| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 337,171 | T→C | 21.6% | intergenic (+143/+173) | ybaQ → / ← copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 337,171 | 0 | T | C | 21.6% | 114.4 / 23.6 | 51 | intergenic (+143/+173) | ybaQ/copA | predicted DNA‑binding transcriptional regulator/copper transporter |
| Reads supporting (aligned to +/- strand): ref base T (24/16); new base C (4/7); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACC > minE/337102‑337240 | tGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:3633491/1‑71 (MQ=39) gATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc > 1:862347/1‑71 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAcg < 1:3194471/71‑1 (MQ=39) aTGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc < 1:2836967/70‑1 (MQ=40) aCGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgca < 1:2175251/71‑1 (MQ=39) ttACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatc > 1:644828/1‑69 (MQ=40) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2559889/67‑1 (MQ=11) gcgcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccgg < 1:2135614/67‑1 (MQ=11) aCCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccgg > 1:1864280/1‑70 (MQ=40) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:3312398/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag < 1:2193354/70‑2 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatc > 1:2263847/2‑65 (MQ=11) gcGTCTTATCATGCCTACAAACTTGTGCCGGATCGGTAGGCCGGATAAGGCGTTTATGCCgcatccggcag > 1:3188136/2‑70 (MQ=11) gTCTTATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcagtt < 1:2350782/71‑4 (MQ=11) cTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccggcatcc > 1:75634/1‑69 (MQ=40) ttATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg > 1:245724/1‑71 (MQ=255) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCAcgccgc < 1:2620764/56‑1 (MQ=11) tATCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatcc < 1:2685157/60‑1 (MQ=11) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:1594343/68‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAg < 1:814902/69‑1 (MQ=255) aTCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa > 1:3421221/1‑71 (MQ=255) tCATGCCTACAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcat > 1:3670952/1‑56 (MQ=11) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGa < 1:2008335/67‑1 (MQ=255) tCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCa < 1:1098202/70‑1 (MQ=255) cAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAc > 1:3127716/1‑70 (MQ=255) ccTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTg < 1:70359/71‑1 (MQ=255) aCAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCTAg > 1:491038/1‑60 (MQ=255) aCAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCAcc < 1:783144/64‑1 (MQ=255) cAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag > 1:2898298/1‑53 (MQ=11) cAAACTTGTGCCTGAACGGTAGGACGGATAAGGCGTTCACGCCgcatccggcag > 1:1196675/1‑53 (MQ=11) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTa > 1:1801825/1‑39 (MQ=40) cAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCgcatccggca < 1:1610931/53‑1 (MQ=35) aaaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTAc > 1:3417190/1‑39 (MQ=40) aaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt > 1:999915/1‑71 (MQ=255) aaCTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt > 1:115674/1‑71 (MQ=255) gtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGAc > 1:2354274/1‑71 (MQ=255) gtgCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGAc > 1:633180/1‑71 (MQ=255) gAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGAt < 1:2182144/60‑1 (MQ=255) aCCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTAcgc > 1:2557321/1‑71 (MQ=40) cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGt < 1:1431032/71‑1 (MQ=39) tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCt > 1:3941135/1‑71 (MQ=39) ggCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTa > 1:3660796/1‑71 (MQ=39) ggCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTa < 1:3248760/71‑1 (MQ=39) ggCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTa < 1:1966153/71‑1 (MQ=39) cGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTAt > 1:785950/1‑69 (MQ=40) ggATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAg > 1:3810200/1‑71 (MQ=39) ggATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAg < 1:1935317/71‑1 (MQ=39) gATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGt < 1:503095/62‑1 (MQ=40) gATAAGGCGTTTAAGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAgg > 1:2663028/1‑71 (MQ=255) aaGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCt > 1:3910981/1‑41 (MQ=38) cGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACa > 1:3522680/1‑70 (MQ=40) ttACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAAcc > 1:1558527/1‑71 (MQ=39) tACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc > 1:1275691/1‑61 (MQ=37) tACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGc > 1:2809601/1‑61 (MQ=37) | TGATGCGACGCTTACCCGTCTTATCAGGCCTACAAACTTGTGCCTGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATCCGAGCACCATTGCCTGATGCGACGCTTACGCGTCTTATCAGGCCTACAAACC > minE/337102‑337240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |