| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 349,960 | C→T | 56.4% | P155L (CCC→CTC) | hyi → | hydroxypyruvate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 349,960 | 0 | C | T | 56.4% | 17.6 / 65.9 | 55 | P155L (CCC→CTC) | hyi | hydroxypyruvate isomerase |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base T (13/18); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGA > minE/349893‑350029 | cTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCa < 1:1748389/70‑1 (MQ=255) gaAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGaa > 1:2860933/1‑71 (MQ=255) aagaagATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACt < 1:1309045/71‑1 (MQ=255) aagaagATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACt > 1:24312/1‑71 (MQ=255) aagaagATATTTTATTACTGATTGAACCTATTAACCATTTTGAAATTCCTGGTTTCCATCCCACCGGAACt < 1:2973174/71‑1 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:568899/71‑1 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGc < 1:3818376/71‑1 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCgg > 1:2274141/1‑70 (MQ=255) agaTATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGc < 1:102913/71‑1 (MQ=255) ttttATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACt < 1:2322704/62‑1 (MQ=255) ttttATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACt < 1:236470/62‑1 (MQ=255) ttattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcgc < 1:3474991/70‑1 (MQ=255) tattaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCt > 1:2047750/1‑70 (MQ=255) ttaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGa < 1:1840933/70‑1 (MQ=255) ttaCTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGa < 1:1174318/70‑1 (MQ=255) tGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCa > 1:2404434/1‑58 (MQ=255) tGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCa > 1:657771/1‑58 (MQ=255) aTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattgat > 1:2519822/1‑71 (MQ=255) aTTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattg < 1:2928148/69‑1 (MQ=255) aTTGAACCTATTAACCATTTTGATATTCCTGGGTTCCATCTCACCGGAACTCGGCAGGCGCTGAAattg < 1:3450762/69‑1 (MQ=255) tGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGa > 1:1921451/1‑62 (MQ=255) ccTATTAACCATTTTGATATTCCTGGTTTCCATCTCAc < 1:3730607/38‑1 (MQ=255) ccTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatga < 1:1230040/71‑1 (MQ=255) aTTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCt < 1:1960218/53‑1 (MQ=255) ttAACCATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATtgatg < 1:1647709/66‑1 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttgg > 1:2126811/1‑71 (MQ=255) aaCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttg < 1:1356159/70‑1 (MQ=255) aCCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttgg < 1:225850/70‑1 (MQ=255) cATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGaaa > 1:2938307/1‑51 (MQ=255) cATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggttg < 1:1703518/71‑1 (MQ=255) cATTTTGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggttg < 1:2509976/71‑1 (MQ=255) tttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttggt > 1:1881712/1‑66 (MQ=255) ttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGgcg > 1:869941/1‑41 (MQ=255) ttGATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTtgctg > 1:34166/1‑70 (MQ=255) tGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTtgctg > 1:2919281/1‑69 (MQ=255) gATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCa < 1:2875392/70‑1 (MQ=255) gATATTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCa < 1:2687120/70‑1 (MQ=255) atatTCCTGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt < 1:2867589/71‑1 (MQ=255) tatTCCTGGTTTCCATCTCACCGGAACTCGGCAgg > 1:3636027/1‑35 (MQ=255) ttCCTGGTTACCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAttt < 1:1438856/70‑1 (MQ=255) ttCATGGTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAttt > 1:1158747/1‑70 (MQ=255) ccTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATtgatg > 1:1393544/1‑49 (MQ=255) cTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGaa < 1:3087138/70‑1 (MQ=255) ggTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAAt > 1:1331072/1‑71 (MQ=255) gTTTCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATgttgg > 1:3616178/1‑52 (MQ=255) tCCATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:1603563/1‑71 (MQ=255) tCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:1514878/1‑71 (MQ=255) tCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:581913/1‑71 (MQ=255) tCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:281099/1‑71 (MQ=255) tCCATACCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:824338/1‑71 (MQ=255) tCCATACCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:828791/1‑71 (MQ=255) ccATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg > 1:3339411/1‑70 (MQ=255) ccATCCCACAGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAg < 1:3559172/70‑1 (MQ=255) cATCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGt < 1:639935/70‑1 (MQ=255) aTCTCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAAt < 1:3751938/56‑1 (MQ=255) ccACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGa > 1:2126883/1‑70 (MQ=255) | CTGATGAAAGAAGATATTTTATTACTGATTGAACCTATTAACCATTTTGATATTCCTGGTTTCCATCCCACCGGAACTCGGCAGGCGCTGAAATTGATTGATGATGTTGGTTGCTGCAATTTGAAAATTCAGTATGA > minE/349893‑350029 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |