| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 337,128 | G→T | 20.8% | intergenic (+100/+216) | ybaQ → / ← copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 337,128 | 0 | G | T | 20.8% | 185.1 / 23.5 | 67 | intergenic (+100/+216) | ybaQ/copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Reads supporting (aligned to +/- strand): ref base G (26/27); new base T (4/10); total (30/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
GGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAC > minE/337064‑337195 | ggCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTAc < 1:2405529/71‑1 (MQ=39) gCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACa < 1:3729263/71‑1 (MQ=39) gCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGAAGCGACGCTTACCCGTCTTATCAGGCCTACa < 1:1890017/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACaa < 1:3936250/71‑1 (MQ=39) cACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACt < 1:2951325/70‑1 (MQ=39) cACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACt < 1:2942945/70‑1 (MQ=39) aCGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACa < 1:3926497/65‑1 (MQ=35) cgccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACtt < 1:3781080/69‑1 (MQ=39) cgccgcATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:3214427/71‑1 (MQ=39) gcatccggcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCt > 1:1871703/1‑71 (MQ=39) ccggcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAAc > 1:3723918/1‑71 (MQ=39) cggcatccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAAc < 1:2398076/70‑1 (MQ=40) catccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTAc > 1:2806241/1‑51 (MQ=35) catccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTa < 1:2670650/71‑1 (MQ=39) atccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAg < 1:3467092/71‑1 (MQ=39) tccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAgg < 1:1714184/71‑1 (MQ=255) ccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCt > 1:2401576/1‑60 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:2090379/1‑71 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:2095066/1‑71 (MQ=255) gAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:1588869/54‑1 (MQ=255) aGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt < 1:2421216/53‑1 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa > 1:1527728/1‑71 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1668501/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGtata < 1:1462145/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATa < 1:2735455/71‑1 (MQ=255) cATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAg > 1:972171/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:2047034/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:922520/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:1698064/1‑71 (MQ=255) tGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCg > 1:2394883/1‑71 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2412190/1‑71 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2434305/1‑71 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATaa > 1:3215591/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATaa > 1:2849537/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:1626123/71‑1 (MQ=39) tGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:3633491/1‑71 (MQ=39) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2083478/1‑67 (MQ=255) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc > 1:862347/1‑71 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTtgt > 1:3543244/1‑39 (MQ=255) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcg < 1:3194471/71‑1 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc < 1:2836967/70‑1 (MQ=40) cGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1553886/56‑1 (MQ=255) aCGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgca < 1:2175251/71‑1 (MQ=39) ttACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatc > 1:644828/1‑69 (MQ=40) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2559889/67‑1 (MQ=11) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2135614/67‑1 (MQ=11) aCCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:1864280/1‑70 (MQ=40) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:3312398/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:2193354/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatc > 1:2263847/2‑65 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCgg > 1:108496/2‑44 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgcatccggcag > 1:3188136/2‑70 (MQ=11) gTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcagtt < 1:2350782/71‑4 (MQ=11) cTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccggcatcc > 1:75634/1‑69 (MQ=40) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:3246850/49‑1 (MQ=11) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:3485050/49‑1 (MQ=11) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:2229522/49‑1 (MQ=11) ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg > 1:245724/1‑71 (MQ=255) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccgc < 1:2620764/56‑1 (MQ=11) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatcc < 1:2685157/60‑1 (MQ=11) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:1594343/68‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg < 1:814902/69‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:3421221/1‑71 (MQ=255) tCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcat > 1:3670952/1‑56 (MQ=11) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:2008335/67‑1 (MQ=255) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa < 1:1098202/70‑1 (MQ=255) cAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAc > 1:3127716/1‑70 (MQ=255) | GGCGTTCACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAC > minE/337064‑337195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |