Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,229	T→G	13.0%	I174M (ATT→ATG) ‡	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,229	0	T	G	13.0%	161.1 / 23.7	77	I174M (ATT→ATG) ‡	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base T (39/28);  new base G (6/4);  total (45/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

TCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCT  >  minE/132160‑132297                                                                      |                                                                     tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                     <  1:1865246/71‑1 (MQ=255) tCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtc                                                                     <  1:2289256/71‑1 (MQ=255)   cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                   >  1:1154686/1‑71 (MQ=255)   cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                   <  1:1654283/71‑1 (MQ=255)   cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                    >  1:2360845/1‑70 (MQ=255)      tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                  >  1:3672365/1‑69 (MQ=255)       cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                  <  1:1911822/68‑1 (MQ=255)         cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                             >  1:1559944/1‑71 (MQ=255)          gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                            >  1:3282126/1‑71 (MQ=255)            tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                                >  1:2689060/1‑65 (MQ=255)            tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                          >  1:3699296/1‑71 (MQ=255)            tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                          >  1:1378704/1‑71 (MQ=255)                 gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                      <  1:2961241/70‑1 (MQ=255)                    atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                  >  1:524861/1‑71 (MQ=255)                    atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                  >  1:312576/1‑71 (MQ=255)                    atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                      <  1:232113/67‑1 (MQ=255)                     tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                  >  1:3279009/1‑70 (MQ=255)                     tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                  >  1:1589625/1‑70 (MQ=255)                      cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                  <  1:820672/53‑1 (MQ=255)                       aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                               <  1:3378901/71‑1 (MQ=255)                       aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                                                              >  1:2996407/1‑56 (MQ=255)                       aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                               <  1:1866093/71‑1 (MQ=255)                       aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                               <  1:3916233/71‑1 (MQ=255)                        ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                     >  1:501805/1‑64 (MQ=255)                        ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                               <  1:895428/70‑1 (MQ=255)                        ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                               >  1:369444/1‑70 (MQ=255)                        ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                              >  1:3018776/1‑71 (MQ=255)                         cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                             >  1:3459369/1‑71 (MQ=255)                         cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                             >  1:1094608/1‑71 (MQ=255)                         aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                             >  1:2295944/2‑71 (MQ=255)                          aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                             >  1:1704122/1‑70 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                    >  1:2133987/1‑46 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                  <  1:3474398/48‑1 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                >  1:413424/1‑66 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                  <  1:3129806/64‑1 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                            >  1:2795204/1‑70 (MQ=255)                           tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                           <  1:822055/71‑1 (MQ=255)                            cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                           <  1:353302/67‑1 (MQ=255)                            cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                >  1:1445872/1‑65 (MQ=255)                             acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                                >  1:1816522/1‑48 (MQ=255)                             acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                           >  1:938210/1‑69 (MQ=255)                             acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                                                  >  1:538351/1‑46 (MQ=255)                              cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                          <  1:1085283/69‑1 (MQ=255)                               acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                           <  1:2242153/51‑1 (MQ=255)                               acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                       >  1:1215807/1‑71 (MQ=255)                               acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                       >  1:3892751/1‑71 (MQ=255)                                  ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                    <  1:1739920/71‑1 (MQ=255)                                     tGACGAAATCATGCAACATCGGCGGATTGCGATtct                                                                    <  1:1927416/36‑1 (MQ=255)                                     tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                       >  1:3322513/1‑65 (MQ=255)                                      gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                      <  1:2582517/49‑1 (MQ=255)                                      gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                      <  1:368264/49‑1 (MQ=255)                                        cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                           <  1:3826494/58‑1 (MQ=255)                                        cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                           <  1:2036055/58‑1 (MQ=255)                                         gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                               <  1:2685332/66‑1 (MQ=255)                                           aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                   <  1:3338305/47‑1 (MQ=255)                                           aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                           >  1:74025/1‑71 (MQ=255)                                            aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                     <  1:3299476/44‑1 (MQ=255)                                            aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                   >  1:2171812/1‑46 (MQ=255)                                             tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                         <  1:3405915/71‑1 (MQ=255)                                             tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                         >  1:477006/1‑71 (MQ=255)                                              cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                        >  1:643085/1‑71 (MQ=255)                                                tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                    >  1:2239041/1‑41 (MQ=255)                                                 gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                              >  1:1559300/1‑62 (MQ=255)                                                 gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                     >  1:664640/1‑71 (MQ=255)                                                  cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                    <  1:1122052/71‑1 (MQ=255)                                                   aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                   <  1:1854168/71‑1 (MQ=255)                                                      aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                >  1:1491341/1‑71 (MQ=255)                                                       tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                >  1:507433/1‑70 (MQ=255)                                                       tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc               <  1:3760758/71‑1 (MQ=255)                                                       tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc               <  1:589586/71‑1 (MQ=255)                                                          gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc            <  1:1494823/71‑1 (MQ=255)                                                           cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt           >  1:1326631/1‑71 (MQ=255)                                                            ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg          >  1:3369448/1‑71 (MQ=255)                                                            ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg          >  1:2282189/1‑71 (MQ=255)                                                              aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                       <  1:2972567/40‑1 (MQ=255)                                                              aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt        >  1:290260/1‑71 (MQ=255)                                                                tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                           >  1:3139451/1‑50 (MQ=255)                                                                tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa      >  1:3660091/1‑71 (MQ=255)                                                                tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa      >  1:2096639/1‑71 (MQ=255)                                                                  cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                         >  1:3594428/1‑50 (MQ=255)                                                                  cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc     <  1:1040486/70‑1 (MQ=255)                                                                  cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc     <  1:1619129/70‑1 (MQ=255)                                                                  cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg    >  1:2472892/1‑71 (MQ=255)                                                                    aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                    <  1:2496046/53‑1 (MQ=255)                                                                     ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                     <  1:66698/51‑1 (MQ=255)                                                                     ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt  >  1:2838467/1‑70 (MQ=255)                                                                     ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt  >  1:960573/1‑70 (MQ=255)                                                                      tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                <  1:3322401/39‑1 (MQ=255)                                                                      |                                                                     TCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCT  >  minE/132160‑132297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑