Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	132,238	A→G	11.8%	L177L (CTA→CTG)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	132,238	0	A	G	11.8%	200.7 / 15.6	76	L177L (CTA→CTG)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base A (38/29);  new base G (5/4);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACG  >  minE/132168‑132305                                                                       |                                                                    cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                                     >  1:1559944/1‑71 (MQ=255)  gCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactac                                                                    >  1:3282126/1‑71 (MQ=255)    tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                  >  1:1378704/1‑71 (MQ=255)    tggtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTg                                                                  >  1:3699296/1‑71 (MQ=255)         gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                              <  1:2961241/70‑1 (MQ=255)            atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                          >  1:312576/1‑71 (MQ=255)            atCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                          >  1:524861/1‑71 (MQ=255)            atCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                              <  1:232113/67‑1 (MQ=255)             tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                          >  1:3279009/1‑70 (MQ=255)             tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                          >  1:1589625/1‑70 (MQ=255)               aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                       <  1:3378901/71‑1 (MQ=255)               aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                       <  1:3916233/71‑1 (MQ=255)               aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                       <  1:1866093/71‑1 (MQ=255)                ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCaaaa                                                             >  1:501805/1‑64 (MQ=255)                ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                       <  1:895428/70‑1 (MQ=255)                ccATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCatat                                                       >  1:369444/1‑70 (MQ=255)                ccATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATAtt                                                      >  1:3018776/1‑71 (MQ=255)                 cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                     >  1:1094608/1‑71 (MQ=255)                 cATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                     >  1:3459369/1‑71 (MQ=255)                 aaTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                     >  1:2295944/2‑71 (MQ=255)                  aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTc                                                     >  1:1704122/1‑70 (MQ=255)                   tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                        >  1:413424/1‑66 (MQ=255)                   tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                          <  1:3129806/64‑1 (MQ=255)                   tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                    >  1:2795204/1‑70 (MQ=255)                   tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                   <  1:822055/71‑1 (MQ=255)                    cgtacCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGc                                                   <  1:353302/67‑1 (MQ=255)                    cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                        >  1:1445872/1‑65 (MQ=255)                     acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                   >  1:938210/1‑69 (MQ=255)                      cacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                  <  1:1085283/69‑1 (MQ=255)                       acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAactact                                                                   <  1:2242153/51‑1 (MQ=255)                       acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                               >  1:1215807/1‑71 (MQ=255)                       acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                               >  1:3892751/1‑71 (MQ=255)                          ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGcgcg                                            <  1:1739920/71‑1 (MQ=255)                             tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                               >  1:3322513/1‑65 (MQ=255)                              gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                              <  1:2582517/49‑1 (MQ=255)                              gACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                              <  1:368264/49‑1 (MQ=255)                                cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                   <  1:2036055/58‑1 (MQ=255)                                cGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                   <  1:3826494/58‑1 (MQ=255)                                 gagaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                       <  1:2685332/66‑1 (MQ=255)                                   aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                           <  1:3338305/47‑1 (MQ=255)                                   aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                   >  1:74025/1‑71 (MQ=255)                                    aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                             <  1:3299476/44‑1 (MQ=255)                                    aTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAAc                                                           >  1:2171812/1‑46 (MQ=255)                                     tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                 >  1:477006/1‑71 (MQ=255)                                     tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                 <  1:3405915/71‑1 (MQ=255)                                      cATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                >  1:643085/1‑71 (MQ=255)                                        tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                            >  1:2239041/1‑41 (MQ=255)                                         gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                      >  1:1559300/1‑62 (MQ=255)                                         gCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                             >  1:664640/1‑71 (MQ=255)                                          cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCACCGCGACTTAATGTTATTGCt                            <  1:1122052/71‑1 (MQ=255)                                           aaCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                           <  1:1854168/71‑1 (MQ=255)                                              aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                        >  1:1491341/1‑71 (MQ=255)                                               tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                        >  1:507433/1‑70 (MQ=255)                                               tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                       <  1:589586/71‑1 (MQ=255)                                               tCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                       <  1:3760758/71‑1 (MQ=255)                                                  gcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                    <  1:1494823/71‑1 (MQ=255)                                                   cggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                   >  1:1326631/1‑71 (MQ=255)                                                    ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                  >  1:2282189/1‑71 (MQ=255)                                                    ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTg                  >  1:3369448/1‑71 (MQ=255)                                                      aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgc                                               <  1:2972567/40‑1 (MQ=255)                                                      aTTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                >  1:290260/1‑71 (MQ=255)                                                        tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                   >  1:3139451/1‑50 (MQ=255)                                                        tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa              >  1:2096639/1‑71 (MQ=255)                                                        tGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCa              >  1:3660091/1‑71 (MQ=255)                                                          cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                 >  1:3594428/1‑50 (MQ=255)                                                          cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc             <  1:1619129/70‑1 (MQ=255)                                                          cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCAc             <  1:1040486/70‑1 (MQ=255)                                                          cGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACg            >  1:2472892/1‑71 (MQ=255)                                                            aTTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                            <  1:2496046/53‑1 (MQ=255)                                                             ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGc                             <  1:66698/51‑1 (MQ=255)                                                             ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt          >  1:960573/1‑70 (MQ=255)                                                             ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt          >  1:2838467/1‑70 (MQ=255)                                                              tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                        <  1:3322401/39‑1 (MQ=255)                                                               ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa        <  1:721548/70‑1 (MQ=255)                                                               ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa        <  1:1305193/70‑1 (MQ=255)                                                               ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt       >  1:110300/1‑71 (MQ=255)                                                                tcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                      >  1:545898/1‑39 (MQ=255)                                                                  gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                           >  1:1969613/1‑48 (MQ=255)                                                                  gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga    <  1:2385048/71‑1 (MQ=255)                                                                    actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacg  <  1:3109764/71‑1 (MQ=255)                                                                      tactGCAAAAACATATTCGCCAGCGCGACTTAATGTTa                                 >  1:3852576/1‑38 (MQ=255)                                                                       |                                                                    CGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACG  >  minE/132168‑132305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑