Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 313,462 A→G 8.1% L209L (CTT→CTC acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE313,4620AG 8.1% 240.3 / 10.0 86L209L (CTT→CTCacrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (38/41);  new base G (5/2);  total (43/43)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAC  >  minE/313394‑313531
                                                                    |                                                                     
ttCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAg                                                                     >  1:1179310/1‑71 (MQ=255)
ttCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAg                                                                     >  1:3535527/1‑71 (MQ=255)
  cGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGt                                                                    <  1:2771688/70‑1 (MQ=255)
   gCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTTGGTCACATCAACGTAGATCGGATCAAGTtg                                                                  <  1:381997/71‑1 (MQ=255)
       gTTCCTGTTTCAGGAGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGt                                                                    <  1:2785126/65‑1 (MQ=255)
        ttCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                             >  1:2278772/1‑71 (MQ=255)
          ccTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCAc                                                            <  1:450876/70‑1 (MQ=255)
           cTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                          <  1:2646348/71‑1 (MQ=255)
            tGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                  >  1:3620079/1‑62 (MQ=255)
            tGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                  >  1:2638386/1‑62 (MQ=255)
             gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                        >  1:2545727/1‑71 (MQ=255)
             gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                        >  1:1695547/1‑71 (MQ=255)
              tttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTg                                                       >  1:3390775/1‑71 (MQ=255)
               ttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGc                                                      >  1:975161/1‑71 (MQ=255)
                tCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtgctg                                                               >  1:2865771/1‑61 (MQ=255)
                 cAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTATATCGGATCAAGTTGCTGCACgg                                                          <  1:1447814/65‑1 (MQ=255)
                  aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                   <  1:3427590/71‑1 (MQ=255)
                  aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                   <  1:3770735/71‑1 (MQ=255)
                  aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                   >  1:423130/1‑71 (MQ=255)
                      gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                               <  1:953390/71‑1 (MQ=255)
                      gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                               <  1:889669/71‑1 (MQ=255)
                       cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                               <  1:2702468/70‑1 (MQ=255)
                       cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                              <  1:358370/71‑1 (MQ=255)
                        aGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                         <  1:2928788/59‑1 (MQ=255)
                        aGGAAGTCGTTGCTGGACTGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                         <  1:610422/58‑1 (MQ=255)
                         ggAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                            <  1:1496254/71‑1 (MQ=255)
                           aaGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGc                                          <  1:3402261/71‑1 (MQ=255)
                            aGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                  >  1:3157123/1‑46 (MQ=255)
                              tCGTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGcc                                                     >  1:1482115/1‑57 (MQ=255)
                              tCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                  <  1:3384712/44‑1 (MQ=255)
                                gTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAgttc                                                >  1:3406562/1‑57 (MQ=255)
                                gTTGCTGGACTGGGTCATATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                          <  1:1763547/50‑1 (MQ=255)
                                gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                          <  1:3638478/50‑1 (MQ=255)
                                gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTg                                       >  1:411650/1‑69 (MQ=255)
                                gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGAc                                     >  1:788561/1‑71 (MQ=255)
                                 ttGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                        >  1:2392360/1‑51 (MQ=255)
                                  tGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                             >  1:469065/1‑45 (MQ=255)
                                  tGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                        >  1:3839957/1‑50 (MQ=255)
                                   gCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                             <  1:1756784/44‑1 (MQ=255)
                                   gCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                  >  1:945334/1‑71 (MQ=255)
                                    cTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                   <  1:1871319/53‑1 (MQ=255)
                                    cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                 <  1:3369820/71‑1 (MQ=255)
                                    cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                 <  1:807792/71‑1 (MQ=255)
                                    cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                  <  1:1206319/70‑1 (MQ=255)
                                       gACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGACAGCGCAGTCGCCTGACCGTTCTg                              >  1:3455754/1‑71 (MQ=255)
                                        aCTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGt                             <  1:2950033/71‑1 (MQ=255)
                                         ttGGGTCACATCAACGTAGATAGGATCGAgctgct                                                                >  1:1358657/2‑35 (MQ=25)
                                          tGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                           >  1:3263038/1‑71 (MQ=255)
                                             gTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGcc                                                     <  1:719810/42‑1 (MQ=255)
                                               cacaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                      >  1:3279337/1‑71 (MQ=255)
                                                acaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                      <  1:1604765/70‑1 (MQ=255)
                                                acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                   >  1:631459/1‑41 (MQ=255)
                                                acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAGTTCAGTCgtt                                         >  1:3892613/1‑49 (MQ=255)
                                                 caTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcg                    >  1:541414/1‑71 (MQ=255)
                                                 caTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                   <  1:2054337/40‑1 (MQ=255)
                                                  aTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                   >  1:3456739/1‑71 (MQ=255)
                                                   tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                              >  1:167886/1‑43 (MQ=255)
                                                   tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                              >  1:2490898/1‑43 (MQ=255)
                                                   tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                            >  1:1134639/1‑45 (MQ=255)
                                                   tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGcc                  >  1:1457137/1‑71 (MQ=255)
                                                    cAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                     >  1:2738066/1‑67 (MQ=255)
                                                     aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCa                                                    >  1:2041993/1‑35 (MQ=255)
                                                     aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                            <  1:2363394/43‑1 (MQ=255)
                                                     aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                           >  1:3898596/1‑60 (MQ=255)
                                                         tAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg             <  1:3299116/70‑1 (MQ=255)
                                                         tAGATCGGATAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGa                                      >  1:3555581/1‑44 (MQ=38)
                                                          aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt            >  1:1079620/1‑70 (MQ=255)
                                                          aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt            >  1:1906485/1‑70 (MQ=255)
                                                            aTCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCAc         >  1:2066607/1‑71 (MQ=255)
                                                             tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                         <  1:2773043/54‑1 (MQ=255)
                                                             tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACg        <  1:2235131/71‑1 (MQ=255)
                                                               gtaTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt       <  1:2286188/68‑1 (MQ=255)
                                                               ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                         >  1:3383651/1‑52 (MQ=255)
                                                               ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt       <  1:1272330/70‑1 (MQ=255)
                                                                gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                         <  1:1804115/51‑1 (MQ=255)
                                                                gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc     <  1:2933415/71‑1 (MQ=255)
                                                                gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc     <  1:2190978/71‑1 (MQ=255)
                                                                gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc     <  1:2300564/71‑1 (MQ=255)
                                                                gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc     <  1:1210534/71‑1 (MQ=255)
                                                                 aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                         <  1:970748/50‑1 (MQ=255)
                                                                 aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg             <  1:2588989/62‑1 (MQ=255)
                                                                 aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc     >  1:581912/1‑70 (MQ=255)
                                                                  tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                     <  1:1182810/53‑1 (MQ=255)
                                                                  tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa   >  1:2853483/1‑71 (MQ=255)
                                                                  tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa   >  1:629776/1‑71 (MQ=255)
                                                                   cAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                   <  1:1322249/54‑1 (MQ=255)
                                                                    aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAc  <  1:1353681/70‑1 (MQ=255)
                                                                    |                                                                     
TTCGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAC  >  minE/313394‑313531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: