Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,237	A→T	10.7%	D284E (GAT→GAA) ‡	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,237	0	A	T	10.7%	170.7 / 11.1	65	D284E (GAT→GAA) ‡	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base T (4/3);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATT  >  minE/313167‑313307                                                                       |                                                                       cTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGa                                                                        >  1:2653616/1‑71 (MQ=255)    aaaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                                     >  1:2839355/1‑71 (MQ=255)    aaaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                                     >  1:1643598/1‑71 (MQ=255)     aaTAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                                     <  1:3018348/70‑1 (MQ=255)          cGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTc                                                               <  1:1249372/71‑1 (MQ=255)            tttGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCgg                                                             >  1:2870226/1‑71 (MQ=255)              tGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCggg                                                            <  1:3586547/70‑1 (MQ=255)               ggATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGa                                                           <  1:1256628/70‑1 (MQ=255)                gATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGaa                                                          <  1:1484255/70‑1 (MQ=255)                 aTTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCgg                                                             >  1:782222/1‑66 (MQ=255)                 aTTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                                        <  1:2252637/71‑1 (MQ=255)                  ttAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                                        <  1:3160175/70‑1 (MQ=255)                      gCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAgcg                                                   >  1:1054775/1‑71 (MQ=255)                        ccTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAg                                                     >  1:2731923/1‑67 (MQ=255)                        ccTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGcgcg                                                 >  1:2621553/1‑71 (MQ=255)                         cttcttCCAGACGTGCGCGCACGAACATACCCGGAAGCAGAGTGTGATCCGGGTTCGGGAAGATAGcgcg                                                 >  1:2840704/1‑70 (MQ=255)                            cttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATcc                                                                     >  1:2993819/1‑47 (MQ=255)                            cttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAg                                              <  1:3262776/70‑1 (MQ=255)                             ttcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAggg                                            >  1:38203/1‑71 (MQ=255)                              tcCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGt                                           >  1:1407321/1‑71 (MQ=255)                               ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGaa                                                          <  1:1627026/55‑1 (MQ=255)                               ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAggg                                            <  1:1014023/69‑1 (MQ=255)                               ccAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGt                                           <  1:1319217/70‑1 (MQ=255)                                cAGACGTGCGCGCACGAACATACCCGGCAGCAGTGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTg                                          <  1:177122/70‑1 (MQ=255)                                cAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTg                                          <  1:1392199/70‑1 (MQ=255)                                 atccGTGCACGCACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTAt                                        >  1:1440171/4‑71 (MQ=255)                                    cGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGa                                     >  1:2506084/1‑71 (MQ=255)                                    cGTGCACGCACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGa                                     <  1:2558874/71‑1 (MQ=255)                                     gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                                    <  1:2972675/71‑1 (MQ=255)                                     gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                                    >  1:2161671/1‑71 (MQ=255)                                     gTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                                    >  1:963958/1‑71 (MQ=255)                                        cgcgcACGAACATACCCGGCAGCAGAGTGTGATcc                                                                     <  1:3899538/35‑1 (MQ=255)                                        cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAccc                                  <  1:342478/70‑1 (MQ=255)                                        cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAccc                                  <  1:347168/70‑1 (MQ=255)                                        cgcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCa                                 <  1:968922/71‑1 (MQ=255)                                         gcgcACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAg                                <  1:2866077/71‑1 (MQ=255)                                           gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                              >  1:2547432/1‑71 (MQ=255)                                           gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                              >  1:2524937/1‑71 (MQ=255)                                           gcACAAACATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGAGCCGGTg                              >  1:2520459/1‑71 (MQ=255)                                            cACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTgg                             <  1:2958123/71‑1 (MQ=255)                                             aCGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGt                            <  1:1805484/71‑1 (MQ=255)                                               gAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCt                          >  1:642806/1‑71 (MQ=255)                                               aaaCATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGCACGTAGGGTTATGGag                                    <  1:1671618/60‑2 (MQ=255)                                                aaCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGa                                         >  1:378178/1‑55 (MQ=255)                                                aaCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTg                         <  1:3167200/71‑1 (MQ=255)                                                aaCATACCCGGCAAAAGCGTATGTTGCGGGTTAGGGAAGACAGcacg                                                 <  1:1086704/47‑1 (MQ=255)                                                 aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGa                                                        <  1:1570178/39‑1 (MQ=255)                                                 aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAc                                    >  1:3023332/1‑59 (MQ=255)                                                 aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGa                        >  1:602593/1‑71 (MQ=255)                                                 aCATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGa                        >  1:1242164/1‑71 (MQ=255)                                                   aTACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATa                                                      >  1:2628368/1‑39 (MQ=255)                                                    tACCCGTCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGt                                                >  1:3479220/1‑44 (MQ=255)                                                     aCCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATa                                                      >  1:1083030/1‑37 (MQ=255)                                                         ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACgg                 >  1:1445506/1‑70 (MQ=255)                                                         ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACg                  >  1:3948476/1‑69 (MQ=255)                                                         ggCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGt                <  1:688501/71‑1 (MQ=255)                                                              cagAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGt           >  1:3223652/1‑71 (MQ=255)                                                              cagAGTGTGATCCGGGTTCGGGAAGATAGAGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGt           >  1:12574/1‑71 (MQ=255)                                                                gagTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAAc             >  1:954253/1‑67 (MQ=255)                                                                 agTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGt           >  1:1175292/1‑68 (MQ=255)                                                                 agTGTGATCCAGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCag        <  1:2877761/71‑1 (MQ=255)                                                                  gtgtGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATc                      <  1:1593406/56‑1 (MQ=255)                                                                    gtgATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCaga       >  1:1108409/1‑69 (MQ=255)                                                                     tgATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGa                                     >  1:1712043/1‑38 (MQ=255)                                                                     tgATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGAcc                                   <  1:2816754/40‑1 (MQ=255)                                                                      gATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAAt   >  1:1660834/1‑71 (MQ=255)                                                                       aTCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCa                     <  1:3886428/52‑1 (MQ=255)                                                                       aTCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCa                     <  1:3697904/52‑1 (MQ=255)                                                                       aTCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAAtt  >  1:2336518/1‑71 (MQ=255)                                                                       aTCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAAt   <  1:1027793/70‑1 (MQ=255)                                                                       |                                                                       CTAAAATAGCGTTTGGATTAAGCCCTTCTTCCAGACGTGCGCGCACGAACATACCCGGCAGCAGAGTGTGATCCGGGTTCGGGAAGATAGCGCGTAGGGTGATAGACCCAGTGGTCTGATCAACGGTAACGTCAGAGAATT  >  minE/313167‑313307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑