Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,227	G→A	12.0%	L288L (CTG→TTG)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,227	0	G	A	12.0%	155.0 / 10.1	58	L288L (CTG→TTG)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base A (4/3);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGT  >  minE/313159‑313293                                                                     |                                                                   ttGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGgcag                                                                    <  1:172148/69‑1 (MQ=255)        aCTAAAATAGCGTTTGGATTAAGCCCTTCTTCAAGACGTGCGCGCACGAACATACCCGGCAGCAGAGtgtg                                                           <  1:2866310/71‑1 (MQ=255)         cTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGa                                                          >  1:2653616/1‑71 (MQ=255)            aaaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                       >  1:2839355/1‑71 (MQ=255)            aaaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                       >  1:1643598/1‑71 (MQ=255)             aaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                       <  1:3018348/70‑1 (MQ=255)                  cGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTc                                                 <  1:1249372/71‑1 (MQ=255)                    tttGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCgg                                               >  1:2870226/1‑71 (MQ=255)                      tGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGtgtg                                                           >  1:2929418/1‑57 (MQ=255)                      tGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCggg                                              <  1:3586547/70‑1 (MQ=255)                       ggATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGa                                             <  1:1256628/70‑1 (MQ=255)                        gATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGaa                                            <  1:1484255/70‑1 (MQ=255)                         aTTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCgg                                               >  1:782222/1‑66 (MQ=255)                         aTTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                          <  1:2252637/71‑1 (MQ=255)                          ttAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                          <  1:3160175/70‑1 (MQ=255)                              gCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAgcg                                     >  1:1054775/1‑71 (MQ=255)                                ccTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAg                                       >  1:2731923/1‑67 (MQ=255)                                ccTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGcgcg                                   >  1:2621553/1‑71 (MQ=255)                                 cttcttCCAGACGTGCGCGCACGAACATACCCGGAAGCAGAGTGTGATCCGGGTTCGGGAAGATAGcgcg                                   >  1:2840704/1‑70 (MQ=255)                                    cttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                       >  1:2993819/1‑47 (MQ=255)                                    cttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAg                                <  1:3262776/70‑1 (MQ=255)                                     ttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAggg                              >  1:38203/1‑71 (MQ=255)                                      tcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGt                             >  1:1407321/1‑71 (MQ=255)                                       ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGaa                                            <  1:1627026/55‑1 (MQ=255)                                       ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAggg                              <  1:1014023/69‑1 (MQ=255)                                       ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGt                             <  1:1319217/70‑1 (MQ=255)                                        cAGACGTGCGCGCACGAACATACCCGGCAGCAGTGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTg                            <  1:177122/70‑1 (MQ=255)                                        cAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTg                            <  1:1392199/70‑1 (MQ=255)                                         atccGTGCACGCACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTAt                          >  1:1440171/4‑71 (MQ=255)                                            cGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGa                       >  1:2506084/1‑71 (MQ=255)                                            cGTGCACGCACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGa                       <  1:2558874/71‑1 (MQ=255)                                             gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                      >  1:2161671/1‑71 (MQ=255)                                             gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                      >  1:963958/1‑71 (MQ=255)                                             gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                      <  1:2972675/71‑1 (MQ=255)                                                cgcgcACGAACATACCCGGCAGCAGAGTGTGATcc                                                       <  1:3899538/35‑1 (MQ=255)                                                cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAccc                    <  1:347168/70‑1 (MQ=255)                                                cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAccc                    <  1:342478/70‑1 (MQ=255)                                                cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCa                   <  1:968922/71‑1 (MQ=255)                                                 gcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAg                  <  1:2866077/71‑1 (MQ=255)                                                   gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                >  1:2520459/1‑71 (MQ=255)                                                   gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                >  1:2524937/1‑71 (MQ=255)                                                   gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                >  1:2547432/1‑71 (MQ=255)                                                    cACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTgg               <  1:2958123/71‑1 (MQ=255)                                                     aCGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGt              <  1:1805484/71‑1 (MQ=255)                                                       gAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCt            >  1:642806/1‑71 (MQ=255)                                                       aaaCATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGag                      <  1:1671618/60‑2 (MQ=255)                                                        aaCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGa                           >  1:378178/1‑55 (MQ=255)                                                        aaCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTg           <  1:3167200/71‑1 (MQ=255)                                                        aaCATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGcacg                                   <  1:1086704/47‑1 (MQ=255)                                                         aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                          <  1:1570178/39‑1 (MQ=255)                                                         aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                      >  1:3023332/1‑59 (MQ=255)                                                         aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGa          >  1:602593/1‑71 (MQ=255)                                                         aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGa          >  1:1242164/1‑71 (MQ=255)                                                           aTACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATa                                        >  1:2628368/1‑39 (MQ=255)                                                            tACCCGTCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGt                                  >  1:3479220/1‑44 (MQ=255)                                                             aCCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATa                                        >  1:1083030/1‑37 (MQ=255)                                                                 ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACgg   >  1:1445506/1‑70 (MQ=255)                                                                 ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACg    >  1:3948476/1‑69 (MQ=255)                                                                 ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGt  <  1:688501/71‑1 (MQ=255)                                                                     |                                                                   TTGCGGGACTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGT  >  minE/313159‑313293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑