Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 313,465 T→C 7.0% Q208Q (CAA→CAG)  acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE313,4650TC 7.0% 244.1 / 7.4 86Q208Q (CAA→CAG) acrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base T (38/42);  new base C (4/2);  total (42/44)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

CGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTT  >  minE/313396‑313533
                                                                     |                                                                    
cGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGt                                                                      <  1:2771688/70‑1 (MQ=255)
 gCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTTGGTCACATCAACGTAGATCGGATCAAGTtg                                                                    <  1:381997/71‑1 (MQ=255)
     gTTCCTGTTTCAGGAGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGt                                                                      <  1:2785126/65‑1 (MQ=255)
      ttCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                               >  1:2278772/1‑71 (MQ=255)
        ccTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCAc                                                              <  1:450876/70‑1 (MQ=255)
         cTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                            <  1:2646348/71‑1 (MQ=255)
          tGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                    >  1:3620079/1‑62 (MQ=255)
          tGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                    >  1:2638386/1‑62 (MQ=255)
           gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                          >  1:2545727/1‑71 (MQ=255)
           gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                          >  1:1695547/1‑71 (MQ=255)
            tttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTg                                                         >  1:3390775/1‑71 (MQ=255)
             ttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGc                                                        >  1:975161/1‑71 (MQ=255)
              tCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtgctg                                                                 >  1:2865771/1‑61 (MQ=255)
               cAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTATATCGGATCAAGTTGCTGCACgg                                                            <  1:1447814/65‑1 (MQ=255)
                aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                     <  1:3427590/71‑1 (MQ=255)
                aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                     <  1:3770735/71‑1 (MQ=255)
                aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                     >  1:423130/1‑71 (MQ=255)
                    gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                 <  1:953390/71‑1 (MQ=255)
                    gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                 <  1:889669/71‑1 (MQ=255)
                     cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                 <  1:2702468/70‑1 (MQ=255)
                     cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                <  1:358370/71‑1 (MQ=255)
                      aGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                           <  1:2928788/59‑1 (MQ=255)
                      aGGAAGTCGTTGCTGGACT‑GGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                           <  1:610422/58‑1 (MQ=255)
                       ggAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                              <  1:1496254/71‑1 (MQ=255)
                         aaGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGc                                            <  1:3402261/71‑1 (MQ=255)
                          aGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                    >  1:3157123/1‑46 (MQ=255)
                            tCGTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGcc                                                       >  1:1482115/1‑57 (MQ=255)
                            tCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTtg                                                                    <  1:3384712/44‑1 (MQ=255)
                              gTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAgttc                                                  >  1:3406562/1‑57 (MQ=255)
                              gTTGCTGGACTGGGTCATATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                            <  1:1763547/50‑1 (MQ=255)
                              gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                            <  1:3638478/50‑1 (MQ=255)
                              gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTg                                         >  1:411650/1‑69 (MQ=255)
                              gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGAc                                       >  1:788561/1‑71 (MQ=255)
                               ttGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                          >  1:2392360/1‑51 (MQ=255)
                                tGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                               >  1:469065/1‑45 (MQ=255)
                                tGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                          >  1:3839957/1‑50 (MQ=255)
                                 gCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCa                                                               <  1:1756784/44‑1 (MQ=255)
                                 gCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                    >  1:945334/1‑71 (MQ=255)
                                  cTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                     <  1:1871319/53‑1 (MQ=255)
                                  cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                   <  1:3369820/71‑1 (MQ=255)
                                  cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                   <  1:807792/71‑1 (MQ=255)
                                  cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                    <  1:1206319/70‑1 (MQ=255)
                                     gACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGACAGCGCAGTCGCCTGACCGTTCTg                                >  1:3455754/1‑71 (MQ=255)
                                      aCTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGt                               <  1:2950033/71‑1 (MQ=255)
                                       ttGGGTCACATCAACGTAGATAGGATCGAgctgct                                                                  >  1:1358657/2‑35 (MQ=25)
                                        tGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                             >  1:3263038/1‑71 (MQ=255)
                                           gTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGcc                                                       <  1:719810/42‑1 (MQ=255)
                                             cacaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                        >  1:3279337/1‑71 (MQ=255)
                                              acaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                        <  1:1604765/70‑1 (MQ=255)
                                              acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                     >  1:631459/1‑41 (MQ=255)
                                              acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAGTTCAGTCgtt                                           >  1:3892613/1‑49 (MQ=255)
                                               caTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcg                      >  1:541414/1‑71 (MQ=255)
                                               caTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                     <  1:2054337/40‑1 (MQ=255)
                                                aTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                     >  1:3456739/1‑71 (MQ=255)
                                                 tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                >  1:2490898/1‑43 (MQ=255)
                                                 tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                >  1:167886/1‑43 (MQ=255)
                                                 tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                              >  1:1134639/1‑45 (MQ=255)
                                                 tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGcc                    >  1:1457137/1‑71 (MQ=255)
                                                  cAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                       >  1:2738066/1‑67 (MQ=255)
                                                   aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCa                                                      >  1:2041993/1‑35 (MQ=255)
                                                   aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                              <  1:2363394/43‑1 (MQ=255)
                                                   aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                             >  1:3898596/1‑60 (MQ=255)
                                                       tAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg               <  1:3299116/70‑1 (MQ=255)
                                                       tAGATCGGAT‑AAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGa                                        >  1:3555581/1‑44 (MQ=38)
                                                        aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt              >  1:1079620/1‑70 (MQ=255)
                                                        aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt              >  1:1906485/1‑70 (MQ=255)
                                                          aTCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCAc           >  1:2066607/1‑71 (MQ=255)
                                                           tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                           <  1:2773043/54‑1 (MQ=255)
                                                           tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACg          <  1:2235131/71‑1 (MQ=255)
                                                             gtaTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt         <  1:2286188/68‑1 (MQ=255)
                                                             ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                           >  1:3383651/1‑52 (MQ=255)
                                                             ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt         <  1:1272330/70‑1 (MQ=255)
                                                              gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                           <  1:1804115/51‑1 (MQ=255)
                                                              gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc       <  1:2300564/71‑1 (MQ=255)
                                                              gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc       <  1:2190978/71‑1 (MQ=255)
                                                              gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc       <  1:1210534/71‑1 (MQ=255)
                                                              gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc       <  1:2933415/71‑1 (MQ=255)
                                                               aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                           <  1:970748/50‑1 (MQ=255)
                                                               aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg               <  1:2588989/62‑1 (MQ=255)
                                                               aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc       >  1:581912/1‑70 (MQ=255)
                                                                tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                       <  1:1182810/53‑1 (MQ=255)
                                                                tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa     >  1:629776/1‑71 (MQ=255)
                                                                tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa     >  1:2853483/1‑71 (MQ=255)
                                                                 cAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                     <  1:1322249/54‑1 (MQ=255)
                                                                  aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAc    <  1:1353681/70‑1 (MQ=255)
                                                                   aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGcc                    >  1:3416875/1‑53 (MQ=255)
                                                                   aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACtt  <  1:158687/71‑1 (MQ=255)
                                                                   aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACtt  <  1:2862113/71‑1 (MQ=255)
                                                                   aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt   >  1:2719315/1‑70 (MQ=255)
                                                                     |                                                                    
CGCCAGTTCCTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTT  >  minE/313396‑313533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑