Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	313,474	G→A	7.5%	T205T (ACC→ACT)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	313,474	0	G	A	7.5%	242.1 / 7.0	80	T205T (ACC→ACT)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base G (35/39);  new base A (4/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAC  >  minE/313405‑313544                                                                      |                                                                       cTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                                       <  1:2646348/71‑1 (MQ=255)   gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                                     >  1:2545727/1‑71 (MQ=255)   gTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                                     >  1:1695547/1‑71 (MQ=255)    tttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTg                                                                    >  1:3390775/1‑71 (MQ=255)     ttCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGc                                                                   >  1:975161/1‑71 (MQ=255)       cAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTATATCGGATCAAGTTGCTGCACgg                                                                       <  1:1447814/65‑1 (MQ=255)        aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                                <  1:3427590/71‑1 (MQ=255)        aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                                <  1:3770735/71‑1 (MQ=255)        aGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAg                                                                >  1:423130/1‑71 (MQ=255)            gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                            <  1:953390/71‑1 (MQ=255)            gcAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                            <  1:889669/71‑1 (MQ=255)             cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCa                                                            <  1:2702468/70‑1 (MQ=255)             cAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                           <  1:358370/71‑1 (MQ=255)              aGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                                      <  1:2928788/59‑1 (MQ=255)              aGGAAGTCGTTGCTGGACT‑GGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGt                                                                      <  1:610422/58‑1 (MQ=255)               ggAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                                         <  1:1496254/71‑1 (MQ=255)                 aaGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGc                                                       <  1:3402261/71‑1 (MQ=255)                    tCGTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGcc                                                                  >  1:1482115/1‑57 (MQ=255)                      gTTGCTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAgttc                                                             >  1:3406562/1‑57 (MQ=255)                      gTTGCTGGACTGGGTCATATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                                       <  1:1763547/50‑1 (MQ=255)                      gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACgg                                                                       <  1:3638478/50‑1 (MQ=255)                      gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTg                                                    >  1:411650/1‑69 (MQ=255)                      gTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGAc                                                  >  1:788561/1‑71 (MQ=255)                       ttGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                                     >  1:2392360/1‑51 (MQ=255)                        tGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGtt                                                                     >  1:3839957/1‑50 (MQ=255)                         gCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                               >  1:945334/1‑71 (MQ=255)                          cTGGATTGGGTCACATCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                                <  1:1871319/53‑1 (MQ=255)                          cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                              <  1:807792/71‑1 (MQ=255)                          cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGtt                                              <  1:3369820/71‑1 (MQ=255)                          cTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGt                                               <  1:1206319/70‑1 (MQ=255)                             gACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGACAGCGCAGTCGCCTGACCGTTCTg                                           >  1:3455754/1‑71 (MQ=255)                              aCTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGt                                          <  1:2950033/71‑1 (MQ=255)                                tGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                                        >  1:3263038/1‑71 (MQ=255)                                   gTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGcc                                                                  <  1:719810/42‑1 (MQ=255)                                     cacaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                                   >  1:3279337/1‑71 (MQ=255)                                      acaTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATg                                   <  1:1604765/70‑1 (MQ=255)                                      acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                                >  1:631459/1‑41 (MQ=255)                                      acaTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAGTTCAGTCgtt                                                      >  1:3892613/1‑49 (MQ=255)                                       caTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcg                                 >  1:541414/1‑71 (MQ=255)                                       caTCAACGTAGATAGGATCGAGCTGCTGGACAGTCGCCAg                                                                <  1:2054337/40‑1 (MQ=255)                                        aTCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                                >  1:3456739/1‑71 (MQ=255)                                         tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                           >  1:2490898/1‑43 (MQ=255)                                         tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAg                                                           >  1:167886/1‑43 (MQ=255)                                         tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                                         >  1:1134639/1‑45 (MQ=255)                                         tCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGcc                               >  1:1457137/1‑71 (MQ=255)                                          cAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                                  >  1:2738066/1‑67 (MQ=255)                                           aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCa                                                                 >  1:2041993/1‑35 (MQ=255)                                           aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTc                                                         <  1:2363394/43‑1 (MQ=255)                                           aaCGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTAc                                        >  1:3898596/1‑60 (MQ=255)                                               tAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg                          <  1:3299116/70‑1 (MQ=255)                                               tAGATCGGAT‑AAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGa                                                   >  1:3555581/1‑44 (MQ=38)                                                aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt                         >  1:1906485/1‑70 (MQ=255)                                                aGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGt                         >  1:1079620/1‑70 (MQ=255)                                                  aTCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCAc                      >  1:2066607/1‑71 (MQ=255)                                                   tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                                      <  1:2773043/54‑1 (MQ=255)                                                   tCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACg                     <  1:2235131/71‑1 (MQ=255)                                                     gtaTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt                    <  1:2286188/68‑1 (MQ=255)                                                     ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                                      >  1:3383651/1‑52 (MQ=255)                                                     ggATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGt                    <  1:1272330/70‑1 (MQ=255)                                                      gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                                      <  1:1804115/51‑1 (MQ=255)                                                      gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                  <  1:2300564/71‑1 (MQ=255)                                                      gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                  <  1:2933415/71‑1 (MQ=255)                                                      gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                  <  1:2190978/71‑1 (MQ=255)                                                      gATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                  <  1:1210534/71‑1 (MQ=255)                                                       aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCa                                      <  1:970748/50‑1 (MQ=255)                                                       aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCg                          <  1:2588989/62‑1 (MQ=255)                                                       aTCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTc                  >  1:581912/1‑70 (MQ=255)                                                        tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgc                                  <  1:1182810/53‑1 (MQ=255)                                                        tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                >  1:2853483/1‑71 (MQ=255)                                                        tCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGa                >  1:629776/1‑71 (MQ=255)                                                         cAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATgcgc                                <  1:1322249/54‑1 (MQ=255)                                                          aaGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGAc               <  1:1353681/70‑1 (MQ=255)                                                           aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGcc                               >  1:3416875/1‑53 (MQ=255)                                                           aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACtt             <  1:2862113/71‑1 (MQ=255)                                                           aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACtt             <  1:158687/71‑1 (MQ=255)                                                           aGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt              >  1:2719315/1‑70 (MQ=255)                                                               gctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTAcc          >  1:678621/1‑70 (MQ=255)                                                                ctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCa         <  1:289564/70‑1 (MQ=255)                                                                  gcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACt              <  1:1958062/63‑1 (MQ=255)                                                                   cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGtt                   >  1:1468650/1‑57 (MQ=255)                                                                   cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc     <  1:690335/71‑1 (MQ=255)                                                                    aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc     >  1:119929/1‑70 (MQ=255)                                                                     cGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCg    <  1:3711216/70‑1 (MQ=255)                                                                     cGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa   <  1:1682029/71‑1 (MQ=255)                                                                      ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTcc                           <  1:144312/46‑1 (MQ=255)                                                                      ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc  >  1:80691/1‑71 (MQ=255)                                                                      |                                                                       CTGTTTCAGGCGCAGGAAGTCGTTGCTGGACTGGGTCACATCAACGTAGATCGGATCAAGTTGCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAC  >  minE/313405‑313544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑