| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 312,696 | G→A | 20.4% | D59D (GAC→GAT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 312,696 | 0 | G | A | 20.4% | 188.4 / 40.4 | 88 | D59D (GAC→GAT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (27/43); new base A (11/7); total (38/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
ACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGT > minE/312627‑312764 | aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg < 1:1065346/70‑1 (MQ=255)aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg < 1:1075688/70‑1 (MQ=255)aCTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCgtg < 1:3068805/70‑1 (MQ=255) tGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTcc > 1:1747006/1‑71 (MQ=255) tGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTcc > 1:2684014/1‑71 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTcc < 1:3427591/69‑1 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg < 1:2326700/71‑1 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg < 1:2407123/71‑1 (MQ=255) ttAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg > 1:1333341/1‑71 (MQ=255) gtggagGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTg < 1:1630433/68‑1 (MQ=25) gtggagGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTg > 1:3111717/4‑71 (MQ=25) gtggagGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTg < 1:512134/68‑1 (MQ=25) tAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTg > 1:290465/1‑70 (MQ=255) agagGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGc < 1:2878758/70‑1 (MQ=255) agagGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCa > 1:541248/1‑71 (MQ=255) agagGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCa > 1:999350/1‑71 (MQ=255) agGACATATACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCAcg > 1:3447145/1‑70 (MQ=255) ggACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTg < 1:1190008/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:248404/71‑1 (MQ=255) cATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:1026371/71‑1 (MQ=255) cATGTACATCAGCTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGttt < 1:1211857/71‑1 (MQ=255) tGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc < 1:1535085/71‑1 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc > 1:612396/1‑69 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc > 1:1247704/1‑69 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCg > 1:352436/1‑70 (MQ=255) tACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGc < 1:1873206/71‑1 (MQ=255) aCATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCa > 1:2709462/1‑71 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCTCAt < 1:1813843/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:1594504/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:3837007/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:265665/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:514134/71‑1 (MQ=255) cATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt < 1:3274708/71‑1 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCAt > 1:964414/1‑70 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc < 1:272665/71‑1 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc < 1:3355219/71‑1 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc < 1:1739761/71‑1 (MQ=255) aTCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCAt > 1:1037871/1‑70 (MQ=37) tCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCa < 1:53633/71‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGc > 1:2659004/1‑65 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGTATCAg < 1:480624/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAg < 1:405278/70‑1 (MQ=255) aGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAgc > 1:3362124/1‑71 (MQ=37) ttATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc > 1:2431728/1‑65 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc > 1:1697515/1‑64 (MQ=255) tATCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGccc > 1:714867/1‑70 (MQ=255) aTCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTc < 1:1077446/58‑1 (MQ=255) tCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCAcgg < 1:2761090/52‑3 (MQ=37) tCGATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCAcgg < 1:1481597/52‑3 (MQ=37) cGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:2923637/1‑71 (MQ=255) cGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:1807409/1‑71 (MQ=255) gATACCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCcgga < 1:3615062/70‑2 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg < 1:2667872/66‑1 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCg < 1:24974/66‑1 (MQ=255) aTACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTa < 1:858137/71‑1 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTc > 1:2316299/1‑39 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTc > 1:1137373/1‑39 (MQ=255) aCCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCAcg > 1:588285/1‑45 (MQ=37) aCCGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCAcg > 1:1740611/1‑45 (MQ=37) ccGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTagga < 1:1470941/71‑1 (MQ=255) ccGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCa > 1:893231/1‑57 (MQ=255) ccGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCa > 1:683653/1‑57 (MQ=255) ccGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagtt > 1:3706071/1‑69 (MQ=25) cGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagttt < 1:1412321/71‑4 (MQ=255) cGTTCATATTCTGTTCGATAACCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCCGGATagttt < 1:173351/71‑4 (MQ=255) gTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc < 1:3919259/54‑1 (MQ=255) cATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc > 1:1537787/1‑51 (MQ=255) atatTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc < 1:1086841/56‑1 (MQ=255) atTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATc < 1:43547/48‑1 (MQ=255) ttCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGAt < 1:3928209/71‑1 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtttt > 1:346902/1‑39 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGtttt > 1:575913/1‑39 (MQ=255) cTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCTGGGTAGGAGGCGGAGATCg > 1:2437973/1‑71 (MQ=255) ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc < 1:1009101/48‑1 (MQ=255) ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGcc < 1:1310219/48‑1 (MQ=255) ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCg < 1:2771027/68‑1 (MQ=255) ttCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt < 1:315028/69‑1 (MQ=255) tCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTAc > 1:3030298/1‑71 (MQ=255) cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGTGCCGGGGTAGGAGGCGGAGATCGTTAc > 1:1280558/1‑70 (MQ=255) cGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACt > 1:348681/1‑71 (MQ=255) aTAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTagga < 1:3662500/53‑1 (MQ=255) aTAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGc < 1:3503577/71‑1 (MQ=255) aaCCTGCGTCACCGTATCCTGCACGGTCTGCGCATCAGCGCCcggat > 1:1763248/1‑45 (MQ=16) cTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggcg > 1:3112881/1‑71 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCcggcgg < 1:56683/69‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGt < 1:163203/70‑1 (MQ=255) tgtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGt < 1:575948/70‑1 (MQ=255) gtCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGt < 1:1452694/56‑1 (MQ=255) aCCGTGTCCTGCACTGTTTTCGCATCAGCGCCgggg > 1:1440289/1‑36 (MQ=255) cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCg < 1:3818560/71‑1 (MQ=255) cGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCg < 1:386248/71‑1 (MQ=255) gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGt < 1:3062016/71‑1 (MQ=255) gtgtCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGt < 1:3424427/71‑1 (MQ=255) | ACTGTTAGAGGACATGTACATCAGGTTATCGATACCGTTCATATTCTGTTCGATAACCTGTGTCACCGTGTCCTGCACTGTTTTCGCATCAGCGCCGGGGTAGGAGGCGGAGATCGTTACTGCCGGCGGTGCAATCGT > minE/312627‑312764 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |