  1:1559944/1‑71 (MQ=255)             gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                          >  1:3282126/1‑71 (MQ=255)               tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                              >  1:2689060/1‑65 (MQ=255)               tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                        >  1:1378704/1‑71 (MQ=255)               tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                        >  1:3699296/1‑71 (MQ=255)                    gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                    <  1:2961241/70‑1 (MQ=255)                     atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                      >  1:788052/1‑51 (MQ=255)                     atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                                      >  1:597024/1‑51 (MQ=255)                       atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                >  1:524861/1‑71 (MQ=255)                       atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                >  1:312576/1‑71 (MQ=255)                       atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                    <  1:232113/67‑1 (MQ=255)                        tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                >  1:3279009/1‑70 (MQ=255)                        tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                >  1:1589625/1‑70 (MQ=255)                         cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                <  1:820672/53‑1 (MQ=255)                          aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                             <  1:3378901/71‑1 (MQ=255)                          aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                                                            >  1:2996407/1‑56 (MQ=255)                          aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                             <  1:1866093/71‑1 (MQ=255)                          aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                             <  1:3916233/71‑1 (MQ=255)                           ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                   >  1:501805/1‑64 (MQ=255)                           ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                             >  1:369444/1‑70 (MQ=255)                           ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                             <  1:895428/70‑1 (MQ=255)                           ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                            >  1:3018776/1‑71 (MQ=255)                            cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                           >  1:1094608/1‑71 (MQ=255)                            cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                           >  1:3459369/1‑71 (MQ=255)                            aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                           >  1:2295944/2‑71 (MQ=255)                             aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                           >  1:1704122/1‑70 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                  >  1:2133987/1‑46 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                <  1:3474398/48‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                              >  1:413424/1‑66 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                <  1:3129806/64‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                          >  1:2795204/1‑70 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                         <  1:822055/71‑1 (MQ=255)                               cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                         <  1:353302/67‑1 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                              >  1:1445872/1‑65 (MQ=255)                                acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                              >  1:1816522/1‑48 (MQ=255)                                acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                         >  1:938210/1‑69 (MQ=255)                                acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                                                >  1:538351/1‑46 (MQ=255)                                 cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                        <  1:1085283/69‑1 (MQ=255)                                  acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                         <  1:2242153/51‑1 (MQ=255)                                  acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                     >  1:3892751/1‑71 (MQ=255)                                  acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                     >  1:1215807/1‑71 (MQ=255)                                     ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                  <  1:1739920/71‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                  <  1:1927416/36‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                     >  1:3322513/1‑65 (MQ=255)                                         gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                    <  1:2582517/49‑1 (MQ=255)                                         gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                    <  1:368264/49‑1 (MQ=255)                                           cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                         <  1:3826494/58‑1 (MQ=255)                                           cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                         <  1:2036055/58‑1 (MQ=255)                                            gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                             <  1:2685332/66‑1 (MQ=255)                                              aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                 <  1:3338305/47‑1 (MQ=255)                                              aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                         >  1:74025/1‑71 (MQ=255)                                               aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                   <  1:3299476/44‑1 (MQ=255)                                               aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                 >  1:2171812/1‑46 (MQ=255)                                                tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                       <  1:3405915/71‑1 (MQ=255)                                                tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                       >  1:477006/1‑71 (MQ=255)                                                 cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                      >  1:643085/1‑71 (MQ=255)                                                   tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                  >  1:2239041/1‑41 (MQ=255)                                                    gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                            >  1:1559300/1‑62 (MQ=255)                                                    gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                   >  1:664640/1‑71 (MQ=255)                                                     cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                  <  1:1122052/71‑1 (MQ=255)                                                      aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                 <  1:1854168/71‑1 (MQ=255)                                                         aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg              >  1:1491341/1‑71 (MQ=255)                                                          tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg              >  1:507433/1‑70 (MQ=255)                                                          tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc             <  1:3760758/71‑1 (MQ=255)                                                          tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc             <  1:589586/71‑1 (MQ=255)                                                             gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc          <  1:1494823/71‑1 (MQ=255)                                                              cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt         >  1:1326631/1‑71 (MQ=255)                                                               ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg        >  1:3369448/1‑71 (MQ=255)                                                               ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg        >  1:2282189/1‑71 (MQ=255)                                                                 aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                     <  1:2972567/40‑1 (MQ=255)                                                                 aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt      >  1:290260/1‑71 (MQ=255)                                                                   tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                         >  1:3139451/1‑50 (MQ=255)                                                                   tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa    >  1:3660091/1‑71 (MQ=255)                                                                   tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa    >  1:2096639/1‑71 (MQ=255)                                                                     cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                       >  1:3594428/1‑50 (MQ=255)                                                                     cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc   <  1:1619129/70‑1 (MQ=255)                                                                     cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc   <  1:1040486/70‑1 (MQ=255)                                                                     cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg  >  1:2472892/1‑71 (MQ=255)                                                                       aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                  <  1:2496046/53‑1 (MQ=255)                                                                       |                                                                     CAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACG  >  minE/132157‑132295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑