| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 337,151 | C→G | 25.8% | intergenic (+123/+193) | ybaQ → / ← copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 337,151 | 0 | C | G | 25.8% | 135.2 / 41.2 | 66 | intergenic (+123/+193) | ybaQ/copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Reads supporting (aligned to +/- strand): ref base C (29/20); new base G (6/11); total (35/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
CATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGT > minE/337084‑337221 | catccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTa < 1:2670650/71‑1 (MQ=39) atccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAg < 1:3467092/71‑1 (MQ=39) tccgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAgg < 1:1714184/71‑1 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:2090379/1‑71 (MQ=255) cgAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGcc > 1:2095066/1‑71 (MQ=255) gCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGa > 1:1527728/1‑71 (MQ=255) cACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1668501/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGtata < 1:1462145/71‑1 (MQ=255) aCCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATa < 1:2735455/71‑1 (MQ=255) cATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAg > 1:972171/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:922520/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:2047034/1‑71 (MQ=255) ttGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGc > 1:1698064/1‑71 (MQ=255) tGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCg > 1:2394883/1‑71 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2412190/1‑71 (MQ=255) gCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2434305/1‑71 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATaa > 1:3215591/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATaa > 1:2849537/1‑65 (MQ=255) ccTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:1626123/71‑1 (MQ=39) tGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:3633491/1‑71 (MQ=39) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGt > 1:2083478/1‑67 (MQ=255) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc > 1:862347/1‑71 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcg < 1:3194471/71‑1 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc < 1:2836967/70‑1 (MQ=40) cGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGAt < 1:1553886/56‑1 (MQ=255) aCGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgca < 1:2175251/71‑1 (MQ=39) ttACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatc > 1:644828/1‑69 (MQ=40) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2135614/67‑1 (MQ=11) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2559889/67‑1 (MQ=11) aCCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:1864280/1‑70 (MQ=40) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:2193354/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:3312398/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatc > 1:2263847/2‑65 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCgg > 1:108496/2‑44 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgcatccggcag > 1:3188136/2‑70 (MQ=11) gTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcagtt < 1:2350782/71‑4 (MQ=11) cTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccggcatcc > 1:75634/1‑69 (MQ=40) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:3485050/49‑1 (MQ=11) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:2229522/49‑1 (MQ=11) ttATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGtt < 1:3246850/49‑1 (MQ=11) ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg > 1:245724/1‑71 (MQ=255) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccgc < 1:2620764/56‑1 (MQ=11) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatcc < 1:2685157/60‑1 (MQ=11) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:1594343/68‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg < 1:814902/69‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:3421221/1‑71 (MQ=255) tCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcat > 1:3670952/1‑56 (MQ=11) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:2008335/67‑1 (MQ=255) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa < 1:1098202/70‑1 (MQ=255) cAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAc > 1:3127716/1‑70 (MQ=255) atgCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCg < 1:1631977/40‑1 (MQ=14) ccTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTg < 1:70359/71‑1 (MQ=255) tACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGtt < 1:1105033/39‑1 (MQ=40) aCAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCTAg > 1:491038/1‑60 (MQ=255) aCAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAcc < 1:783144/64‑1 (MQ=255) cAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag > 1:2898298/1‑53 (MQ=11) cAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag > 1:1196675/1‑53 (MQ=11) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:1801825/1‑39 (MQ=40) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccggca < 1:1610931/53‑1 (MQ=35) aaaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc > 1:3417190/1‑39 (MQ=40) aaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt > 1:115674/1‑71 (MQ=255) aaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt > 1:999915/1‑71 (MQ=255) gtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGAc > 1:633180/1‑71 (MQ=255) gtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGAc > 1:2354274/1‑71 (MQ=255) gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt < 1:2182144/60‑1 (MQ=255) aCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTAcgc > 1:2557321/1‑71 (MQ=40) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGt < 1:1431032/71‑1 (MQ=39) | CATCCGAGCACCATTGCCTGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGT > minE/337084‑337221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |