Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,232	C→G	11.3%	L175L (CTC→CTG)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,232	0	C	G	11.3%	213.6 / 15.1	80	L175L (CTC→CTG)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base C (41/29);  new base G (5/4);  total (47/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

CTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAT  >  minE/132162‑132300                                                                       |                                                                     cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                      <  1:1654283/71‑1 (MQ=255) cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                                      >  1:1154686/1‑71 (MQ=255)    tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                     >  1:3672365/1‑69 (MQ=255)     cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                     <  1:1911822/68‑1 (MQ=255)       cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                                >  1:1559944/1‑71 (MQ=255)        gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                               >  1:3282126/1‑71 (MQ=255)          tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                                   >  1:2689060/1‑65 (MQ=255)          tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                             >  1:3699296/1‑71 (MQ=255)          tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                             >  1:1378704/1‑71 (MQ=255)               gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                         <  1:2961241/70‑1 (MQ=255)                  atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                     >  1:524861/1‑71 (MQ=255)                  atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                     >  1:312576/1‑71 (MQ=255)                  atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                         <  1:232113/67‑1 (MQ=255)                   tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                     >  1:3279009/1‑70 (MQ=255)                   tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                     >  1:1589625/1‑70 (MQ=255)                    cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                     <  1:820672/53‑1 (MQ=255)                     aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                  <  1:3378901/71‑1 (MQ=255)                     aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTAGAact                                                                 >  1:2996407/1‑56 (MQ=255)                     aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                  <  1:3916233/71‑1 (MQ=255)                     aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                  <  1:1866093/71‑1 (MQ=255)                      ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                        >  1:501805/1‑64 (MQ=255)                      ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                  >  1:369444/1‑70 (MQ=255)                      ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                  <  1:895428/70‑1 (MQ=255)                      ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                                 >  1:3018776/1‑71 (MQ=255)                       cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                >  1:3459369/1‑71 (MQ=255)                       cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                >  1:1094608/1‑71 (MQ=255)                       aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                >  1:2295944/2‑71 (MQ=255)                        aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                >  1:1704122/1‑70 (MQ=255)                         tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                                     <  1:3474398/48‑1 (MQ=255)                         tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                   >  1:413424/1‑66 (MQ=255)                         tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                     <  1:3129806/64‑1 (MQ=255)                         tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                               >  1:2795204/1‑70 (MQ=255)                         tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                              <  1:822055/71‑1 (MQ=255)                          cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                              <  1:353302/67‑1 (MQ=255)                          cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                   >  1:1445872/1‑65 (MQ=255)                           acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                                   >  1:1816522/1‑48 (MQ=255)                           acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                              >  1:938210/1‑69 (MQ=255)                           acacCGGATGACGAAATCATGCAACATCGGCGGATAGCGATTCTCg                                                                     >  1:538351/1‑46 (MQ=255)                            cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                             <  1:1085283/69‑1 (MQ=255)                             acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                              <  1:2242153/51‑1 (MQ=255)                             acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                          >  1:3892751/1‑71 (MQ=255)                             acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                          >  1:1215807/1‑71 (MQ=255)                                ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                       <  1:1739920/71‑1 (MQ=255)                                   tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                          >  1:3322513/1‑65 (MQ=255)                                    gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                         <  1:2582517/49‑1 (MQ=255)                                    gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                         <  1:368264/49‑1 (MQ=255)                                      cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                              <  1:3826494/58‑1 (MQ=255)                                      cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                              <  1:2036055/58‑1 (MQ=255)                                       gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                  <  1:2685332/66‑1 (MQ=255)                                         aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                      <  1:3338305/47‑1 (MQ=255)                                         aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                              >  1:74025/1‑71 (MQ=255)                                          aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                        <  1:3299476/44‑1 (MQ=255)                                          aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                      >  1:2171812/1‑46 (MQ=255)                                           tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                            >  1:477006/1‑71 (MQ=255)                                           tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                            <  1:3405915/71‑1 (MQ=255)                                            cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                           >  1:643085/1‑71 (MQ=255)                                              tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                       >  1:2239041/1‑41 (MQ=255)                                               gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                 >  1:1559300/1‑62 (MQ=255)                                               gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                        >  1:664640/1‑71 (MQ=255)                                                cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                       <  1:1122052/71‑1 (MQ=255)                                                 aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                      <  1:1854168/71‑1 (MQ=255)                                                    aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                   >  1:1491341/1‑71 (MQ=255)                                                     tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                   >  1:507433/1‑70 (MQ=255)                                                     tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                  <  1:589586/71‑1 (MQ=255)                                                     tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                  <  1:3760758/71‑1 (MQ=255)                                                        gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc               <  1:1494823/71‑1 (MQ=255)                                                         cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt              >  1:1326631/1‑71 (MQ=255)                                                          ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg             >  1:3369448/1‑71 (MQ=255)                                                          ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg             >  1:2282189/1‑71 (MQ=255)                                                            aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                          <  1:2972567/40‑1 (MQ=255)                                                            aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt           >  1:290260/1‑71 (MQ=255)                                                              tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                              >  1:3139451/1‑50 (MQ=255)                                                              tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa         >  1:3660091/1‑71 (MQ=255)                                                              tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa         >  1:2096639/1‑71 (MQ=255)                                                                cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                            >  1:3594428/1‑50 (MQ=255)                                                                cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc        <  1:1619129/70‑1 (MQ=255)                                                                cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc        <  1:1040486/70‑1 (MQ=255)                                                                cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg       >  1:2472892/1‑71 (MQ=255)                                                                  aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                       <  1:2496046/53‑1 (MQ=255)                                                                   ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                        <  1:66698/51‑1 (MQ=255)                                                                   ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt     >  1:960573/1‑70 (MQ=255)                                                                   ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt     >  1:2838467/1‑70 (MQ=255)                                                                    tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                   <  1:3322401/39‑1 (MQ=255)                                                                     ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa   <  1:721548/70‑1 (MQ=255)                                                                     ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa   <  1:1305193/70‑1 (MQ=255)                                                                     ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt  >  1:110300/1‑71 (MQ=255)                                                                      tcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                 >  1:545898/1‑39 (MQ=255)                                                                       |                                                                     CTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAT  >  minE/132162‑132300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑